|
|
| (5 intermediate revisions not shown.) |
| Line 1: |
Line 1: |
| | + | |
| | ==NUCLEAR MAGNETIC RESONANCE SOLUTION STRUCTURE OF THE PHEROMONE ER-10 FROM THE CILIATED PROTOZOAN EUPLOTES RAIKOVI== | | ==NUCLEAR MAGNETIC RESONANCE SOLUTION STRUCTURE OF THE PHEROMONE ER-10 FROM THE CILIATED PROTOZOAN EUPLOTES RAIKOVI== |
| - | <StructureSection load='1erp' size='340' side='right' caption='[[1erp]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='1erp' size='340' side='right'caption='[[1erp]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1erp]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Eupra Eupra]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ERP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ERP FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1erp]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Euplotes_raikovi Euplotes raikovi]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ERP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ERP FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1erp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1erp OCA], [http://pdbe.org/1erp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1erp RCSB], [http://www.ebi.ac.uk/pdbsum/1erp PDBsum]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 20 models</td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1erp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1erp OCA], [https://pdbe.org/1erp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1erp RCSB], [https://www.ebi.ac.uk/pdbsum/1erp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1erp ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/MER10_EUPRA MER10_EUPRA]] Mating ciliate pheromones (or gamones) are diffusible extracellular communication signals that distinguish different intraspecific classes of cells commonly referred to as 'mating types'. They prepare the latter for conjugation by changing their cell surface properties. | + | [https://www.uniprot.org/uniprot/MER10_EUPRA MER10_EUPRA] Mating ciliate pheromones (or gamones) are diffusible extracellular communication signals that distinguish different intraspecific classes of cells commonly referred to as 'mating types'. They prepare the latter for conjugation by changing their cell surface properties. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
| Line 20: |
Line 22: |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Eupra]] | + | [[Category: Euplotes raikovi]] |
| - | [[Category: Bradshaw, R]] | + | [[Category: Large Structures]] |
| - | [[Category: Brown, L R]] | + | [[Category: Bradshaw R]] |
| - | [[Category: Luporini, P]] | + | [[Category: Brown LR]] |
| - | [[Category: Mronga, S]] | + | [[Category: Luporini P]] |
| - | [[Category: Ortenzi, C]] | + | [[Category: Mronga S]] |
| - | [[Category: Wuthrich, K]] | + | [[Category: Ortenzi C]] |
| - | [[Category: Pheromone]]
| + | [[Category: Wuthrich K]] |
| Structural highlights
Function
MER10_EUPRA Mating ciliate pheromones (or gamones) are diffusible extracellular communication signals that distinguish different intraspecific classes of cells commonly referred to as 'mating types'. They prepare the latter for conjugation by changing their cell surface properties.
Publication Abstract from PubMed
The three-dimensional structure in solution of the pheromone Er-10 from the ciliated protozoan Euplotes raikovi has been determined by nuclear magnetic resonance spectroscopy. The structure of this 38-residue protein was obtained from 384 nuclear Overhauser enhancement distance constraints and 78 dihedral angle constraints using the distance geometry program DIANA for the structure calculation and the program AMBER for energy minimization. For a group of 20 conformers used to characterize the solution conformation, the average root-mean-square distance calculated for the backbone heavy atoms relative to the mean structure was 0.33 A. The structure includes three short helices of residues 2 to 8, 12 to 19 and 24 to 33, and a turn in the carboxy-terminal region of residues 34 to 38. These structural elements are held together by three disulfide bridges. The structure is quite stable relative to heat denaturation, since at both pH 4.6 and pH 6.0 only minor changes in the circular dichroism and nuclear magnetic resonance spectra were observed over the temperature range 20 to 80 degrees C. The surface of the Er-10 structure shows an asymmetric charge distribution that results in a predominantly apolar surface on one side of the molecule. There is also a deep cleft in the structure with an asymmetric distribution of charged and apolar residues on the two walls. These surface features may be important for the homologous (autocrine) and heterologous binding of the pheromone to receptors.
Nuclear magnetic resonance solution structure of the pheromone Er-10 from the ciliated protozoan Euplotes raikovi.,Brown LR, Mronga S, Bradshaw RA, Ortenzi C, Luporini P, Wuthrich K J Mol Biol. 1993 Jun 5;231(3):800-16. PMID:8515452[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Brown LR, Mronga S, Bradshaw RA, Ortenzi C, Luporini P, Wuthrich K. Nuclear magnetic resonance solution structure of the pheromone Er-10 from the ciliated protozoan Euplotes raikovi. J Mol Biol. 1993 Jun 5;231(3):800-16. PMID:8515452 doi:http://dx.doi.org/S0022-2836(83)71327-6
|
