1cvx

<StructureSection load='1cvx' size='340' side='right' caption='[[1cvx]], [[Resolution|resolution]] 2.27&Aring;' scene=''>

<table><tr><td colspan='2'>[[1cvx]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CVX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CVX FirstGlance]. <br>

</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HP2:HYDROXYPYRROLE-IMIDAZOLE-PYRROLE+POLYAMIDE'>HP2</scene></td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cvx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cvx OCA], [http://pdbe.org/1cvx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1cvx RCSB], [http://www.ebi.ac.uk/pdbsum/1cvx PDBsum]</span></td></tr>

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== Publication Abstract from PubMed ==

Synthetic polyamides composed of three types of aromatic amino acids, N-methylimidazole (Im), N-methylpyrrole (Py) and N-methyl-3-hydroxypyrrole (Hp) bind specific DNA sequences as antiparallel dimers in the minor groove. The side-by-side pairings of aromatic rings in the dimer afford a general recognition code that allows all four base-pairs to be distinguished. To examine the structural consequences of changing the DNA sequence context on T.A recognition by Hp/Py pairs in the minor groove, crystal structures of polyamide dimers (ImPyHpPy)(2) and the pyrrole counterpart (ImPyPyPy)(2) bound to the six base-pair target site 5'-AGATCT-3' in a ten base-pair oligonucleotide have been determined to a resolution of 2.27 and 2.15 A, respectively. The structures demonstrate that the principles of Hp/Py recognition of T.A are consistent between different sequence contexts. However, a general structural explanation for the non-additive reduction in binding affinity due to introduction of the hydroxyl group is less clear. Comparison with other polyamide-DNA cocrystal structures reveals structural themes and differences that may relate to sequence preference.

Structural effects of DNA sequence on T.A recognition by hydroxypyrrole/pyrrole pairs in the minor groove.,Kielkopf CL, Bremer RE, White S, Szewczyk JW, Turner JM, Baird EE, Dervan PB, Rees DC J Mol Biol. 2000 Jan 21;295(3):557-67. PMID:10623546<ref>PMID:10623546</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>

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<div class="pdbe-citations 1cvx" style="background-color:#fffaf0;"></div>

== References ==

<references/>

[[Category: Baird, E E]]

[[Category: Bremer, R E]]

[[Category: Dervan, P B]]

[[Category: Kielkopf, C L]]

[[Category: Rees, D C]]

[[Category: White, S]]

[[Category: Ta recognition]]