2mez

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==Flexible anchoring of archaeal MBF1 on ribosomes suggests role as recruitment factor==
==Flexible anchoring of archaeal MBF1 on ribosomes suggests role as recruitment factor==
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<StructureSection load='2mez' size='340' side='right' caption='[[2mez]], [[NMR_Ensembles_of_Models | 16 NMR models]]' scene=''>
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<StructureSection load='2mez' size='340' side='right'caption='[[2mez]]' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[2mez]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MEZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2MEZ FirstGlance]. <br>
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<table><tr><td colspan='2'>[[2mez]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharolobus_solfataricus_P2 Saccharolobus solfataricus P2]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MEZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MEZ FirstGlance]. <br>
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</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2mez FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mez OCA], [http://pdbe.org/2mez PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2mez RCSB], [http://www.ebi.ac.uk/pdbsum/2mez PDBsum]</span></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2mez FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mez OCA], [https://pdbe.org/2mez PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2mez RCSB], [https://www.ebi.ac.uk/pdbsum/2mez PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2mez ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/Q980M2_SACS2 Q980M2_SACS2]
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Multi-Protein Bridging factor 1 (MBF1) is a protein containing a conserved helix-turn-helix domain in both eukaryotes and archaea. Eukaryotic MBF1 has been reported to function as a transcriptional co-activator that physically bridges transcription regulators with the core transcription initiation machinery of RNA polymerase II. In addition, MBF1 has been found to be associated with poly-adenylated mRNA in yeast as well as mammalian cells. Archaeal MBF1 (aMBF1) is very well conserved among most archaeal lineages, however, its function has remained elusive thus far. To address this we have conducted a molecular characterization of this aMBF1. Affinity purification of interacting proteins indicates that aMBF1 binds to ribosomal subunits. On sucrose density gradients, aMBF1 co-fractionates with free 30S ribosomal subunits as well as with 70S ribosomes engaged in translation. Binding of aMBF1 to ribosomes does not inhibit translation. Using NMR spectroscopy, we show that aMBF1 contains a long intrinsically disordered linker connecting the predicted N-terminal Zn-ribbon domain with the C-terminal helix-turn-helix (HTH) domain. The HTH domain, which is conserved in all archaeal and eukaryotic MBF1 homologues, is directly involved in the association of aMBF1 with ribosomes. The disordered linker of the ribosome-bound aMBF1 provides the N-terminal domain with high flexibility in the aMBF1-ribosome complex. Overall, our data suggest a role for aMBF1 in the archaeal translation process.
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Archaeal MBF1 binds to 30S and 70S ribosomes via its helix-turn-helix domain.,Blombach F, Launay H, Snijders AP, Zorraquino V, Wu H, de Koning B, Brouns SJ, Ettema T, Camilloni C, Cavalli A, Vendruscolo M, Dickman MJ, Cabrita LD, La Teana A, Benelli D, Londei P, Christodoulou J, van der Oost J Biochem J. 2014 May 14. PMID:24825021<ref>PMID:24825021</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2mez" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Blombarch, F]]
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[[Category: Large Structures]]
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[[Category: Camilloni, C]]
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[[Category: Saccharolobus solfataricus P2]]
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[[Category: Christodoulou, J]]
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[[Category: Blombarch F]]
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[[Category: Launay, H]]
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[[Category: Camilloni C]]
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[[Category: Oost, J van des]]
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[[Category: Christodoulou J]]
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[[Category: Vendruscolo, M]]
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[[Category: Launay H]]
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[[Category: Helix-turn-helix domain]]
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[[Category: Vendruscolo M]]
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[[Category: Multiple binding factor mbf1]]
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[[Category: Van des Oost J]]
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[[Category: Rna binding protein]]
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Current revision

Flexible anchoring of archaeal MBF1 on ribosomes suggests role as recruitment factor

PDB ID 2mez

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