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| | ==THREE-DIMENSIONAL STRUCTURE OF THE PENTAMERIC HELICAL BUNDLE OF THE ACETYLCHOLINE RECEPTOR M2 TRANSMEMBRANE SEGMENT== | | ==THREE-DIMENSIONAL STRUCTURE OF THE PENTAMERIC HELICAL BUNDLE OF THE ACETYLCHOLINE RECEPTOR M2 TRANSMEMBRANE SEGMENT== |
| - | <StructureSection load='1eq8' size='340' side='right' caption='[[1eq8]], [[NMR_Ensembles_of_Models | 1 NMR models]]' scene=''> | + | <StructureSection load='1eq8' size='340' side='right'caption='[[1eq8]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1eq8]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Pacific_electric_ray Pacific electric ray]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EQ8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1EQ8 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1eq8]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Tetronarce_californica Tetronarce californica]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EQ8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EQ8 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=OH:HYDROXIDE+ION'>OH</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solid-state NMR, 1 model</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1a11|1a11]], [[1cek|1cek]]</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1eq8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eq8 OCA], [https://pdbe.org/1eq8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1eq8 RCSB], [https://www.ebi.ac.uk/pdbsum/1eq8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1eq8 ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1eq8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eq8 OCA], [http://pdbe.org/1eq8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1eq8 RCSB], [http://www.ebi.ac.uk/pdbsum/1eq8 PDBsum]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/ACHD_TORCA ACHD_TORCA]] After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. | + | [https://www.uniprot.org/uniprot/ACHD_TETCF ACHD_TETCF] After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Pacific electric ray]] | + | [[Category: Large Structures]] |
| - | [[Category: Gesell, J J]] | + | [[Category: Tetronarce californica]] |
| - | [[Category: Kim, Y]] | + | [[Category: Gesell JJ]] |
| - | [[Category: Marassi, F M]] | + | [[Category: Kim Y]] |
| - | [[Category: Montal, M]] | + | [[Category: Marassi FM]] |
| - | [[Category: Oblatt-Montal, M]] | + | [[Category: Montal M]] |
| - | [[Category: Opella, S J]] | + | [[Category: Oblatt-Montal M]] |
| - | [[Category: Valente, A P]] | + | [[Category: Opella SJ]] |
| - | [[Category: Helical bundle]]
| + | [[Category: Valente AP]] |
| - | [[Category: Ion-channel]]
| + | |
| - | [[Category: Lipid bilayer]]
| + | |
| - | [[Category: M2]]
| + | |
| - | [[Category: Neurotransmitter receptor]]
| + | |
| - | [[Category: Pentameric bundle]]
| + | |
| - | [[Category: Signaling protein]]
| + | |
| Structural highlights
Function
ACHD_TETCF After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.
Publication Abstract from PubMed
The structures of functional peptides corresponding to the predicted channel-lining M2 segments of the nicotinic acetylcholine receptor (AChR) and of a glutamate receptor of the NMDA subtype (NMDAR) were determined using solution NMR experiments on micelle samples, and solid-state NMR experiments on bilayer samples. Both M2 segments form straight transmembrane alpha-helices with no kinks. The AChR M2 peptide inserts in the lipid bilayer at an angle of 12 degrees relative to the bilayer normal, with a rotation about the helix long axis such that the polar residues face the N-terminal side of the membrane, which is assigned to be intracellular. A model built from these solid-state NMR data, and assuming a symmetric pentameric arrangement of M2 helices, results in a funnel-like architecture for the channel, with the wide opening on the N-terminal intracellular side.
Structures of the M2 channel-lining segments from nicotinic acetylcholine and NMDA receptors by NMR spectroscopy.,Opella SJ, Marassi FM, Gesell JJ, Valente AP, Kim Y, Oblatt-Montal M, Montal M Nat Struct Biol. 1999 Apr;6(4):374-9. PMID:10201407[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Opella SJ, Marassi FM, Gesell JJ, Valente AP, Kim Y, Oblatt-Montal M, Montal M. Structures of the M2 channel-lining segments from nicotinic acetylcholine and NMDA receptors by NMR spectroscopy. Nat Struct Biol. 1999 Apr;6(4):374-9. PMID:10201407 doi:10.1038/7610
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