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1hi5

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[[Image:1hi5.gif|left|200px]]
 
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{{Structure
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==Eosinophil-derived Neurotoxin (EDN) - Adenosine-5'-Diphosphate Complex==
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|PDB= 1hi5 |SIZE=350|CAPTION= <scene name='initialview01'>1hi5</scene>, resolution 1.80&Aring;
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<StructureSection load='1hi5' size='340' side='right'caption='[[1hi5]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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|SITE= <scene name='pdbsite=ADP:Adp+Binding+Site+For+Chain+A'>ADP</scene>
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=ADP:ADENOSINE-5&#39;-DIPHOSPHATE'>ADP</scene>
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<table><tr><td colspan='2'>[[1hi5]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HI5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1HI5 FirstGlance]. <br>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Pancreatic_ribonuclease Pancreatic ribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.5 3.1.27.5]
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene></td></tr>
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}}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1hi5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hi5 OCA], [https://pdbe.org/1hi5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1hi5 RCSB], [https://www.ebi.ac.uk/pdbsum/1hi5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1hi5 ProSAT]</span></td></tr>
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</table>
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'''EOSINOPHIL-DERIVED NEUROTOXIN (EDN)-ADENOSINE-5'-DIPHOSPHATE COMPLEX'''
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== Function ==
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[https://www.uniprot.org/uniprot/RNAS2_HUMAN RNAS2_HUMAN] This is a non-secretory ribonuclease. It is a pyrimidine specific nuclease with a slight preference for U. Cytotoxin and helminthotoxin. Selectively chemotactic for dendritic cells. Possesses a wide variety of biological activities.<ref>PMID:3458170</ref> <ref>PMID:12578357</ref>
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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Eosinophil-derived neurotoxin (EDN), a basic ribonuclease found in the large specific granules of eosinophils, belongs to the pancreatic RNase A family. Although its physiological function is still unclear, it has been shown that EDN is a neurotoxin capable of inducing the Gordon phenomenon in rabbits. EDN is also a potent helminthotoxin and can mediate antiviral activity of eosinophils against isolated virions of the respiratory syncytial virus. EDN is a catalytically efficient RNase sharing similar substrate specificity with pancreatic RNase A with its ribonucleolytic activity being absolutely essential for its neurotoxic, helminthotoxic, and antiviral activities. The crystal structure of recombinant human EDN in the unliganded form has been determined previously (Mosimann, S. C., Newton, D. L., Youle, R. J., and James, M. N. G. (1996) J. Mol. Biol. 260, 540-552). We have now determined high resolution (1.8 A) crystal structures for EDN in complex with adenosine-3',5'-diphosphate (3',5'-ADP), adenosine-2',5'-di-phosphate (2',5'-ADP), adenosine-5'-diphosphate (5'-ADP) as well as for a native structure in the presence of sulfate refined at 1.6 A. The inhibition constant of these mononucleotides for EDN has been determined. The structures present the first detailed picture of differences between EDN and RNase A in substrate recognition at the ribonucleolytic active site. They also provide a starting point for the design of tight-binding inhibitors, which may be used to restrain the RNase activity of EDN.
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Check<jmol>
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<jmolCheckbox>
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==Disease==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hi/1hi5_consurf.spt"</scriptWhenChecked>
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Known disease associated with this structure: High density lipoprotein cholesterol level QTL 7 OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=131240 131240]]
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==About this Structure==
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</jmolCheckbox>
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1HI5 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HI5 OCA].
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1hi5 ConSurf].
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<div style="clear:both"></div>
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==Reference==
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== References ==
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Mapping the ribonucleolytic active site of eosinophil-derived neurotoxin (EDN). High resolution crystal structures of EDN complexes with adenylic nucleotide inhibitors., Leonidas DD, Boix E, Prill R, Suzuki M, Turton R, Minson K, Swaminathan GJ, Youle RJ, Acharya KR, J Biol Chem. 2001 May 4;276(18):15009-17. Epub 2001 Jan 11. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11154698 11154698]
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<references/>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Pancreatic ribonuclease]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Acharya KR]]
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[[Category: Acharya, K R.]]
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[[Category: Boix E]]
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[[Category: Boix, E.]]
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[[Category: Leonidas DD]]
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[[Category: Leonidas, D D.]]
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[[Category: Minson K]]
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[[Category: Minson, K.]]
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[[Category: Prill R]]
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[[Category: Prill, R.]]
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[[Category: Suzuki M]]
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[[Category: Suzuki, M.]]
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[[Category: Swaminathan GJ]]
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[[Category: Swaminathan, G J.]]
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[[Category: Turton R]]
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[[Category: Turton, R.]]
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[[Category: Youle RJ]]
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[[Category: Youle, R J.]]
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[[Category: ADP]]
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[[Category: ribonuclease]]
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[[Category: rnase us]]
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[[Category: rnase-2]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 23 12:05:29 2008''
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Current revision

Eosinophil-derived Neurotoxin (EDN) - Adenosine-5'-Diphosphate Complex

PDB ID 1hi5

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