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| | ==NMR structure of Spinach Thylakoid Soluble Phosphoprotein of 9 kDa in SDS Micelles== | | ==NMR structure of Spinach Thylakoid Soluble Phosphoprotein of 9 kDa in SDS Micelles== |
| - | <StructureSection load='2fft' size='340' side='right' caption='[[2fft]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='2fft' size='340' side='right'caption='[[2fft]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2fft]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Spiol Spiol]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FFT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2FFT FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2fft]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Spinacia_oleracea Spinacia oleracea]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FFT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FFT FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">TSP9 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3562 SPIOL])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2fft FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fft OCA], [http://pdbe.org/2fft PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2fft RCSB], [http://www.ebi.ac.uk/pdbsum/2fft PDBsum], [http://www.topsan.org/Proteins/CESG/2fft TOPSAN]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2fft FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fft OCA], [https://pdbe.org/2fft PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2fft RCSB], [https://www.ebi.ac.uk/pdbsum/2fft PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2fft ProSAT], [https://www.topsan.org/Proteins/CESG/2fft TOPSAN]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q8GT36_SPIOL Q8GT36_SPIOL] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Spiol]] | + | [[Category: Large Structures]] |
| - | [[Category: Structural genomic]] | + | [[Category: Spinacia oleracea]] |
| - | [[Category: Carlberg, I]] | + | [[Category: Carlberg I]] |
| - | [[Category: Lee, M S]] | + | [[Category: Lee MS]] |
| - | [[Category: Markley, J L]] | + | [[Category: Markley JL]] |
| - | [[Category: Song, J]] | + | [[Category: Song J]] |
| - | [[Category: Cesg]]
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| - | [[Category: Plant protein]]
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| - | [[Category: PSI, Protein structure initiative]]
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| - | [[Category: Thylakoid soluble phosphoprotein]]
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| - | [[Category: Tsp9]]
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| Structural highlights
Function
Q8GT36_SPIOL
Publication Abstract from PubMed
Thylakoid soluble phosphoprotein of 9 kDa (TSP9) has been identified as a plant-specific protein in the photosynthetic thylakoid membrane (Carlberg et al. (2003) Proc. Natl. Acad. Sci. 100, 757-762). Nonphosphorylated TSP9 is associated with the membrane, whereas, after light-induced phosphorylation, a fraction of the phosphorylated TSP9 is released into the aqueous stroma. By NMR spectroscopy, we have determined the structural features of nonphosphorylated TSP9 both in aqueous solution and in membrane mimetic micelles. The results show that both wild type nonphosphorylated TSP9 and a triple-mutant (T46E + T53E + T60E) mimic of the triphosphorylated form of TSP9 are disordered under aqueous conditions, but adopt an ordered conformation in the presence of detergent micelles. The micelle-induced structural features, which are similar in micelles either of SDS or dodecylphosphocholine (DPC), consist of an N-terminal alpha-helix, which may represent the primary site of interaction between TSP9 and binding partners, and a less structured helical turn near the C-terminus. These structured elements contain mainly hydrophobic residues. NMR relaxation data for nonphosphorylated TSP9 in SDS micelles indicated that the molecule is highly flexible with the highest order in the N-terminal alpha-helix. Intermolecular NOE signals, as well as spin probe-induced broadening of NMR signals, demonstrated that the SDS micelles contact both the structured and a portion of the unstructured regions of TSP9, in particular, those containing the three phosphorylation sites (T46, T53, and T60). This interaction may explain the selective dissociation of phosphorylated TSP9 from the membrane. Our study presents a structural model for the role played by the structured and unstructured regions of TSP9 in its membrane association and biological function.
Micelle-induced folding of spinach thylakoid soluble phosphoprotein of 9 kDa and its functional implications.,Song J, Lee MS, Carlberg I, Vener AV, Markley JL Biochemistry. 2006 Dec 26;45(51):15633-43. Epub 2006 Dec 8. PMID:17176085[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Song J, Lee MS, Carlberg I, Vener AV, Markley JL. Micelle-induced folding of spinach thylakoid soluble phosphoprotein of 9 kDa and its functional implications. Biochemistry. 2006 Dec 26;45(51):15633-43. Epub 2006 Dec 8. PMID:17176085 doi:10.1021/bi062148m
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