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| | ==Trn- peptide of the two-component bacteriocin Thuricin CD== | | ==Trn- peptide of the two-component bacteriocin Thuricin CD== |
| - | <StructureSection load='2l9x' size='340' side='right' caption='[[2l9x]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='2l9x' size='340' side='right'caption='[[2l9x]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2l9x]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_cereus_95/8201 Bacillus cereus 95/8201]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L9X OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2L9X FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2l9x]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_cereus_95/8201 Bacillus cereus 95/8201]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L9X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2L9X FirstGlance]. <br> |
| - | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=DTH:D-THREONINE'>DTH</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 20 models</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2la0|2la0]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DTH:D-THREONINE'>DTH</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2l9x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l9x OCA], [http://pdbe.org/2l9x PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2l9x RCSB], [http://www.ebi.ac.uk/pdbsum/2l9x PDBsum]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2l9x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l9x OCA], [https://pdbe.org/2l9x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2l9x RCSB], [https://www.ebi.ac.uk/pdbsum/2l9x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2l9x ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Bacillus cereus 95/8201]] | | [[Category: Bacillus cereus 95/8201]] |
| - | [[Category: Hill, C]] | + | [[Category: Large Structures]] |
| - | [[Category: Mckay, R T]] | + | [[Category: Hill C]] |
| - | [[Category: Ross, R P]]
| + | [[Category: Mckay RT]] |
| - | [[Category: Sit, C S]]
| + | [[Category: Ross RP]] |
| - | [[Category: Vederas, J C]] | + | [[Category: Sit CS]] |
| - | [[Category: Antimicrobial protein]] | + | [[Category: Vederas JC]] |
| - | [[Category: Crosslinked]] | + | |
| - | [[Category: Helical loop]] | + | |
| - | [[Category: Post-translationally modified]]
| + | |
| - | [[Category: Thioether bridge]]
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| Structural highlights
Publication Abstract from PubMed
Thuricin CD is an antimicrobial factor that consists of two peptides, Trn-alpha and Trn-beta, that exhibit synergistic activity against drug resistant strains of Clostridium difficile. Trn-alpha and Trn-beta each possess three sulfur to alpha-carbon thioether bridges for which the stereochemistry is unknown. This report presents the three-dimensional solution structures of Trn-alpha and Trn-beta. Structure calculations were performed for the eight possible stereoisomers of each peptide based on the same NMR data. The structure of the stereoisomer that best fit the experimental data was chosen as the representative structure for each peptide. It was determined that Trn-alpha has L-stereochemistry at Ser21 (alpha-R), L-stereochemistry at Thr25 (alpha-R), and D-stereochemistry at Thr28 (alpha-S) (an LLD isomer). Trn-beta was also found to be the LLD isomer, with L-stereochemistry at Thr21 (alpha-R), L-stereochemistry at Ala25 (alpha-R), and D-stereochemistry at Tyr28 (alpha-S).
The 3D structure of thuricin CD, a two-component bacteriocin with cysteine sulfur to alpha-carbon cross-links.,Sit CS, McKay RT, Hill C, Ross RP, Vederas JC J Am Chem Soc. 2011 May 25;133(20):7680-3. Epub 2011 Apr 28. PMID:21526839[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Sit CS, McKay RT, Hill C, Ross RP, Vederas JC. The 3D structure of thuricin CD, a two-component bacteriocin with cysteine sulfur to alpha-carbon cross-links. J Am Chem Soc. 2011 May 25;133(20):7680-3. Epub 2011 Apr 28. PMID:21526839 doi:10.1021/ja201802f
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