2kgf

From Proteopedia

(Difference between revisions)

Current revision

N-terminal domain of capsid protein from the Mason-Pfizer monkey virus

Structural highlights

2kgf is a 1 chain structure with sequence from Mason-Pfizer monkey virus. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Solution NMR
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

GAG_MPMV p10 is the matrix protein. P14 is the nucleocapsid protein. p27 is the capsid protein.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The high-resolution structure of the N-terminal domain (NTD) of the retroviral capsid protein (CA) of Mason-Pfizer monkey virus (M-PMV), a member of the betaretrovirus family, has been determined by NMR. The M-PMV NTD CA structure is similar to the other retroviral capsid structures and is characterized by a six alpha-helix bundle and an N-terminal beta-hairpin, stabilized by an interaction of highly conserved residues, Pro1 and Asp57. Since the role of the beta-hairpin has been shown to be critical for formation of infectious viral core, we also investigated the functional role of M-PMV beta-hairpin in two mutants (i.e., DeltaP1NTDCA and D57ANTDCA) where the salt bridge stabilizing the wild-type structure was disrupted. NMR data obtained for these mutants were compared with those obtained for the wild type. The main structural changes were observed within the beta-hairpin structure; within helices 2, 3, and 5; and in the loop connecting helices 2 and 3. This observation is supported by biochemical data showing different cleavage patterns of the wild-type and the mutated capsid-nucleocapsid fusion protein (CANC) by M-PMV protease. Despite these structural changes, the mutants with disrupted salt bridge are still able to assemble into immature, spherical particles. This confirms that the mutual interaction and topology within the beta-hairpin and helix 3 might correlate with the changes in interaction between immature and mature lattices.

NMR structure of the N-terminal domain of capsid protein from the mason-pfizer monkey virus.,Macek P, Chmelik J, Krizova I, Kaderavek P, Padrta P, Zidek L, Wildova M, Hadravova R, Chaloupkova R, Pichova I, Ruml T, Rumlova M, Sklenar V J Mol Biol. 2009 Sep 11;392(1):100-14. Epub 2009 Jun 13. PMID:19527730^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Macek P, Chmelik J, Krizova I, Kaderavek P, Padrta P, Zidek L, Wildova M, Hadravova R, Chaloupkova R, Pichova I, Ruml T, Rumlova M, Sklenar V. NMR structure of the N-terminal domain of capsid protein from the mason-pfizer monkey virus. J Mol Biol. 2009 Sep 11;392(1):100-14. Epub 2009 Jun 13. PMID:19527730 doi:10.1016/j.jmb.2009.06.029

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2kgf"

@@ Line 1: / Line 1: @@
 ==N-terminal domain of capsid protein from the Mason-Pfizer monkey virus==
-<StructureSection load='2kgf' size='340' side='right' caption='[[2kgf]], [[NMR_Ensembles_of_Models | 50 NMR models]]' scene=''>
+<StructureSection load='2kgf' size='340' side='right'caption='[[2kgf]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2kgf]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mpmv Mpmv]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KGF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2KGF FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2kgf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mason-Pfizer_monkey_virus Mason-Pfizer monkey virus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KGF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KGF FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2kgf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kgf OCA], [http://pdbe.org/2kgf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2kgf RCSB], [http://www.ebi.ac.uk/pdbsum/2kgf PDBsum]</span></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2kgf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kgf OCA], [https://pdbe.org/2kgf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2kgf RCSB], [https://www.ebi.ac.uk/pdbsum/2kgf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2kgf ProSAT]</span></td></tr>
 </table>
-{{Large structure}}
 == Function ==
-[[http://www.uniprot.org/uniprot/GAG_MPMV GAG_MPMV]] p10 is the matrix protein.  P14 is the nucleocapsid protein.  p27 is the capsid protein.
+[https://www.uniprot.org/uniprot/GAG_MPMV GAG_MPMV] p10 is the matrix protein.  P14 is the nucleocapsid protein.  p27 is the capsid protein.
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
 Check<jmol>
   <jmolCheckbox>
-    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kg/2kgf_consurf.spt"</scriptWhenChecked>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kg/2kgf_consurf.spt"</scriptWhenChecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
   </jmolCheckbox>
-</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2kgf ConSurf].
 <div style="clear:both"></div>
 <div style="background-color:#fffaf0;">
@@ Line 29: / Line 30: @@
 ==See Also==
-*[[Virus coat protein|Virus coat protein]]
+*[[Virus coat proteins 3D structures|Virus coat proteins 3D structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Mpmv]]
+[[Category: Large Structures]]
-[[Category: Chmelik, J]]
+[[Category: Mason-Pfizer monkey virus]]
-[[Category: Kaderavek, P]]
+[[Category: Chmelik J]]
-[[Category: Macek, P]]
+[[Category: Kaderavek P]]
-[[Category: Padrta, P]]
+[[Category: Macek P]]
-[[Category: Pichova, I]]
+[[Category: Padrta P]]
-[[Category: Ruml, T]]
+[[Category: Pichova I]]
-[[Category: Rumlova, M]]
+[[Category: Ruml T]]
-[[Category: Sklenar, V]]
+[[Category: Rumlova M]]
-[[Category: Zidek, L]]
+[[Category: Sklenar V]]
-[[Category: Capsid protein]]
+[[Category: Zidek L]]
-[[Category: Retrovirus capsid protein]]
-[[Category: Viral protein]]

2kgf

From Proteopedia

Current revision

N-terminal domain of capsid protein from the Mason-Pfizer monkey virus

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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