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| | ==Isolation and characterization of peptides from Momordica cochinchinensis seeds.== | | ==Isolation and characterization of peptides from Momordica cochinchinensis seeds.== |
| - | <StructureSection load='2knp' size='340' side='right' caption='[[2knp]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='2knp' size='340' side='right'caption='[[2knp]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2knp]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Momordica_cochinchinensis Momordica cochinchinensis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KNP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2KNP FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2knp]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Momordica_cochinchinensis Momordica cochinchinensis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KNP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KNP FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2knp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2knp OCA], [http://pdbe.org/2knp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2knp RCSB], [http://www.ebi.ac.uk/pdbsum/2knp PDBsum]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 20 models</td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2knp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2knp OCA], [https://pdbe.org/2knp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2knp RCSB], [https://www.ebi.ac.uk/pdbsum/2knp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2knp ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/D0VWX1_MOMCO D0VWX1_MOMCO] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| | + | [[Category: Large Structures]] |
| | [[Category: Momordica cochinchinensis]] | | [[Category: Momordica cochinchinensis]] |
| - | [[Category: Chan, L]] | + | [[Category: Chan L]] |
| - | [[Category: Daly, N L]] | + | [[Category: Daly NL]] |
| - | [[Category: Cystine knot motif]]
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| - | [[Category: Cytotoxic]]
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| - | [[Category: Disulfide-rich peptide]]
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| - | [[Category: Mcocc-1]]
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| - | [[Category: Mcocc-2]]
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| - | [[Category: Melanoma cell line]]
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| - | [[Category: Non-hemolytic]]
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| - | [[Category: Seeds extract]]
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| - | [[Category: Unknown function]]
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| Structural highlights
Function
D0VWX1_MOMCO
Publication Abstract from PubMed
The plant Momordica cochinchinensis has traditionally been used in Chinese medicine to treat a variety of illnesses. A range of bioactive molecules have been isolated from this plant, including peptides, which are the focus of this study. Here we report the isolation and characterization of two novel peptides, MCoCC-1 and MCoCC-2, containing 33 and 32 amino acids, respectively, which are toxic against three cancer cell lines. The two peptides are highly homologous to one another, but show no sequence similarity to known peptides. Elucidation of the three-dimensional structure of MCoCC-1 suggests the presence of a cystine knot motif, also found in a family of trypsin inhibitor peptides from this plant. However, unlike its structural counterparts, MCoCC-1 does not inhibit trypsin. MCoCC-1 has a well-defined structure, characterized mainly by a triple-stranded antiparallel beta-sheet, but unlike the majority of cystine knot proteins MCoCC-1 contains a disordered loop presumably as a result of flexibility in a localized region of the molecule. Of the cell lines tested, MCoCC-1 is the most toxic against a human melanoma cell line (MM96L) and is nonhemolytic to human erythrocytes. The role of these peptides within the plant remains to be determined.
Isolation and characterization of peptides from Momordica cochinchinensis seeds.,Chan LY, Wang CK, Major JM, Greenwood KP, Lewis RJ, Craik DJ, Daly NL J Nat Prod. 2009 Aug;72(8):1453-8. PMID:19711988[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Chan LY, Wang CK, Major JM, Greenwood KP, Lewis RJ, Craik DJ, Daly NL. Isolation and characterization of peptides from Momordica cochinchinensis seeds. J Nat Prod. 2009 Aug;72(8):1453-8. PMID:19711988 doi:10.1021/np900174n
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