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1miy

From Proteopedia

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[[Image:1miy.gif|left|200px]]
 
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{{Structure
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==Crystal structure of Bacillus stearothermophilus CCA-adding enzyme in complex with CTP==
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|PDB= 1miy |SIZE=350|CAPTION= <scene name='initialview01'>1miy</scene>, resolution 3.52&Aring;
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<StructureSection load='1miy' size='340' side='right'caption='[[1miy]], [[Resolution|resolution]] 3.52&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> and <scene name='pdbligand=CTP:CYTIDINE-5&#39;-TRIPHOSPHATE'>CTP</scene>
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<table><tr><td colspan='2'>[[1miy]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MIY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MIY FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.52&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CTP:CYTIDINE-5-TRIPHOSPHATE'>CTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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}}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1miy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1miy OCA], [https://pdbe.org/1miy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1miy RCSB], [https://www.ebi.ac.uk/pdbsum/1miy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1miy ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CCA_GEOSE CCA_GEOSE]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mi/1miy_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1miy ConSurf].
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<div style="clear:both"></div>
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'''Crystal structure of Bacillus stearothermophilus CCA-adding enzyme in complex with CTP'''
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==See Also==
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*[[CCA-adding enzyme 3D structures|CCA-adding enzyme 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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CCA-adding enzymes polymerize CCA onto the 3' terminus of immature tRNAs without using a nucleic acid template. The 3.0 A resolution crystal structures of the CCA-adding enzyme from Bacillus stearothermophilus and its complexes with ATP or CTP reveal a seahorse-shaped subunit consisting of four domains: head, neck, body, and tail. The head is structurally homologous to the palm domain of DNA polymerase beta but has additional structural features and functions. The neck, body, and tail represent new protein folding motifs. The neck provides a specific template for the incoming ATP or CTP, whereas the body and tail may bind tRNA. Each subunit has one active site capable of switching its base specificity between ATP and CTP, an important component of the CCA-adding mechanism.
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==About this Structure==
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1MIY is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MIY OCA].
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==Reference==
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Crystal structures of the Bacillus stearothermophilus CCA-adding enzyme and its complexes with ATP or CTP., Li F, Xiong Y, Wang J, Cho HD, Tomita K, Weiner AM, Steitz TA, Cell. 2002 Dec 13;111(6):815-24. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12526808 12526808]
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[[Category: Geobacillus stearothermophilus]]
[[Category: Geobacillus stearothermophilus]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Cho, H D.]]
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[[Category: Cho HD]]
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[[Category: Li, F.]]
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[[Category: Li F]]
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[[Category: Steitz, T A.]]
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[[Category: Steitz TA]]
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[[Category: Wang, J.]]
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[[Category: Wang J]]
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[[Category: Weiner, A M.]]
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[[Category: Weiner AM]]
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[[Category: Xiong, Y.]]
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[[Category: Xiong Y]]
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[[Category: CTP]]
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[[Category: MG]]
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[[Category: cca-adding enzyme]]
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[[Category: trna nucleotidyltransferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 23 12:46:03 2008''
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Current revision

Crystal structure of Bacillus stearothermophilus CCA-adding enzyme in complex with CTP

PDB ID 1miy

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