1s1y

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[[Image:1s1y.jpg|left|200px]]
 
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{{Structure
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==Photoactivated chromophore conformation in Photoactive Yellow Protein (E46Q mutant) from 10 microseconds to 3 milliseconds==
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|PDB= 1s1y |SIZE=350|CAPTION= <scene name='initialview01'>1s1y</scene>, resolution 1.6&Aring;
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<StructureSection load='1s1y' size='340' side='right'caption='[[1s1y]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=HC4:4&#39;-HYDROXYCINNAMIC ACID'>HC4</scene>
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<table><tr><td colspan='2'>[[1s1y]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Halorhodospira_halophila Halorhodospira halophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S1Y OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1S1Y FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
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|GENE= PYP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1053 Halorhodospira halophila])
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HC4:4-HYDROXYCINNAMIC+ACID'>HC4</scene></td></tr>
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}}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1s1y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1s1y OCA], [https://pdbe.org/1s1y PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1s1y RCSB], [https://www.ebi.ac.uk/pdbsum/1s1y PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1s1y ProSAT]</span></td></tr>
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</table>
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'''Photoactivated chromophore conformation in Photoactive Yellow Protein (E46Q mutant) from 10 microseconds to 3 milliseconds'''
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== Function ==
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[https://www.uniprot.org/uniprot/PYP_HALHA PYP_HALHA] Photoactive blue light protein. Probably functions as a photoreceptor for a negative phototaxis response.
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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We use time-resolved crystallography to observe the structural progression of a bacterial blue light photoreceptor throughout its photocycle. Data were collected from 10 ns to 100 ms after photoactivation of the E46Q mutant of photoactive yellow protein. Refinement of transient chromophore conformations shows that the spectroscopically distinct intermediates are formed via progressive disruption of the hydrogen bond network to the chromophore. Although structural change occurs within a few nanoseconds on and around the chromophore, it takes milliseconds for a distinct pattern of tertiary structural change to fully progress through the entire molecule, thus generating the putative signaling state. Remarkably, the coupling between the chromophore conformation and the tertiary structure of this small protein is not tight: there are leads and lags between changes in the conformation of the chromophore and the protein tertiary structure.
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Check<jmol>
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<jmolCheckbox>
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==About this Structure==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/s1/1s1y_consurf.spt"</scriptWhenChecked>
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1S1Y is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Halorhodospira_halophila Halorhodospira halophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S1Y OCA].
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==Reference==
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</jmolCheckbox>
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Chromophore conformation and the evolution of tertiary structural changes in photoactive yellow protein., Anderson S, Srajer V, Pahl R, Rajagopal S, Schotte F, Anfinrud P, Wulff M, Moffat K, Structure. 2004 Jun;12(6):1039-45. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15274923 15274923]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1s1y ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Halorhodospira halophila]]
[[Category: Halorhodospira halophila]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Anderson, S.]]
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[[Category: Anderson S]]
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[[Category: Anfinrud, P.]]
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[[Category: Anfinrud P]]
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[[Category: Moffat, K.]]
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[[Category: Moffat K]]
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[[Category: Pahl, R.]]
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[[Category: Pahl R]]
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[[Category: Rajagopal, S.]]
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[[Category: Rajagopal S]]
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[[Category: Schotte, F.]]
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[[Category: Schotte F]]
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[[Category: Srajer, V.]]
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[[Category: Srajer V]]
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[[Category: Wulff, M.]]
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[[Category: Wulff M]]
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[[Category: HC4]]
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[[Category: room temperature]]
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[[Category: time-resolved]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 23 13:33:24 2008''
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Current revision

Photoactivated chromophore conformation in Photoactive Yellow Protein (E46Q mutant) from 10 microseconds to 3 milliseconds

PDB ID 1s1y

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