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| | ==NAB2 N-terminal domain== | | ==NAB2 N-terminal domain== |
| - | <StructureSection load='2jps' size='340' side='right' caption='[[2jps]], [[NMR_Ensembles_of_Models | 45 NMR models]]' scene=''> | + | <StructureSection load='2jps' size='340' side='right'caption='[[2jps]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2jps]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JPS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2JPS FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2jps]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JPS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2JPS FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">NAB2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2jps FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jps OCA], [http://pdbe.org/2jps PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2jps RCSB], [http://www.ebi.ac.uk/pdbsum/2jps PDBsum]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2jps FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jps OCA], [https://pdbe.org/2jps PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2jps RCSB], [https://www.ebi.ac.uk/pdbsum/2jps PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2jps ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/NAB2_YEAST NAB2_YEAST]] This essential protein binds to polyadenylated RNA and single-stranded DNA. It may be involved not only in RNA processing but also in transcription regulation. Believed to associate directly with nascent RNA polymerase II transcripts and remain associated during subsequent nuclear RNA processing reactions. | + | [https://www.uniprot.org/uniprot/NAB2_YEAST NAB2_YEAST] This essential protein binds to polyadenylated RNA and single-stranded DNA. It may be involved not only in RNA processing but also in transcription regulation. Believed to associate directly with nascent RNA polymerase II transcripts and remain associated during subsequent nuclear RNA processing reactions. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Atcc 18824]] | + | [[Category: Large Structures]] |
| - | [[Category: Corbett, A H]] | + | [[Category: Saccharomyces cerevisiae]] |
| - | [[Category: Fasken, M]] | + | [[Category: Corbett AH]] |
| - | [[Category: Grant, R]] | + | [[Category: Fasken M]] |
| - | [[Category: Harreman, M T]] | + | [[Category: Grant R]] |
| - | [[Category: Kelly, S]] | + | [[Category: Harreman MT]] |
| - | [[Category: Marshall, N J]] | + | [[Category: Kelly S]] |
| - | [[Category: Neuhaus, D]] | + | [[Category: Marshall NJ]] |
| - | [[Category: Stewart, M]] | + | [[Category: Neuhaus D]] |
| - | [[Category: Yang, J]] | + | [[Category: Stewart M]] |
| - | [[Category: Protein]]
| + | [[Category: Yang J]] |
| - | [[Category: Protein binding]]
| + | |
| Structural highlights
Function
NAB2_YEAST This essential protein binds to polyadenylated RNA and single-stranded DNA. It may be involved not only in RNA processing but also in transcription regulation. Believed to associate directly with nascent RNA polymerase II transcripts and remain associated during subsequent nuclear RNA processing reactions.
Publication Abstract from PubMed
Nuclear abundant poly(A) RNA-binding protein 2 (Nab2) is an essential yeast heterogeneous nuclear ribonucleoprotein that modulates both mRNA nuclear export and poly(A) tail length. The N-terminal domain of Nab2 (residues 1-97) mediates interactions with both the C-terminal globular domain of the nuclear pore-associated protein, myosin-like protein 1 (Mlp1), and the mRNA export factor, Gfd1. The solution and crystal structures of the Nab2 N-terminal domain show a primarily helical fold that is analogous to the PWI fold found in several other RNA-binding proteins. In contrast to other PWI-containing proteins, we find no evidence that the Nab2 N-terminal domain binds to nucleic acids. Instead, this domain appears to mediate protein:protein interactions that facilitate the nuclear export of mRNA. The Nab2 N-terminal domain has a distinctive hydrophobic patch centered on Phe73, consistent with this region of the surface being a protein:protein interaction site. Engineered mutations within this hydrophobic patch attenuate the interaction with the Mlp1 C-terminal domain but do not alter the interaction with Gfd1, indicating that this patch forms a crucial component of the interface between Nab2 and Mlp1.
Structure of the N-Terminal Mlp1-Binding Domain of the Saccharomyces cerevisiae mRNA-Binding Protein, Nab2.,Grant RP, Marshall NJ, Yang JC, Fasken MB, Kelly SM, Harreman MT, Neuhaus D, Corbett AH, Stewart M J Mol Biol. 2007 Dec 4;. PMID:18190927[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Grant RP, Marshall NJ, Yang JC, Fasken MB, Kelly SM, Harreman MT, Neuhaus D, Corbett AH, Stewart M. Structure of the N-Terminal Mlp1-Binding Domain of the Saccharomyces cerevisiae mRNA-Binding Protein, Nab2. J Mol Biol. 2007 Dec 4;. PMID:18190927 doi:S0022-2836(07)01587-2
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