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| | ==SOLUTION CONFORMATION OF A-CONOTOXIN AUIB== | | ==SOLUTION CONFORMATION OF A-CONOTOXIN AUIB== |
| - | <StructureSection load='1dg2' size='340' side='right' caption='[[1dg2]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='1dg2' size='340' side='right'caption='[[1dg2]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1dg2]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Conus_aulicus Conus aulicus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DG2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1DG2 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1dg2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Conus_aulicus Conus aulicus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DG2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1DG2 FirstGlance]. <br> |
| - | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 20 models</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1dg2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dg2 OCA], [http://pdbe.org/1dg2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1dg2 RCSB], [http://www.ebi.ac.uk/pdbsum/1dg2 PDBsum]</span></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1dg2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dg2 OCA], [https://pdbe.org/1dg2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1dg2 RCSB], [https://www.ebi.ac.uk/pdbsum/1dg2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1dg2 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/CA1B_CONAL CA1B_CONAL]] Alpha-conotoxins act on postsynaptic membranes, they bind to the nicotinic acetylcholine receptors (nAChR) and thus inhibit them. This toxin blocks mammalian nAChR alpha-3/beta-4 subunits.<ref>PMID:9786965</ref> | + | [https://www.uniprot.org/uniprot/CA1B_CONAL CA1B_CONAL] Alpha-conotoxins act on postsynaptic membranes, they bind to the nicotinic acetylcholine receptors (nAChR) and thus inhibit them. This toxin blocks mammalian nAChR alpha-3/beta-4 subunits.<ref>PMID:9786965</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Conus aulicus]] | | [[Category: Conus aulicus]] |
| - | [[Category: Cho, J H]] | + | [[Category: Large Structures]] |
| - | [[Category: Han, K H]] | + | [[Category: Cho J-H]] |
| - | [[Category: McIntosh, J M]] | + | [[Category: Han K-H]] |
| - | [[Category: Mok, K H]] | + | [[Category: McIntosh JM]] |
| - | [[Category: Olivera, B M]] | + | [[Category: Mok KH]] |
| - | [[Category: Park, K H]] | + | [[Category: Olivera BM]] |
| - | [[Category: A-helix]]
| + | [[Category: Park K-H]] |
| - | [[Category: Toxin]]
| + | |
| - | [[Category: Two disulfide bonds and c-term amidation]]
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| Structural highlights
Function
CA1B_CONAL Alpha-conotoxins act on postsynaptic membranes, they bind to the nicotinic acetylcholine receptors (nAChR) and thus inhibit them. This toxin blocks mammalian nAChR alpha-3/beta-4 subunits.[1]
Publication Abstract from PubMed
The neuronal nicotinic acetylcholine receptors constitute a highly diverse group, with subtypes consisting of pentameric combinations of alpha and beta subunits. alpha-Conotoxins are a homologous series of small peptides that antagonize these receptors. We present the three-dimensional solution structure of alpha-conotoxin AuIB, the first 15-residue alpha-conotoxin known to selectively block the alpha(3)beta(4) nicotinic acetylcholine receptor subtype. The pairwise backbone and heavy-atom root mean square deviation for an ensemble of 20 structures are 0.269 and 0.720 A, respectively. The overall fold of alpha-conotoxin AuIB closely resembles that of the alpha4/7 subfamily alpha-conotoxins. However, the absence of Tyr(15), normally present in other alpha4/7 members, results in tight bending of the backbone at the C terminus and effectively renders Asp(14) to assume the spatial location of Tyr(15) present in other neuronal alpha4/7 alpha-conotoxins. Structural comparison of alpha-conotoxin AuIB with the alpha(3)beta(2) subtype-specific alpha-conotoxin MII shows different electrostatic surface charge distributions, which may be important in differential receptor subtype recognition.
Nuclear magnetic resonance solution conformation of alpha-conotoxin AuIB, an alpha(3)beta(4) subtype-selective neuronal nicotinic acetylcholine receptor antagonist.,Cho JH, Mok KH, Olivera BM, McIntosh JM, Park KH, Han KH J Biol Chem. 2000 Mar 24;275(12):8680-5. PMID:10722709[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Luo S, Kulak JM, Cartier GE, Jacobsen RB, Yoshikami D, Olivera BM, McIntosh JM. alpha-conotoxin AuIB selectively blocks alpha3 beta4 nicotinic acetylcholine receptors and nicotine-evoked norepinephrine release. J Neurosci. 1998 Nov 1;18(21):8571-9. PMID:9786965
- ↑ Cho JH, Mok KH, Olivera BM, McIntosh JM, Park KH, Han KH. Nuclear magnetic resonance solution conformation of alpha-conotoxin AuIB, an alpha(3)beta(4) subtype-selective neuronal nicotinic acetylcholine receptor antagonist. J Biol Chem. 2000 Mar 24;275(12):8680-5. PMID:10722709
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