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| - | ==SOLUTION STRUCTURE OF SELENOCOSMIA HUWENA LECTIN-I(SHL-I) BY 2D-NMR== | + | |
| - | <StructureSection load='1qk7' size='340' side='right' caption='[[1qk7]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | ==Solution structure of Selenocosmia huwena lectin-I(SHL-I) by 2D-NMR== |
| | + | <StructureSection load='1qk7' size='340' side='right'caption='[[1qk7]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1qk7]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Haplopelma_schmidti Haplopelma schmidti]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QK7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1QK7 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1qk7]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Cyriopagopus_schmidti Cyriopagopus schmidti]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QK7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1QK7 FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1qk7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qk7 OCA], [http://pdbe.org/1qk7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1qk7 RCSB], [http://www.ebi.ac.uk/pdbsum/1qk7 PDBsum]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 20 models</td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1qk7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qk7 OCA], [https://pdbe.org/1qk7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1qk7 RCSB], [https://www.ebi.ac.uk/pdbsum/1qk7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1qk7 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/TXLA1_CYRSC TXLA1_CYRSC] Agglutinates human and mice erythrocytes. This activity can be specifically inhibited by mannosamine. This lectin shows very low toxicity in both mammals and insects.<ref>PMID:8588212</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Haplopelma schmidti]] | + | [[Category: Cyriopagopus schmidti]] |
| - | [[Category: Gu, X]] | + | [[Category: Large Structures]] |
| - | [[Category: Liang, S]] | + | [[Category: Gu X]] |
| - | [[Category: Lu, S]] | + | [[Category: Liang S]] |
| - | [[Category: Cystine knot]] | + | [[Category: Lu S]] |
| - | [[Category: Lectin]]
| + | |
| - | [[Category: Selenocosmia huwena]]
| + | |
| - | [[Category: Shl-i]]
| + | |
| Structural highlights
Function
TXLA1_CYRSC Agglutinates human and mice erythrocytes. This activity can be specifically inhibited by mannosamine. This lectin shows very low toxicity in both mammals and insects.[1]
Publication Abstract from PubMed
The three-dimensional structure of native SHL-I, a lectin from the venom of the Chinese bird spider Selenocosmia huwena, has been determined from two-dimensional 1H NMR spectroscopy recorded at 500 and 600 MHz. The best 10 structures have NOE violation <0.3 A, dihedral violation <2 deg, and average root-mean-square differences of 0.85 + 0.06 A over backbone atoms. The structure consists of a three-stranded antiparallel beta-sheet and three turns. The three disulfide bridges and three-stranded antiparallel beta-sheet form a inhibitor cystine knot motif which is adopted by several other small proteins, such as huwentoxin-I, omega-conotoxin, and gurmarin. The C-terminal fragment from Leu28 to Trp32 adopts two sets of conformations corresponding to the cis and trans conformations of Pro31. The structure of SHL-I also has high similarity with that of the N-terminus of hevein, a lectin from rubber-tree latex.
Three-dimensional structure of Selenocosmia huwena lectin-I (SHL-I) from the venom of the spider Selenocosmia huwena by 2D-NMR.,Lu S, Liang S, Gu X J Protein Chem. 1999 Jul;18(5):609-17. PMID:10524779[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Liang SP, Pan X. A lectin-like peptide isolated from the venom of the Chinese bird spider Selenocosmia huwena. Toxicon. 1995 Jul;33(7):875-82. PMID:8588212 doi:10.1016/0041-0101(95)00033-i
- ↑ Lu S, Liang S, Gu X. Three-dimensional structure of Selenocosmia huwena lectin-I (SHL-I) from the venom of the spider Selenocosmia huwena by 2D-NMR. J Protein Chem. 1999 Jul;18(5):609-17. PMID:10524779
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