5ddv
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 5ddv is ON HOLD Authors: Jin, Y., Liu, Z.C., Wang, G.G. Description: Crystal structure of IspD from Bacillus subtilis at 2.30 Angstroms resolution,...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of IspD from Bacillus subtilis at 2.30 Angstroms resolution, crystal form II== | |
| + | <StructureSection load='5ddv' size='340' side='right'caption='[[5ddv]], [[Resolution|resolution]] 2.30Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5ddv]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis_subsp._subtilis_str._168 Bacillus subtilis subsp. subtilis str. 168]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DDV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5DDV FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3Å</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ddv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ddv OCA], [https://pdbe.org/5ddv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ddv RCSB], [https://www.ebi.ac.uk/pdbsum/5ddv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ddv ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/ISPD_BACSU ISPD_BACSU] Catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP). | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | 2-C-Methyl-D-erythritol-4-phosphate cytidyltransferase (IspD) is an essential enzyme in the mevalonate-independent pathway of isoprenoid biosynthesis. This enzyme catalyzes 2-C-Methyl-d-erythritol 4-phosphate (MEP) and cytosine triphosphate (CTP) to 4-diphosphocytidyl-2-C-methyl-d-erythritol (CDPME) and inorganic pyrophosphate (PPi). Bacillus subtilis was a kind of excellent isoprene producer. However, the studies on the key enzymes of MEP pathway in B. subtilis were still absent. In this work, the crystal structures of IspD and IspD complexed with CTP from B.subtilis were determined. For the first time, the intact P-loop was observed in the apo structure of IspD enzyme. Structural comparisons revealed that the concerted movements of the P-loop and loops close to the active site were essential in the reaction catalyzed by IspD. Meanwhile, kinetic analysis showed that the CTP hydrolytic activity of IspD from B.subtilis was over two times higher than that from Escherichia coli. These results will be useful for future target-based screening of potential inhibitors and the metabolic engineering for isoprenoid biosynthesis. | ||
| - | + | A structural and functional study on the 2-C-methyl-d-erythritol-4-phosphate cytidyltransferase (IspD) from Bacillus subtilis.,Jin Y, Liu Z, Li Y, Liu W, Tao Y, Wang G Sci Rep. 2016 Nov 8;6:36379. doi: 10.1038/srep36379. PMID:27821871<ref>PMID:27821871</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 5ddv" style="background-color:#fffaf0;"></div> |
| - | [[Category: Liu | + | |
| - | [[Category: | + | ==See Also== |
| + | *[[MEP cytidylyltransferase 3D structures|MEP cytidylyltransferase 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Bacillus subtilis subsp. subtilis str. 168]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Jin Y]] | ||
| + | [[Category: Liu ZC]] | ||
| + | [[Category: Wang GG]] | ||
Current revision
Crystal structure of IspD from Bacillus subtilis at 2.30 Angstroms resolution, crystal form II
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