3b5z

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[[Image:3b5z.jpg|left|200px]]
 
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{{Structure
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==Crystal Structure of MsbA from Salmonella typhimurium with ADP Vanadate==
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|PDB= 3b5z |SIZE=350|CAPTION= <scene name='initialview01'>3b5z</scene>, resolution 4.200&Aring;
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<StructureSection load='3b5z' size='340' side='right'caption='[[3b5z]], [[Resolution|resolution]] 4.20&Aring;' scene=''>
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|SITE= <scene name='pdbsite=AC1:Adp+Binding+Site+For+Residue+A+5001'>AC1</scene>, <scene name='pdbsite=AC2:Adp+Binding+Site+For+Residue+B+5002'>AC2</scene>, <scene name='pdbsite=AC3:Adp+Binding+Site+For+Residue+C+5003'>AC3</scene> and <scene name='pdbsite=AC4:Adp+Binding+Site+For+Residue+D+5004'>AC4</scene>
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=ADP:ADENOSINE-5&#39;-DIPHOSPHATE'>ADP</scene>
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<table><tr><td colspan='2'>[[3b5z]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_Typhimurium Salmonella enterica subsp. enterica serovar Typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B5Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3B5Z FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 4.2&#8491;</td></tr>
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|GENE= msbA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=602 Salmonella typhimurium])
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=VO4:VANADATE+ION'>VO4</scene></td></tr>
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}}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3b5z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3b5z OCA], [https://pdbe.org/3b5z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3b5z RCSB], [https://www.ebi.ac.uk/pdbsum/3b5z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3b5z ProSAT]</span></td></tr>
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</table>
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'''Crystal Structure of MsbA from Salmonella typhimurium with ADP Vanadate'''
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== Function ==
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[https://www.uniprot.org/uniprot/MSBA_SALTY MSBA_SALTY] Involved in lipid A export and possibly also in glycerophospholipid export and for biogenesis of the outer membrane. Transmembrane domains (TMD) form a pore in the inner membrane and the ATP-binding domain (NBD) is responsible for energy generation (By similarity).
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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ATP-binding cassette (ABC) transporters are integral membrane proteins that translocate a wide variety of substrates across cellular membranes and are conserved from bacteria to humans. Here we compare four x-ray structures of the bacterial ABC lipid flippase, MsbA, trapped in different conformations, two nucleotide-bound structures and two in the absence of nucleotide. Comparison of the nucleotide-free conformations of MsbA reveals a flexible hinge formed by extracellular loops 2 and 3. This hinge allows the nucleotide-binding domains to disassociate while the ATP-binding half sites remain facing each other. The binding of the nucleotide causes a packing rearrangement of the transmembrane helices and changes the accessibility of the transporter from cytoplasmic (inward) facing to extracellular (outward) facing. The inward and outward openings are mediated by two different sets of transmembrane helix interactions. Altogether, the conformational changes between these structures suggest that large ranges of motion may be required for substrate transport.
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Check<jmol>
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<jmolCheckbox>
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==About this Structure==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/b5/3b5z_consurf.spt"</scriptWhenChecked>
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3B5Z is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Salmonella_typhimurium Salmonella typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B5Z OCA].
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==Reference==
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</jmolCheckbox>
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Flexibility in the ABC transporter MsbA: Alternating access with a twist., Ward A, Reyes CL, Yu J, Roth CB, Chang G, Proc Natl Acad Sci U S A. 2007 Nov 27;104(48):19005-10. Epub 2007 Nov 16. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18024585 18024585]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3b5z ConSurf].
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[[Category: Salmonella typhimurium]]
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<div style="clear:both"></div>
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[[Category: Single protein]]
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__TOC__
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[[Category: Chang, G.]]
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</StructureSection>
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[[Category: Reyes, C L.]]
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[[Category: Large Structures]]
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[[Category: Roth, C B.]]
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[[Category: Salmonella enterica subsp. enterica serovar Typhimurium]]
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[[Category: Ward, A.]]
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[[Category: Chang G]]
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[[Category: Yu, J.]]
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[[Category: Reyes CL]]
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[[Category: ADP]]
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[[Category: Roth CB]]
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[[Category: abc transporter]]
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[[Category: Ward A]]
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[[Category: atp-binding]]
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[[Category: Yu J]]
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[[Category: hydrolase]]
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[[Category: inner membrane]]
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[[Category: lipid flippase]]
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[[Category: lipid transport]]
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[[Category: membrane]]
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[[Category: membrane protein]]
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[[Category: msba]]
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[[Category: nucleotide-binding]]
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[[Category: transmembrane]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 23 15:58:26 2008''
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Current revision

Crystal Structure of MsbA from Salmonella typhimurium with ADP Vanadate

PDB ID 3b5z

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