|
|
| (4 intermediate revisions not shown.) |
| Line 1: |
Line 1: |
| | + | |
| | ==MICROCIN LEADER PEPTIDE FROM E. COLI, NMR, 25 STRUCTURES== | | ==MICROCIN LEADER PEPTIDE FROM E. COLI, NMR, 25 STRUCTURES== |
| - | <StructureSection load='2mlp' size='340' side='right' caption='[[2mlp]], [[NMR_Ensembles_of_Models | 25 NMR models]]' scene=''> | + | <StructureSection load='2mlp' size='340' side='right'caption='[[2mlp]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2mlp]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MLP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2MLP FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2mlp]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MLP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MLP FirstGlance]. <br> |
| - | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 25 models</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2mlp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mlp OCA], [http://pdbe.org/2mlp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2mlp RCSB], [http://www.ebi.ac.uk/pdbsum/2mlp PDBsum]</span></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2mlp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mlp OCA], [https://pdbe.org/2mlp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2mlp RCSB], [https://www.ebi.ac.uk/pdbsum/2mlp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2mlp ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/MCBA_ECOLI MCBA_ECOLI]] Affects biofilm formation and mucoidy.<ref>PMID:18309357</ref> | + | [https://www.uniprot.org/uniprot/MCBA_ECOLX MCBA_ECOLX] This glycine-rich peptide antibiotic inhibits DNA replication in many enteric bacteria, that leads to induction of the SOS repair system, massive DNA degradation and cell death. B17 inhibits type II topoisomerase by trapping an enzyme - DNA cleavable complex.<ref>PMID:1846808</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
| Line 21: |
Line 23: |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Bacillus coli migula 1895]] | + | [[Category: Escherichia coli]] |
| - | [[Category: Baleja, J D]] | + | [[Category: Large Structures]] |
| - | [[Category: Kim, S]] | + | [[Category: Baleja JD]] |
| - | [[Category: Roy, R Sinha]] | + | [[Category: Kim S]] |
| - | [[Category: Walsh, C T]] | + | [[Category: Sinha Roy R]] |
| - | [[Category: Antibiotic]] | + | [[Category: Walsh CT]] |
| - | [[Category: Leader peptide]]
| + | |
| - | [[Category: Propeptide]]
| + | |
| Structural highlights
Function
MCBA_ECOLX This glycine-rich peptide antibiotic inhibits DNA replication in many enteric bacteria, that leads to induction of the SOS repair system, massive DNA degradation and cell death. B17 inhibits type II topoisomerase by trapping an enzyme - DNA cleavable complex.[1]
Publication Abstract from PubMed
BACKGROUND: The peptide antibiotic microcin B17 (MccB17) contains oxazole and thiazole heterocycles formed by the post-translational modification of four cysteine and four serine residues. An amino-terminal propeptide targets the 69 amino acid precursor of MccB17 (preproMccB17) to the heterocyclization enzyme MccB17 synthetase. The mode of synthetase recognition has been unclear, because there has been limited structural information available on the MccB17 propeptide to date. RESULTS: The solution structure of the MccB17 propeptide (McbA1-26), determined using nuclear magnetic resonance, reveals that McbA1-26 is an amphipathic alpha helix. Mutational analysis of 13 propeptide residues showed that Phe8 and Leu12 are essential residues for MccB17 synthetase recognition. A domain of the propeptide was putatively identified as the region that interacts with the synthetase. CONCLUSIONS: MccB17 synthetase recognizes key hydrophobic residues within a helical propeptide, allowing the selective heterocyclization of downstream cysteine and serine residues in preproMccB17. The determination of the solution structure of the propeptide should facilitate the investigation of other functions of the propeptide, including a potential role in antibiotic secretion.
Role of the microcin B17 propeptide in substrate recognition: solution structure and mutational analysis of McbA1-26.,Roy RS, Kim S, Baleja JD, Walsh CT Chem Biol. 1998 Apr;5(4):217-28. PMID:9545435[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Vizan JL, Hernandez-Chico C, del Castillo I, Moreno F. The peptide antibiotic microcin B17 induces double-strand cleavage of DNA mediated by E. coli DNA gyrase. EMBO J. 1991 Feb;10(2):467-76. PMID:1846808
- ↑ Roy RS, Kim S, Baleja JD, Walsh CT. Role of the microcin B17 propeptide in substrate recognition: solution structure and mutational analysis of McbA1-26. Chem Biol. 1998 Apr;5(4):217-28. PMID:9545435
|
