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2des

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[[Image:2des.jpg|left|200px]]
 
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{{Structure
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==INTERACTIONS BETWEEN MORPHOLINYL ANTHRACYCLINES AND DNA: THE CRYSTAL STRUCTURE OF A MORPHOLINO DOXORUBICIN BOUND TO D(CGTACG)==
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|PDB= 2des |SIZE=350|CAPTION= <scene name='initialview01'>2des</scene>, resolution 1.500&Aring;
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<StructureSection load='2des' size='340' side='right'caption='[[2des]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=DMM:3&#39;-DESAMINO-3&#39;-(2-METHOXY-4-MORPHOLINYL)-DOXORUBICIN'>DMM</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> and <scene name='pdbligand=NA:SODIUM ION'>NA</scene>
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<table><tr><td colspan='2'>[[2des]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DES OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2DES FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DMM:3-DESAMINO-3-(2-METHOXY-4-MORPHOLINYL)-DOXORUBICIN'>DMM</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
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}}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2des FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2des OCA], [https://pdbe.org/2des PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2des RCSB], [https://www.ebi.ac.uk/pdbsum/2des PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2des ProSAT]</span></td></tr>
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</table>
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'''INTERACTIONS BETWEEN MORPHOLINYL ANTHRACYCLINES AND DNA: THE CRYSTAL STRUCTURE OF A MORPHOLINO DOXORUBICIN BOUND TO D(CGTACG)'''
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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==Overview==
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[[Category: Bachechi F]]
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Anthracycline antibiotics daunomycin and adriamycin are among the most widely used in cancer chemotherapy and DNA is believed to be the primary target of their biological action. The crystal structure of a morpholino derivative of adriamycin bound to the DNA hexamer d(CGTACG) has been determined at 1.5 A resolution. The complex crystallizes in space group P1 with unit cell dimensions a = 18.01 A, b = 18.83 A, c = 27.65 A, alpha = 92.6 degrees, beta = 100.5 degrees, gamma = 94.9 degrees and there are two drug molecules bound per duplex. Morpholino derivatives differ greatly from their parent compounds in their biological and pharmacological properties. Structural comparison of this complex with the series of previously reported anthracycline-DNA complexes offers an opportunity for studying relationships between structure and function. The anthracycline chromophore intercalates at the CpG step and DNA distortions from a B-type conformation are similar to those observed in the other DNA-anthracycline complexes. Interactions between drug and DNA show no differences at the intercalation site, while in the minor groove they are significantly affected by the presence of the bulky morpholinyl moiety on the anthracycline amino sugar. The binding site involves four base-pairs and the absence of a positive charge on the amino sugar appears to influence the hydration pattern on both grooves. The two halves of the duplex are symmetrically related by a non-crystallographic 2-fold axis but they are not equivalent. In one half, one magnesium cluster bridges both drug and DNA, further stabilizing the complex.
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[[Category: Capobianco ML]]
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[[Category: Cirilli M]]
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==About this Structure==
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[[Category: Colonna FP]]
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2DES is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DES OCA].
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[[Category: Ughetto G]]
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==Reference==
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Interactions between morpholinyl anthracyclines and DNA. The crystal structure of a morpholino doxorubicin bound to d(CGTACG)., Cirilli M, Bachechi F, Ughetto G, Colonna FP, Capobianco ML, J Mol Biol. 1993 Apr 5;230(3):878-89. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8478940 8478940]
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[[Category: Protein complex]]
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[[Category: Bachechi, F.]]
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[[Category: Capobianco, M L.]]
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[[Category: Cirilli, M.]]
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[[Category: Colonna, F P.]]
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[[Category: Ughetto, G.]]
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[[Category: DMM]]
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[[Category: MG]]
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[[Category: NA]]
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[[Category: complexed with drug]]
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[[Category: double helix]]
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[[Category: right handed dna]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 23 14:56:11 2008''
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Current revision

INTERACTIONS BETWEEN MORPHOLINYL ANTHRACYCLINES AND DNA: THE CRYSTAL STRUCTURE OF A MORPHOLINO DOXORUBICIN BOUND TO D(CGTACG)

PDB ID 2des

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