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3beo

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[[Image:3beo.jpg|left|200px]]
 
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{{Structure
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==A Structural Basis for the allosteric regulation of non-hydrolyzing UDP-GlcNAc 2-epimerases==
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|PDB= 3beo |SIZE=350|CAPTION= <scene name='initialview01'>3beo</scene>, resolution 1.700&Aring;
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<StructureSection load='3beo' size='340' side='right'caption='[[3beo]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
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|SITE= <scene name='pdbsite=AC1:Ud1+Binding+Site+For+Residue+A+372'>AC1</scene>, <scene name='pdbsite=AC2:Ud1+Binding+Site+For+Residue+B+372'>AC2</scene>, <scene name='pdbsite=AC3:Udp+Binding+Site+For+Residue+A+1081'>AC3</scene> and <scene name='pdbsite=AC4:Udp+Binding+Site+For+Residue+B+1081'>AC4</scene>
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=UD1:URIDINE-DIPHOSPHATE-N-ACETYLGLUCOSAMINE'>UD1</scene> and <scene name='pdbligand=UDP:URIDINE-5&#39;-DIPHOSPHATE'>UDP</scene>
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<table><tr><td colspan='2'>[[3beo]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_anthracis Bacillus anthracis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BEO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3BEO FirstGlance]. <br>
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|ACTIVITY= [http://en.wikipedia.org/wiki/UDP-N-acetylglucosamine_2-epimerase UDP-N-acetylglucosamine 2-epimerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.1.3.14 5.1.3.14]
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=UD1:URIDINE-DIPHOSPHATE-N-ACETYLGLUCOSAMINE'>UD1</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr>
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}}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3beo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3beo OCA], [https://pdbe.org/3beo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3beo RCSB], [https://www.ebi.ac.uk/pdbsum/3beo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3beo ProSAT]</span></td></tr>
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</table>
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'''A Structural Basis for the allosteric regulation of non-hydrolyzing UDP-GlcNAc 2-epimerases'''
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== Function ==
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[https://www.uniprot.org/uniprot/A0A348ACF3_BACAN A0A348ACF3_BACAN]
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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The non-hydrolysing bacterial UDP-N-acetylglucosamine 2-epimerase (UDP-GlcNAc 2-epimerase) catalyses the conversion of UDP-GlcNAc into UDP-N-acetylmannosamine, an intermediate in the biosynthesis of several cell-surface polysaccharides. This enzyme is allosterically regulated by its substrate UDP-GlcNAc. The structure of the ternary complex between the Bacillus anthracis UDP-GlcNAc 2-epimerase, its substrate UDP-GlcNAc and the reaction intermediate UDP, showed direct interactions between UDP and its substrate, and between the complex and highly conserved enzyme residues, identifying the allosteric site of the enzyme. The binding of UDP-GlcNAc is associated with conformational changes in the active site of the enzyme. Kinetic data and mutagenesis of the highly conserved UDP-GlcNAc-interacting residues confirm their importance in the substrate binding and catalysis of the enzyme. This constitutes the first example to our knowledge, of an enzymatic allosteric activation by direct interaction between the substrate and the allosteric activator.
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Check<jmol>
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<jmolCheckbox>
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==About this Structure==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/be/3beo_consurf.spt"</scriptWhenChecked>
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3BEO is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_anthracis Bacillus anthracis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BEO OCA].
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==Reference==
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</jmolCheckbox>
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A structural basis for the allosteric regulation of non-hydrolysing UDP-GlcNAc 2-epimerases., Velloso LM, Bhaskaran SS, Schuch R, Fischetti VA, Stebbins CE, EMBO Rep. 2008 Feb;9(2):199-205. Epub 2008 Jan 11. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18188181 18188181]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3beo ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Bacillus anthracis]]
[[Category: Bacillus anthracis]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: UDP-N-acetylglucosamine 2-epimerase]]
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[[Category: Bhaskaran SS]]
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[[Category: Bhaskaran, S S.]]
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[[Category: Fischetti VA]]
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[[Category: Fischetti, V A.]]
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[[Category: Schuch R]]
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[[Category: Schuch, R.]]
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[[Category: Stebbins CE]]
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[[Category: Stebbins, C E.]]
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[[Category: Velloso LM]]
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[[Category: Velloso, L M.]]
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[[Category: UD1]]
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[[Category: UDP]]
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[[Category: allosteric]]
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[[Category: epimerase]]
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[[Category: isomerase]]
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[[Category: regulation]]
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[[Category: udp-glcnac]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 23 15:59:18 2008''
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Current revision

A Structural Basis for the allosteric regulation of non-hydrolyzing UDP-GlcNAc 2-epimerases

PDB ID 3beo

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