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1mdo

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[[Image:1mdo.gif|left|200px]]
 
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{{Structure
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==Crystal structure of ArnB aminotransferase with pyridomine 5' phosphate==
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|PDB= 1mdo |SIZE=350|CAPTION= <scene name='initialview01'>1mdo</scene>, resolution 1.70&Aring;
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<StructureSection load='1mdo' size='340' side='right'caption='[[1mdo]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=PMP:4&#39;-DEOXY-4&#39;-AMINOPYRIDOXAL-5&#39;-PHOSPHATE'>PMP</scene>
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<table><tr><td colspan='2'>[[1mdo]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_Typhimurium Salmonella enterica subsp. enterica serovar Typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MDO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MDO FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=PMP:4-DEOXY-4-AMINOPYRIDOXAL-5-PHOSPHATE'>PMP</scene></td></tr>
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}}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mdo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mdo OCA], [https://pdbe.org/1mdo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mdo RCSB], [https://www.ebi.ac.uk/pdbsum/1mdo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mdo ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/ARNB_SALTY ARNB_SALTY] Catalyzes the conversion of UDP-4-keto-arabinose (UDP-Ara4O) to UDP-4-amino-4-deoxy-L-arabinose (UDP-L-Ara4N). The modified arabinose is attached to lipid A and is required for resistance to polymyxin and cationic antimicrobial peptides (By similarity).[HAMAP-Rule:MF_01167]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/md/1mdo_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mdo ConSurf].
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<div style="clear:both"></div>
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'''Crystal structure of ArnB aminotransferase with pyridomine 5' phosphate'''
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==See Also==
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*[[Aminotransferase 3D structures|Aminotransferase 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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Lipid A modification with 4-amino-4-deoxy-L-arabinose confers on certain pathogenic bacteria, such as Salmonella, resistance to cationic antimicrobial peptides, including those derived from the innate immune system. ArnB catalysis of amino group transfer from glutamic acid to the 4"-position of a UDP-linked ketopyranose molecule to form UDP-4-amino-4-deoxy-L-arabinose represents a key step in the lipid A modification pathway. Structural and functional studies of the ArnB aminotransferase were undertaken by combining X-ray crystallography with biochemical analyses. High-resolution crystal structures were solved for two native forms and one covalently inhibited form of S. typhimurium ArnB. These structures permitted identification of key residues involved in substrate binding and catalysis, including a rarely observed nonprolyl cis peptide bond in the active site.
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[[Category: Large Structures]]
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[[Category: Salmonella enterica subsp. enterica serovar Typhimurium]]
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==About this Structure==
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[[Category: Badger J]]
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1MDO is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Salmonella_typhimurium Salmonella typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MDO OCA].
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[[Category: Buchanan MD]]
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[[Category: Buchanan SG]]
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==Reference==
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[[Category: Christopher JA]]
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Structural studies of Salmonella typhimurium ArnB (PmrH) aminotransferase: a 4-amino-4-deoxy-L-arabinose lipopolysaccharide-modifying enzyme., Noland BW, Newman JM, Hendle J, Badger J, Christopher JA, Tresser J, Buchanan MD, Wright TA, Rutter ME, Sanderson WE, Muller-Dieckmann HJ, Gajiwala KS, Buchanan SG, Structure. 2002 Nov;10(11):1569-80. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12429098 12429098]
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[[Category: Gajiwala K]]
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[[Category: Salmonella typhimurium]]
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[[Category: Hendle J]]
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[[Category: Single protein]]
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[[Category: Muller-Dieckmann H-J]]
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[[Category: Badger, J.]]
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[[Category: Newman JM]]
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[[Category: Buchanan, M D.]]
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[[Category: Noland BW]]
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[[Category: Buchanan, S G.]]
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[[Category: Rutter ME]]
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[[Category: Christopher, J A.]]
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[[Category: Sanderson WE]]
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[[Category: Gajiwala, K.]]
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[[Category: Sauder JM]]
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[[Category: Hendle, J.]]
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[[Category: Tresser J]]
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[[Category: Muller-Dieckmann, H J.]]
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[[Category: Wright T]]
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[[Category: Newman, J M.]]
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[[Category: Noland, B W.]]
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[[Category: Rutter, M E.]]
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[[Category: Sanderson, W E.]]
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[[Category: Tresser, J.]]
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[[Category: Wright, T.]]
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[[Category: PMP]]
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[[Category: type 1 aminotransferase fold]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 23 12:45:00 2008''
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Current revision

Crystal structure of ArnB aminotransferase with pyridomine 5' phosphate

PDB ID 1mdo

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