1y27

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[[Image:1y27.gif|left|200px]]
 
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{{Structure
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==G-riboswitch-guanine complex==
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|PDB= 1y27 |SIZE=350|CAPTION= <scene name='initialview01'>1y27</scene>, resolution 2.40&Aring;
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<StructureSection load='1y27' size='340' side='right'caption='[[1y27]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=PO2:HYPOPHOSPHITE'>PO2</scene> and <scene name='pdbligand=G:GUANOSINE-5&#39;-MONOPHOSPHATE'>G</scene>
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<table><tr><td colspan='2'>[[1y27]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. The October 2010 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Riboswitches'' by David Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2010_10 10.2210/rcsb_pdb/mom_2010_10]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y27 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1Y27 FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
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|GENE= XPT ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 Bacillus subtilis])
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CCC:CYTIDINE-5-PHOSPHATE-2,3-CYCLIC+PHOSPHATE'>CCC</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=GUN:GUANINE'>GUN</scene></td></tr>
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}}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1y27 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1y27 OCA], [https://pdbe.org/1y27 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1y27 RCSB], [https://www.ebi.ac.uk/pdbsum/1y27 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1y27 ProSAT]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Metabolite-sensing mRNAs, or "riboswitches," specifically interact with small ligands and direct expression of the genes involved in their metabolism. Riboswitches contain sensing "aptamer" modules, capable of ligand-induced structural changes, and downstream regions, harboring expression-controlling elements. We report the crystal structures of the add A-riboswitch and xpt G-riboswitch aptamer modules that distinguish between bound adenine and guanine with exquisite specificity and modulate expression of two different sets of genes. The riboswitches form tuning fork-like architectures, in which the prongs are held in parallel through hairpin loop interactions, and the internal bubble zippers up to form the purine binding pocket. The bound purines are held by hydrogen bonding interactions involving conserved nucleotides along their entire periphery. Recognition specificity is associated with Watson-Crick pairing of the encapsulated adenine and guanine ligands with uridine and cytosine, respectively.
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'''G-riboswitch-guanine complex'''
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Structural basis for discriminative regulation of gene expression by adenine- and guanine-sensing mRNAs.,Serganov A, Yuan YR, Pikovskaya O, Polonskaia A, Malinina L, Phan AT, Hobartner C, Micura R, Breaker RR, Patel DJ Chem Biol. 2004 Dec;11(12):1729-41. PMID:15610857<ref>PMID:15610857</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1y27" style="background-color:#fffaf0;"></div>
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==Overview==
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==See Also==
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Metabolite-sensing mRNAs, or "riboswitches," specifically interact with small ligands and direct expression of the genes involved in their metabolism. Riboswitches contain sensing "aptamer" modules, capable of ligand-induced structural changes, and downstream regions, harboring expression-controlling elements. We report the crystal structures of the add A-riboswitch and xpt G-riboswitch aptamer modules that distinguish between bound adenine and guanine with exquisite specificity and modulate expression of two different sets of genes. The riboswitches form tuning fork-like architectures, in which the prongs are held in parallel through hairpin loop interactions, and the internal bubble zippers up to form the purine binding pocket. The bound purines are held by hydrogen bonding interactions involving conserved nucleotides along their entire periphery. Recognition specificity is associated with Watson-Crick pairing of the encapsulated adenine and guanine ligands with uridine and cytosine, respectively.
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*[[Riboswitch 3D structures|Riboswitch 3D structures]]
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== References ==
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==About this Structure==
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<references/>
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1Y27 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y27 OCA].
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__TOC__
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</StructureSection>
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==Reference==
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Structural basis for discriminative regulation of gene expression by adenine- and guanine-sensing mRNAs., Serganov A, Yuan YR, Pikovskaya O, Polonskaia A, Malinina L, Phan AT, Hobartner C, Micura R, Breaker RR, Patel DJ, Chem Biol. 2004 Dec;11(12):1729-41. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15610857 15610857]
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[[Category: Bacillus subtilis]]
[[Category: Bacillus subtilis]]
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[[Category: Protein complex]]
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[[Category: Large Structures]]
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[[Category: Patel, D J.]]
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[[Category: RCSB PDB Molecule of the Month]]
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[[Category: Serganov, A.]]
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[[Category: Riboswitches]]
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[[Category: Yuan, Y R.]]
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[[Category: Patel DJ]]
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[[Category: G]]
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[[Category: Serganov A]]
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[[Category: PO2]]
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[[Category: Yuan YR]]
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[[Category: crystal structure]]
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[[Category: g-riboswitch guanine recognition]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 23 14:17:59 2008''
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Current revision

G-riboswitch-guanine complex

PDB ID 1y27

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