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2i0n

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Structure of Dictyostelium discoideum Myosin VII SH3 domain with adjacent proline rich region

Structural highlights

2i0n is a 1 chain structure with sequence from Dictyostelium discoideum. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Solution NMR
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

MYOI_DICDI Myosins are actin-based motor molecules with ATPase activity. Involved in the early steps of phagocytosis and adhesion.^[1] ^[2] ^[3]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Myosins play essential roles in migration, cytokinesis, endocytosis, and adhesion. They are composed of a large N-terminal motor domain with ATPase and actin binding sites and C-terminal neck and tail regions, whose functional roles and structural context in the protein are less well characterized. The tail regions of myosins I, IV, VII, XII, and XV each contain a putative SH3 domain that may be involved in protein-protein interactions. SH3 domains are reported to bind proline-rich motifs, especially "PxxP" sequences, and such interactions serve regulatory functions. The activity of Src, PI3, and Itk kinases, for example, is regulated by intramolecular interactions between their SH3 domain and internal proline-rich sequences. Here, we use NMR spectroscopy to reveal the structure of a protein construct from Dictyostelium myosin VII (DdM7) spanning A1620-T1706, which contains its SH3 domain and adjacent proline-rich region. The SH3 domain forms the signature beta-barrel architecture found in other SH3 domains, with conserved tryptophan and tyrosine residues forming a hydrophobic pocket known to bind "PxxP" motifs. In addition, acidic residues in the RT or n-Src loops are available to interact with the basic anchoring residues that are typically found in ligands or proteins that bind SH3 domains. The DdM7 SH3 differs in the hydrophobicity of the second pocket formed by the 3(10) helix and following beta-strand, which contains polar rather than hydrophobic side chains. Most unusual, however, is that this domain binds its adjacent proline-rich region at a surface remote from the region previously identified to bind "PxxP" motifs. The interaction may affect the orientation of the tail without sacrificing the availability of the canonical "PxxP"-binding surface.

The SH3 domain of a M7 interacts with its C-terminal proline-rich region.,Wang Q, Deloia MA, Kang Y, Litchke C, Zhang N, Titus MA, Walters KJ Protein Sci. 2007 Feb;16(2):189-96. Epub 2006 Dec 22. PMID:17189480^[4]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Titus MA. A class VII unconventional myosin is required for phagocytosis. Curr Biol. 1999 Nov 18;9(22):1297-303. PMID:10574761
↑ Tuxworth RI, Weber I, Wessels D, Addicks GC, Soll DR, Gerisch G, Titus MA. A role for myosin VII in dynamic cell adhesion. Curr Biol. 2001 Mar 6;11(5):318-29. PMID:11267868
↑ Tuxworth RI, Stephens S, Ryan ZC, Titus MA. Identification of a myosin VII-talin complex. J Biol Chem. 2005 Jul 15;280(28):26557-64. Epub 2005 Apr 11. PMID:15826949 doi:http://dx.doi.org/M503699200
↑ Wang Q, Deloia MA, Kang Y, Litchke C, Zhang N, Titus MA, Walters KJ. The SH3 domain of a M7 interacts with its C-terminal proline-rich region. Protein Sci. 2007 Feb;16(2):189-96. Epub 2006 Dec 22. PMID:17189480 doi:10.1110/ps.062496807

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2i0n"

@@ Line 1: / Line 1: @@
 ==Structure of Dictyostelium discoideum Myosin VII SH3 domain with adjacent proline rich region==
-<StructureSection load='2i0n' size='340' side='right' caption='[[2i0n]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
+<StructureSection load='2i0n' size='340' side='right'caption='[[2i0n]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2i0n]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_11735 Atcc 11735]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I0N OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2I0N FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2i0n]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Dictyostelium_discoideum Dictyostelium discoideum]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I0N OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2I0N FirstGlance]. <br>
-</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">myoI ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=44689 ATCC 11735])</td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2i0n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i0n OCA], [http://pdbe.org/2i0n PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2i0n RCSB], [http://www.ebi.ac.uk/pdbsum/2i0n PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2i0n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i0n OCA], [https://pdbe.org/2i0n PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2i0n RCSB], [https://www.ebi.ac.uk/pdbsum/2i0n PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2i0n ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/MYOI_DICDI MYOI_DICDI]] Myosins are actin-based motor molecules with ATPase activity. Involved in the early steps of phagocytosis and adhesion.<ref>PMID:10574761</ref> <ref>PMID:11267868</ref> <ref>PMID:15826949</ref>
+[https://www.uniprot.org/uniprot/MYOI_DICDI MYOI_DICDI] Myosins are actin-based motor molecules with ATPase activity. Involved in the early steps of phagocytosis and adhesion.<ref>PMID:10574761</ref> <ref>PMID:11267868</ref> <ref>PMID:15826949</ref>
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
 Check<jmol>
   <jmolCheckbox>
-    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/i0/2i0n_consurf.spt"</scriptWhenChecked>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/i0/2i0n_consurf.spt"</scriptWhenChecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
   </jmolCheckbox>
-</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2i0n ConSurf].
 <div style="clear:both"></div>
 <div style="background-color:#fffaf0;">
@@ Line 29: / Line 30: @@
 ==See Also==
-*[[Myosin|Myosin]]
+*[[Myosin 3D Structures|Myosin 3D Structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Atcc 11735]]
+[[Category: Dictyostelium discoideum]]
-[[Category: Deloia, M A]]
+[[Category: Large Structures]]
-[[Category: Kang, Y]]
+[[Category: Deloia MA]]
-[[Category: Litchke, C]]
+[[Category: Kang Y]]
-[[Category: Titus, M A]]
+[[Category: Litchke C]]
-[[Category: Walters, K J]]
+[[Category: Titus MA]]
-[[Category: Wang, Q]]
+[[Category: Walters KJ]]
-[[Category: Beta-sheet loop]]
+[[Category: Wang Q]]
-[[Category: Structural protein]]

2i0n

From Proteopedia

Current revision

Structure of Dictyostelium discoideum Myosin VII SH3 domain with adjacent proline rich region

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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