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1c1l

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LACTOSE-LIGANDED CONGERIN I

Structural highlights

1c1l is a 1 chain structure with sequence from Conger myriaster. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.5Å
Ligands:	, , ,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

LEG1_CONMY This protein binds beta-galactoside. Its physiological function is not yet known.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

BACKGROUND: Congerin I is a member of the galectin (animal beta-galactoside-binding lectin) family and is found in the skin mucus of conger eel. The galectin family proteins perform a variety of biological activities. Because of its histological localization and activity against marine bacteria and starfish embryos, congerin I is thought to take part in the eels' biological defense system against parasites. RESULTS: The crystal structure of congerin I has been determined in both lactose-liganded and ligand-free forms to 1. 5 A and 1.6 A resolution, respectively. The protein is a homodimer of 15 kDa subunits. Congerin I has a beta-sheet topology that is markedly different from those of known relatives. One of the beta-strands is exchanged between two identical subunits. This strand swap might increase the dimer stability. Of the known galectin complexes, congerin I forms the most extensive interaction with lactose molecules. Most of these interactions are substituted by similar interactions with water molecules, including a pi-electron hydrogen bond, in the ligand-free form. This observation indicates an increased affinity of congerin I for the ligand. CONCLUSIONS: The genes for congerin I and an isoform, congerin II, are known to have evolved under positive selection pressure. The strand swap and the modification in the carbohydrate-binding site might enhance the cross-linking activity, and should be the most apparent consequence of positive selection. The protein has been adapted to functioning in skin mucus that is in direct contact with surrounding environments by an enhancement in cross-linking activity. The structure of congerin I demonstrates the emergence of a new structure class by accelerated evolution under selection pressure.

High-resolution structure of the conger eel galectin, congerin I, in lactose-liganded and ligand-free forms: emergence of a new structure class by accelerated evolution.,Shirai T, Mitsuyama C, Niwa Y, Matsui Y, Hotta H, Yamane T, Kamiya H, Ishii C, Ogawa T, Muramoto K Structure. 1999 Oct 15;7(10):1223-33. PMID:10545323^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Shirai T, Mitsuyama C, Niwa Y, Matsui Y, Hotta H, Yamane T, Kamiya H, Ishii C, Ogawa T, Muramoto K. High-resolution structure of the conger eel galectin, congerin I, in lactose-liganded and ligand-free forms: emergence of a new structure class by accelerated evolution. Structure. 1999 Oct 15;7(10):1223-33. PMID:10545323

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1c1l"

@@ Line 1: / Line 1: @@
 ==LACTOSE-LIGANDED CONGERIN I==
-<StructureSection load='1c1l' size='340' side='right' caption='[[1c1l]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
+<StructureSection load='1c1l' size='340' side='right'caption='[[1c1l]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1c1l]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Conger_myriaster Conger myriaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1C1L OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1C1L FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1c1l]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Conger_myriaster Conger myriaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1C1L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1C1L FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
-<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=PRD_900004:beta-lactose'>PRD_900004</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1c1l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1c1l OCA], [http://pdbe.org/1c1l PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1c1l RCSB], [http://www.ebi.ac.uk/pdbsum/1c1l PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1c1l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1c1l OCA], [https://pdbe.org/1c1l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1c1l RCSB], [https://www.ebi.ac.uk/pdbsum/1c1l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1c1l ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/LEG1_CONMY LEG1_CONMY]] This protein binds beta-galactoside. Its physiological function is not yet known.
+[https://www.uniprot.org/uniprot/LEG1_CONMY LEG1_CONMY] This protein binds beta-galactoside. Its physiological function is not yet known.
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
 Check<jmol>
   <jmolCheckbox>
-    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/c1/1c1l_consurf.spt"</scriptWhenChecked>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/c1/1c1l_consurf.spt"</scriptWhenChecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
   </jmolCheckbox>
-</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1c1l ConSurf].
 <div style="clear:both"></div>
 <div style="background-color:#fffaf0;">
@@ Line 33: / Line 34: @@
 </StructureSection>
 [[Category: Conger myriaster]]
-[[Category: Hotta, H]]
+[[Category: Large Structures]]
-[[Category: Ishii, C]]
+[[Category: Hotta H]]
-[[Category: Kamiya, H]]
+[[Category: Ishii C]]
-[[Category: Matsui, Y]]
+[[Category: Kamiya H]]
-[[Category: Mitsuyama, C]]
+[[Category: Matsui Y]]
-[[Category: Muramoto, K]]
+[[Category: Mitsuyama C]]
-[[Category: Niwa, Y]]
+[[Category: Muramoto K]]
-[[Category: Ogawa, T]]
+[[Category: Niwa Y]]
-[[Category: Shirai, T]]
+[[Category: Ogawa T]]
-[[Category: Yamane, T]]
+[[Category: Shirai T]]
-[[Category: Beta-galactose-binding]]
+[[Category: Yamane T]]
-[[Category: Galectin]]
-[[Category: Lectin]]
-[[Category: Sugar binding protein]]

1c1l

From Proteopedia

Current revision

LACTOSE-LIGANDED CONGERIN I

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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