|
|
| (3 intermediate revisions not shown.) |
| Line 1: |
Line 1: |
| | + | |
| | ==Backbone structure of human membrane protein TMEM14A from NOE data== | | ==Backbone structure of human membrane protein TMEM14A from NOE data== |
| - | <StructureSection load='2loo' size='340' side='right' caption='[[2loo]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='2loo' size='340' side='right'caption='[[2loo]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2loo]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LOO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2LOO FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2loo]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LOO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2LOO FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2lom|2lom]], [[2lon|2lon]], [[2lop|2lop]], [[2loq|2loq]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">TMEM14A, C6orf73, PTD011 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2loo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2loo OCA], [https://pdbe.org/2loo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2loo RCSB], [https://www.ebi.ac.uk/pdbsum/2loo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2loo ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2loo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2loo OCA], [http://pdbe.org/2loo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2loo RCSB], [http://www.ebi.ac.uk/pdbsum/2loo PDBsum]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/TM14A_HUMAN TM14A_HUMAN] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
| Line 20: |
Line 22: |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| - | [[Category: Choe, S]] | + | [[Category: Large Structures]] |
| - | [[Category: Eichmann, C]] | + | [[Category: Choe S]] |
| - | [[Category: Klammt, C]] | + | [[Category: Eichmann C]] |
| - | [[Category: Maslennikov, I]] | + | [[Category: Klammt C]] |
| - | [[Category: Riek, R]] | + | [[Category: Maslennikov I]] |
| - | [[Category: Helical bundle]]
| + | [[Category: Riek R]] |
| - | [[Category: Membrane protein]]
| + | |
| Structural highlights
Function
TM14A_HUMAN
Publication Abstract from PubMed
Although nearly half of today's major pharmaceutical drugs target human integral membrane proteins (hIMPs), only 30 hIMP structures are currently available in the Protein Data Bank, largely owing to inefficiencies in protein production. Here we describe a strategy for the rapid structure determination of hIMPs, using solution NMR spectroscopy with systematically labeled proteins produced via cell-free expression. We report new backbone structures of six hIMPs, solved in only 18 months from 15 initial targets. Application of our protocols to an additional 135 hIMPs with molecular weight <30 kDa yielded 38 hIMPs suitable for structural characterization by solution NMR spectroscopy without additional optimization.
Facile backbone structure determination of human membrane proteins by NMR spectroscopy.,Klammt C, Maslennikov I, Bayrhuber M, Eichmann C, Vajpai N, Chiu EJ, Blain KY, Esquivies L, Kwon JH, Balana B, Pieper U, Sali A, Slesinger PA, Kwiatkowski W, Riek R, Choe S Nat Methods. 2012 May 20;9(8):834-9. doi: 10.1038/nmeth.2033. PMID:22609626[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Klammt C, Maslennikov I, Bayrhuber M, Eichmann C, Vajpai N, Chiu EJ, Blain KY, Esquivies L, Kwon JH, Balana B, Pieper U, Sali A, Slesinger PA, Kwiatkowski W, Riek R, Choe S. Facile backbone structure determination of human membrane proteins by NMR spectroscopy. Nat Methods. 2012 May 20;9(8):834-9. doi: 10.1038/nmeth.2033. PMID:22609626 doi:10.1038/nmeth.2033
|
