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1nzg

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==Crystal structure of A-DNA decamer GCGTA(3ME)ACGC, with a modified 5-methyluridine==
==Crystal structure of A-DNA decamer GCGTA(3ME)ACGC, with a modified 5-methyluridine==
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<StructureSection load='1nzg' size='340' side='right' caption='[[1nzg]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
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<StructureSection load='1nzg' size='340' side='right'caption='[[1nzg]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[1nzg]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NZG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1NZG FirstGlance]. <br>
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<table><tr><td colspan='2'>[[1nzg]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NZG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NZG FirstGlance]. <br>
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</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=3ME:2-O-{2-[2-(DIMETHYLAMINO)ETHOXY]ETHYL}-5-METHYLURIDINE+5-(DIHYDROGEN+PHOSPHATE)'>3ME</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1nzg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nzg OCA], [http://pdbe.org/1nzg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1nzg RCSB], [http://www.ebi.ac.uk/pdbsum/1nzg PDBsum]</span></td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=3ME:2-O-{2-[2-(DIMETHYLAMINO)ETHOXY]ETHYL}-5-METHYLURIDINE+5-(DIHYDROGEN+PHOSPHATE)'>3ME</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1nzg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nzg OCA], [https://pdbe.org/1nzg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1nzg RCSB], [https://www.ebi.ac.uk/pdbsum/1nzg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1nzg ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
 
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== Publication Abstract from PubMed ==
 
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[structure: see text] Oligonucleotides with a novel, 2'-O-[2-[2-(N,N-dimethylamino)ethoxy]ethyl] (2'-O-DMAEOE) modification have been synthesized. This modification, a cationic analogue of the 2'-O-(2-methoxyethyl) (2'-O-MOE) modification, exhibits high binding affinity to target RNA (but not to DNA) and exceptional resistance to nuclease degradation. Analysis of the crystal structure of a self-complementary oligonucleotide containing a single 2'-O-DMAEOE modification explains the importance of charge factors and gauche effects on the observed antisense properties. 2'-O-DMAEOE modified oligonucleotides are ideal candidates for antisense drugs.
 
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2'-O-[2-[2-(N,N-dimethylamino)ethoxy]ethyl] modified oligonucleotides: symbiosis of charge interaction factors and stereoelectronic effects.,Prhavc M, Prakash TP, Minasov G, Cook PD, Egli M, Manoharan M Org Lett. 2003 Jun 12;5(12):2017-20. PMID:12790517<ref>PMID:12790517</ref>
 
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 
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</div>
 
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<div class="pdbe-citations 1nzg" style="background-color:#fffaf0;"></div>
 
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== References ==
 
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<references/>
 
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Egli, M]]
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[[Category: Large Structures]]
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[[Category: Manoharan, M]]
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[[Category: Egli M]]
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[[Category: Minasov, G]]
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[[Category: Manoharan M]]
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[[Category: Prakash, T P]]
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[[Category: Minasov G]]
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[[Category: Prhavc, M]]
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[[Category: Prakash TP]]
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[[Category: 2'-modified oligonucleotide]]
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[[Category: Prhavc M]]
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[[Category: A-dna]]
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[[Category: Dna]]
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Crystal structure of A-DNA decamer GCGTA(3ME)ACGC, with a modified 5-methyluridine

PDB ID 1nzg

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