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| - | [[Image:2hlb.gif|left|200px]]
| + | <table cellpadding='5'><tr><td style='background-color: yellow;border:2px solid black;font-size:150%;'> |
| - | | + | NOTE: This structure was removed. For additional information see '''2012, January:''' at [[Retractions and Fraud]].</td></tr></table> |
| - | {{Structure
| + | REMOVED: The PDB entry 2hlb was removed. |
| - | |PDB= 2hlb |SIZE=350|CAPTION= <scene name='initialview01'>2hlb</scene>, resolution 2.2Å
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| - | |SITE=
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| - | |LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> and <scene name='pdbligand=GDP:GUANOSINE-5'-DIPHOSPHATE'>GDP</scene>
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| - | |ACTIVITY=
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| - | |GENE= GNAI1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
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| - | }}
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| - | '''A Structural Basis for Nucleotide Exchange on G-alpha-i Subunits and Receptor Coupling Specificity''' | + | |
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| - | ==Overview==
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| - | Heterotrimeric G proteins are molecular switches that relay information intracellularly in response to various extracellular signals. How ligand-activated G protein-coupled receptors act at a distance to exert exchange activity on the Galpha nucleotide binding pocket is poorly understood. Here we describe the synergistic action of two peptides: one from the third intracellular loop of the D2 dopamine receptor (D2N), and a second, Galpha.GDP-binding peptide (KB-752) that mimics the proposed role of Gbetagamma in receptor-promoted nucleotide exchange. The structure of both peptides in complex with Galpha(i1) suggests that conformational changes in the beta3/alpha2 loop and beta6 strand act in concert for efficient nucleotide exchange. Two key residues in the alpha4 helix were found to define a receptor/Galpha(i) coupling specificity determinant.
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| - | ==Disease==
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| - | Known disease associated with this structure: Dystonia, myoclonic OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=126450 126450]]
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| - | ==About this Structure==
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| - | 2HLB is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HLB OCA].
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| - | ==Reference==
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| - | Structural basis for nucleotide exchange on G alpha i subunits and receptor coupling specificity., Johnston CA, Siderovski DP, Proc Natl Acad Sci U S A. 2007 Feb 6;104(6):2001-6. Epub 2007 Jan 30. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17264214 17264214]
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| - | [[Category: Homo sapiens]]
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| - | [[Category: Protein complex]]
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| - | [[Category: Johnston, C A.]]
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| - | [[Category: Siderovski, D P.]]
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| - | [[Category: Watts, V J.]]
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| - | [[Category: GDP]]
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| - | [[Category: SO4]]
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| - | [[Category: protein:peptide complex]]
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| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 23 15:16:01 2008''
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