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| | ==The solution structure of the nisin-lipid II complex== | | ==The solution structure of the nisin-lipid II complex== |
| - | <StructureSection load='1wco' size='340' side='right' caption='[[1wco]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='1wco' size='340' side='right'caption='[[1wco]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1wco]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lactococcus_lactis Lactococcus lactis] and [http://en.wikipedia.org/wiki/Monarthropalpus_flavus Monarthropalpus flavus]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1uzt 1uzt]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WCO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1WCO FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1wco]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Lactococcus_lactis Lactococcus lactis] and [https://en.wikipedia.org/wiki/Monarthropalpus_flavus Monarthropalpus flavus]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1uzt 1uzt]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WCO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1WCO FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FPP:FARNESYL+DIPHOSPHATE'>FPP</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| - | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=DAL:D-ALANINE'>DAL</scene>, <scene name='pdbligand=DBB:D-ALPHA-AMINOBUTYRIC+ACID'>DBB</scene>, <scene name='pdbligand=DBU:(2Z)-2-AMINOBUT-2-ENOIC+ACID'>DBU</scene>, <scene name='pdbligand=DHA:2-AMINO-ACRYLIC+ACID'>DHA</scene>, <scene name='pdbligand=FGA:GAMMA-D-GLUTAMIC+ACID'>FGA</scene>, <scene name='pdbligand=MUB:N-ACETYLMURAMIC+ACID'>MUB</scene></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DAL:D-ALANINE'>DAL</scene>, <scene name='pdbligand=DBB:D-ALPHA-AMINOBUTYRIC+ACID'>DBB</scene>, <scene name='pdbligand=DBU:(2Z)-2-AMINOBUT-2-ENOIC+ACID'>DBU</scene>, <scene name='pdbligand=DHA:2-AMINO-ACRYLIC+ACID'>DHA</scene>, <scene name='pdbligand=FDF:(2E,6E)-12-FLUORO-11-(FLUOROMETHYL)-3,7-DIMETHYLDODECA-2,6,10-TRIEN-1-YL+TRIHYDROGEN+DIPHOSPHATE'>FDF</scene>, <scene name='pdbligand=FGA:GAMMA-D-GLUTAMIC+ACID'>FGA</scene>, <scene name='pdbligand=MUB:N-ACETYLMURAMIC+ACID'>MUB</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1aj1|1aj1]], [[1mqx|1mqx]], [[1mqy|1mqy]], [[1mqz|1mqz]], [[1qow|1qow]], [[1w9n|1w9n]], [[2dde|2dde]], [[2ktn|2ktn]], [[2kto|2kto]]</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1wco FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wco OCA], [https://pdbe.org/1wco PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1wco RCSB], [https://www.ebi.ac.uk/pdbsum/1wco PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1wco ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1wco FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wco OCA], [http://pdbe.org/1wco PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1wco RCSB], [http://www.ebi.ac.uk/pdbsum/1wco PDBsum]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/LANZ_LACLL LANZ_LACLL]] Lanthionine-containing peptide antibiotic (lantibiotic) active on Gram-positive bacteria. The bactericidal activity of lantibiotics is based on depolarization of energized bacterial cytoplasmic membranes, initiated by the formation of aqueous transmembrane pores. | + | [https://www.uniprot.org/uniprot/LANZ_LACLL LANZ_LACLL] Lanthionine-containing peptide antibiotic (lantibiotic) active on Gram-positive bacteria. The bactericidal activity of lantibiotics is based on depolarization of energized bacterial cytoplasmic membranes, initiated by the formation of aqueous transmembrane pores. |
| - | <div style="background-color:#fffaf0;">
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| - | == Publication Abstract from PubMed ==
| + | |
| - | The emerging antibiotics-resistance problem has underlined the urgent need for novel antimicrobial agents. Lantibiotics (lanthionine-containing antibiotics) are promising candidates to alleviate this problem. Nisin, a member of this family, has a unique pore-forming activity against bacteria. It binds to lipid II, the essential precursor of cell wall synthesis. As a result, the membrane permeabilization activity of nisin is increased by three orders of magnitude. Here we report the solution structure of the complex of nisin and lipid II. The structure shows a novel lipid II-binding motif in which the pyrophosphate moiety of lipid II is primarily coordinated by the N-terminal backbone amides of nisin via intermolecular hydrogen bonds. This cage structure provides a rationale for the conservation of the lanthionine rings among several lipid II-binding lantibiotics. The structure of the pyrophosphate cage offers a template for structure-based design of novel antibiotics.
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| - | | + | |
| - | The nisin-lipid II complex reveals a pyrophosphate cage that provides a blueprint for novel antibiotics.,Hsu ST, Breukink E, Tischenko E, Lutters MA, de Kruijff B, Kaptein R, Bonvin AM, van Nuland NA Nat Struct Mol Biol. 2004 Oct;11(10):963-7. Epub 2004 Sep 12. PMID:15361862<ref>PMID:15361862</ref>
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | ==See Also== |
| - | </div>
| + | *[[Nisin|Nisin]] |
| - | <div class="pdbe-citations 1wco" style="background-color:#fffaf0;"></div>
| + | |
| - | == References == | + | |
| - | <references/>
| + | |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| | [[Category: Lactococcus lactis]] | | [[Category: Lactococcus lactis]] |
| | + | [[Category: Large Structures]] |
| | [[Category: Monarthropalpus flavus]] | | [[Category: Monarthropalpus flavus]] |
| - | [[Category: Bonvin, A M.J J]] | + | [[Category: Bonvin AMJJ]] |
| - | [[Category: Breukink, E]] | + | [[Category: Breukink E]] |
| - | [[Category: Hsu, S T.D]] | + | [[Category: Hsu S-TD]] |
| - | [[Category: Kaptein, R]] | + | [[Category: Kaptein R]] |
| - | [[Category: Kruijff, B De]]
| + | [[Category: Lutters MAG]] |
| - | [[Category: Lutters, M A.G]] | + | [[Category: Tischenko E]] |
| - | [[Category: Nuland, N A.J Van]]
| + | [[Category: De Kruijff B]] |
| - | [[Category: Tischenko, E]] | + | [[Category: Van Nuland NAJ]] |
| - | [[Category: Antimicrobial]] | + | |
| - | [[Category: Bacteriocin]] | + | |
| - | [[Category: Food preservative]]
| + | |
| - | [[Category: Lantibiotic]]
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| - | [[Category: Peptide-antibiotic complex]]
| + | |
| - | [[Category: Pore formation]]
| + | |
| - | [[Category: Pyrophosphate cage]]
| + | |
| - | [[Category: Thioester]]
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