1v39

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[[Image:1v39.gif|left|200px]]
 
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{{Structure
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==DC26 MUTANT OF VACCINIA VIRUS PROTEIN VP39 IN COMPLEX WITH S-ADENOSYLHOMOCYSTEINE AND M7G(5')PPPG==
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|PDB= 1v39 |SIZE=350|CAPTION= <scene name='initialview01'>1v39</scene>, resolution 1.8&Aring;
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<StructureSection load='1v39' size='340' side='right'caption='[[1v39]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene> and <scene name='pdbligand=M7G:7N-METHYL-8-HYDROGUANOSINE-5&#39;-DIPHOSPHATE'>M7G</scene>
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<table><tr><td colspan='2'>[[1v39]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Vaccinia_virus_WR Vaccinia virus WR]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1V39 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1V39 FirstGlance]. <br>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Polynucleotide_adenylyltransferase Polynucleotide adenylyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.19 2.7.7.19]
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=M7G:7N-METHYL-8-HYDROGUANOSINE-5-DIPHOSPHATE'>M7G</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene></td></tr>
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}}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1v39 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1v39 OCA], [https://pdbe.org/1v39 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1v39 RCSB], [https://www.ebi.ac.uk/pdbsum/1v39 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1v39 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/MCE_VACCW MCE_VACCW] Displays methyltransferase, positive regulation of the poly(A) polymerase and transcription elongation activities. Involved in the modification of both mRNA ends and in intermediate and late gene positive transcription elongation. At the mRNAs 5' end, methylates the ribose 2' OH group of the first transcribed nucleotide, thereby producing a 2'-O-methylpurine cap. At the 3' end, functions as a processivity factor which stimulates the activity of the viral poly(A) polymerase VP55 that creates mRNA's poly(A) tail. In the presence of VP39, VP55 does not dissociate from the RNA allowing tail elongation to around 250 adenylates.<ref>PMID:1313572</ref> <ref>PMID:1670500</ref> <ref>PMID:12359447</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/v3/1v39_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1v39 ConSurf].
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<div style="clear:both"></div>
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'''DC26 MUTANT OF VACCINIA VIRUS PROTEIN VP39 IN COMPLEX WITH S-ADENOSYLHOMOCYSTEINE AND M7G(5')PPPG'''
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==See Also==
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*[[Poly(A) polymerase 3D structures|Poly(A) polymerase 3D structures]]
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== References ==
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==Overview==
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<references/>
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The specific binding of N7-methylguanine cap analogues to the RNA methyltransferase VP39 was observed through X-ray crystallography, providing a prototypical structure for a complex between a protein and an mRNA 5' cap.
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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1V39 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Vaccinia_virus Vaccinia virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1V39 OCA].
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[[Category: Vaccinia virus WR]]
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[[Category: Gershon PD]]
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==Reference==
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[[Category: Hodel AE]]
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Specific protein recognition of an mRNA cap through its alkylated base., Hodel AE, Gershon PD, Shi X, Wang SM, Quiocho FA, Nat Struct Biol. 1997 May;4(5):350-4. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9145102 9145102]
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[[Category: Quiocho FA]]
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[[Category: Polynucleotide adenylyltransferase]]
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[[Category: Single protein]]
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[[Category: Vaccinia virus]]
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[[Category: Gershon, P D.]]
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[[Category: Hodel, A E.]]
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[[Category: Quiocho, F A.]]
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[[Category: M7G]]
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[[Category: SAH]]
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[[Category: methyltransferase]]
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[[Category: mrna processing]]
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[[Category: poly(a) polymerase]]
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[[Category: rna cap]]
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[[Category: transcription]]
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[[Category: vaccinia]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 23 13:57:02 2008''
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Current revision

DC26 MUTANT OF VACCINIA VIRUS PROTEIN VP39 IN COMPLEX WITH S-ADENOSYLHOMOCYSTEINE AND M7G(5')PPPG

PDB ID 1v39

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