1n6m

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[[Image:1n6m.gif|left|200px]]
 
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{{Structure
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==Rotation of the stalk/neck and one head in a new crystal structure of the kinesin motor protein, Ncd==
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|PDB= 1n6m |SIZE=350|CAPTION= <scene name='initialview01'>1n6m</scene>, resolution 2.5&Aring;
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<StructureSection load='1n6m' size='340' side='right'caption='[[1n6m]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> and <scene name='pdbligand=ADP:ADENOSINE-5&#39;-DIPHOSPHATE'>ADP</scene>
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<table><tr><td colspan='2'>[[1n6m]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N6M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1N6M FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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}}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1n6m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n6m OCA], [https://pdbe.org/1n6m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1n6m RCSB], [https://www.ebi.ac.uk/pdbsum/1n6m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1n6m ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/NCD_DROME NCD_DROME] NCD is required for normal chromosomal segregation in meiosis, in females, and in early mitotic divisions of the embryo. The NCD motor activity is directed toward the microtubule's minus end.<ref>PMID:2146510</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/n6/1n6m_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1n6m ConSurf].
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<div style="clear:both"></div>
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'''Rotation of the stalk/neck and one head in a new crystal structure of the kinesin motor protein, Ncd'''
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==See Also==
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*[[Kinesin 3D Structures|Kinesin 3D Structures]]
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== References ==
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==Overview==
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<references/>
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Molecular motors undergo conformational changes to produce force and move along cytoskeletal filaments. Structural changes have been detected in kinesin motors; however, further changes are expected because previous crystal structures are in the same or closely related conformations. We report here a 2.5 A crystal structure of the minus-end kinesin, Ncd, with the coiled-coil stalk/neck and one head rotated by approximately 75 degrees relative to the other head. The two heads are asymmetrically positioned with respect to the stalk and show asymmetry of nucleotide state: one head is fully occupied, but the other is unstably bound to ADP. Unlike previous structures, our new atomic model can be fit into cryoelectron microscopy density maps of the motor attached to microtubules, where it appears to resemble a one-head-bound motor with the stalk rotated towards the minus end. Interactions between neck and motor core residues, observed in the head that moves with the stalk, are disrupted in the other head, permitting rotation of the stalk/neck. The rotation could represent a force-producing stroke that directs the motor to the minus end.
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__TOC__
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</StructureSection>
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==About this Structure==
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1N6M is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N6M OCA].
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==Reference==
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Rotation of the stalk/neck and one head in a new crystal structure of the kinesin motor protein, Ncd., Yun M, Bronner CE, Park CG, Cha SS, Park HW, Endow SA, EMBO J. 2003 Oct 15;22(20):5382-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14532111 14532111]
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[[Category: Drosophila melanogaster]]
[[Category: Drosophila melanogaster]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Bronner, C E.]]
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[[Category: Bronner CE]]
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[[Category: Cha, S S.]]
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[[Category: Cha S-S]]
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[[Category: Endow, S A.]]
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[[Category: Endow SA]]
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[[Category: Park, C G.]]
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[[Category: Park C-G]]
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[[Category: Park, H W.]]
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[[Category: Park H-W]]
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[[Category: Yun, M.]]
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[[Category: Yun M]]
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[[Category: ADP]]
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[[Category: MG]]
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[[Category: kinesin]]
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[[Category: microtubule]]
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[[Category: motor]]
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[[Category: ncd]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 23 12:51:46 2008''
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Current revision

Rotation of the stalk/neck and one head in a new crystal structure of the kinesin motor protein, Ncd

PDB ID 1n6m

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