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1pag

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[[Image:1pag.jpg|left|200px]]
 
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{{Structure
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==THE 2.5 ANGSTROMS STRUCTURE OF POKEWEED ANTIVIRAL PROTEIN==
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|PDB= 1pag |SIZE=350|CAPTION= <scene name='initialview01'>1pag</scene>, resolution 2.8&Aring;
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<StructureSection load='1pag' size='340' side='right'caption='[[1pag]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=FMP:FORMYCIN-5&#39;-MONOPHOSPHATE'>FMP</scene>
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<table><tr><td colspan='2'>[[1pag]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Phytolacca_americana Phytolacca americana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PAG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1PAG FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMP:FORMYCIN-5-MONOPHOSPHATE'>FMP</scene></td></tr>
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}}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1pag FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pag OCA], [https://pdbe.org/1pag PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1pag RCSB], [https://www.ebi.ac.uk/pdbsum/1pag PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1pag ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RIP1_PHYAM RIP1_PHYAM] Inhibits viral infection of plants, and protein synthesis in vitro.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pa/1pag_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1pag ConSurf].
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<div style="clear:both"></div>
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'''THE 2.5 ANGSTROMS STRUCTURE OF POKEWEED ANTIVIRAL PROTEIN'''
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==See Also==
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*[[Ribosome inactivating protein 3D structures|Ribosome inactivating protein 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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The pokeweed antiviral protein (PAP), isolated from the leaves of Phytolacca americana, is one of a family of plant and bacterial ribosome-inhibiting proteins (RIPs) which act as specific N-glycosidases on rRNA. Here we report the three-dimensional structure of PAP determined to 2.5 A resolution by X-ray crystallography. After 14 rounds of refinement, the R factor is 0.17 for 5.0 to 2.5 A data. The protein is homologous with the A chain of ricin and exhibits a very similar folding pattern. The positions of key active site residues are also similar. We also report the 2.8 A structure of PAP complexed with a substrate analog, formycin 5'-monophosphate. As seen previously in ricin, the formycin ring is stacked between invariant tyrosines 72 and 123. Arg179 bonds to N-3 which is thought to be important in catalysis.
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[[Category: Large Structures]]
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==About this Structure==
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1PAG is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Phytolacca_americana Phytolacca americana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PAG OCA].
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==Reference==
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The 2.5 A structure of pokeweed antiviral protein., Monzingo AF, Collins EJ, Ernst SR, Irvin JD, Robertus JD, J Mol Biol. 1993 Oct 20;233(4):705-15. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8411176 8411176]
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[[Category: Phytolacca americana]]
[[Category: Phytolacca americana]]
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[[Category: Single protein]]
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[[Category: Collins EJ]]
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[[Category: Collins, E J.]]
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[[Category: Ernst SR]]
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[[Category: Ernst, S R.]]
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[[Category: Irvin JD]]
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[[Category: Irvin, J D.]]
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[[Category: Monzingo AF]]
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[[Category: Monzingo, A F.]]
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[[Category: Robertus JD]]
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[[Category: Robertus, J D.]]
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[[Category: FMP]]
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[[Category: protein synthesis inhibitor]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 23 13:11:11 2008''
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THE 2.5 ANGSTROMS STRUCTURE OF POKEWEED ANTIVIRAL PROTEIN

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