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| | ==Micelle-bound structure of growth-blocking peptide of the armyworm, Pseudaletia separata== | | ==Micelle-bound structure of growth-blocking peptide of the armyworm, Pseudaletia separata== |
| - | <StructureSection load='2eqt' size='340' side='right' caption='[[2eqt]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='2eqt' size='340' side='right'caption='[[2eqt]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2eqt]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Leucania_separata Leucania separata]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EQT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2EQT FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2eqt]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mythimna_separata Mythimna separata]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EQT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2EQT FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2eqh|2eqh]], [[2eqq|2eqq]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 20 models</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2eqt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2eqt OCA], [http://pdbe.org/2eqt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2eqt RCSB], [http://www.ebi.ac.uk/pdbsum/2eqt PDBsum]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2eqt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2eqt OCA], [https://pdbe.org/2eqt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2eqt RCSB], [https://www.ebi.ac.uk/pdbsum/2eqt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2eqt ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/GBP_MYTSE GBP_MYTSE]] Biogenic peptide that prevents the onset of metamorphosis from larva to pupa. Has repressive activity against juvenile hormone esterase. Induces cell proliferation at low concentrations and inhibits it at high concentrations. Mediates the spreading of plasmatocytes to foreign surfaces. May have a role in the regulation of dopamine concentrations.<ref>PMID:10928276</ref> <ref>PMID:11429413</ref> <ref>PMID:9753606</ref> | + | [https://www.uniprot.org/uniprot/GBP_MYTSE GBP_MYTSE] Biogenic peptide that prevents the onset of metamorphosis from larva to pupa. Has repressive activity against juvenile hormone esterase. Induces cell proliferation at low concentrations and inhibits it at high concentrations. Mediates the spreading of plasmatocytes to foreign surfaces. May have a role in the regulation of dopamine concentrations.<ref>PMID:10928276</ref> <ref>PMID:11429413</ref> <ref>PMID:9753606</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Leucania separata]] | + | [[Category: Large Structures]] |
| - | [[Category: Aizawa, T]] | + | [[Category: Mythimna separata]] |
| - | [[Category: Demura, M]] | + | [[Category: Aizawa T]] |
| - | [[Category: Kamiya, M]] | + | [[Category: Demura M]] |
| - | [[Category: Kawano, K]] | + | [[Category: Kamiya M]] |
| - | [[Category: Kumaki, Y]] | + | [[Category: Kawano K]] |
| - | [[Category: Umetsu, Y]] | + | [[Category: Kumaki Y]] |
| - | [[Category: Cytokine]]
| + | [[Category: Umetsu Y]] |
| - | [[Category: Growth-blocking peptide]]
| + | |
| Structural highlights
Function
GBP_MYTSE Biogenic peptide that prevents the onset of metamorphosis from larva to pupa. Has repressive activity against juvenile hormone esterase. Induces cell proliferation at low concentrations and inhibits it at high concentrations. Mediates the spreading of plasmatocytes to foreign surfaces. May have a role in the regulation of dopamine concentrations.[1] [2] [3]
Publication Abstract from PubMed
Growth-blocking peptide (GBP) is a hormone-like peptide that suppresses the growth of the host armyworm. Although the 23-amino acid GBP (1-23 GBP) is expressed in nonparasitized armyworm plasma, the parasitization by wasp produces the 28-amino acid GBP (1-28 GBP) through an elongation of the C-terminal amino acid sequence. In this study, we characterized the GBP variants, which consist of various lengths of the C-terminal region, by comparing their biological activities and three-dimensional structures. The results of an injection study indicate that 1-28 GBP most strongly suppresses larval growth. NMR analysis shows that these peptides have basically the same tertiary structures and that the extension of the C-terminal region is disordered. However, the C-terminal region of 1-28 GBP undergoes a conformational transition from a random coiled state to an alpha-helical state in the presence of dodecylphosphocholine micelles. This suggests that binding of the C-terminal region would affect larval growth activity.
C-terminal elongation of growth-blocking peptide enhances its biological activity and micelle binding affinity.,Umetsu Y, Aizawa T, Muto K, Yamamoto H, Kamiya M, Kumaki Y, Mizuguchi M, Demura M, Hayakawa Y, Kawano K J Biol Chem. 2009 Oct 23;284(43):29625-34. Epub 2009 Aug 26. PMID:19710009[4]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Hayakawa Y, Ohnishi A, Mizoguchi A, Yamashika C. Distribution of growth-blocking peptide in the insect central nervous tissue. Cell Tissue Res. 2000 Jun;300(3):459-64. PMID:10928276
- ↑ Aizawa T, Hayakawa Y, Ohnishi A, Fujitani N, Clark KD, Strand MR, Miura K, Koganesawa N, Kumaki Y, Demura M, Nitta K, Kawano K. Structure and activity of the insect cytokine growth-blocking peptide. Essential regions for mitogenic and hemocyte-stimulating activities are separate. J Biol Chem. 2001 Aug 24;276(34):31813-8. Epub 2001 Jun 27. PMID:11429413 doi:http://dx.doi.org/10.1074/jbc.M105251200
- ↑ Hayakawa Y, Ohnishi A. Cell growth activity of growth-blocking peptide. Biochem Biophys Res Commun. 1998 Sep 18;250(2):194-9. PMID:9753606 doi:http://dx.doi.org/10.1006/bbrc.1998.8959
- ↑ Umetsu Y, Aizawa T, Muto K, Yamamoto H, Kamiya M, Kumaki Y, Mizuguchi M, Demura M, Hayakawa Y, Kawano K. C-terminal elongation of growth-blocking peptide enhances its biological activity and micelle binding affinity. J Biol Chem. 2009 Oct 23;284(43):29625-34. Epub 2009 Aug 26. PMID:19710009 doi:10.1074/jbc.M109.011148
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