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2b2a

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==Crystal Structure of the TEN domain of the Telomerase Reverse Transcriptase==
==Crystal Structure of the TEN domain of the Telomerase Reverse Transcriptase==
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<StructureSection load='2b2a' size='340' side='right' caption='[[2b2a]], [[Resolution|resolution]] 2.22&Aring;' scene=''>
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<StructureSection load='2b2a' size='340' side='right'caption='[[2b2a]], [[Resolution|resolution]] 2.22&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[2b2a]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Tetth Tetth]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B2A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2B2A FirstGlance]. <br>
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<table><tr><td colspan='2'>[[2b2a]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Tetrahymena_thermophila Tetrahymena thermophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B2A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2B2A FirstGlance]. <br>
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</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">TERT ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5911 TETTH])</td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.22&#8491;</td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/RNA-directed_DNA_polymerase RNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.49 2.7.7.49] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2b2a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2b2a OCA], [https://pdbe.org/2b2a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2b2a RCSB], [https://www.ebi.ac.uk/pdbsum/2b2a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2b2a ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2b2a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2b2a OCA], [http://pdbe.org/2b2a PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2b2a RCSB], [http://www.ebi.ac.uk/pdbsum/2b2a PDBsum]</span></td></tr>
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</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/TERT_TETTH TERT_TETTH]] Telomerase is a ribonucleoprotein enzyme essential for the replication of chromosome termini in most eukaryotes. It elongates telomeres. It is a reverse transcriptase that adds simple sequence repeats to chromosome ends by copying a template sequence within the RNA component of the enzyme (By similarity).
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[https://www.uniprot.org/uniprot/TERT_TETTS TERT_TETTS] Catalytic component of telomerase, an essential ribonucleoprotein enzyme that copies new telomeric repeats onto chromosome ends by repetitively synthesizing the short telomere-repeat sequence 5'-TTGGGG-3' using an RNA template component TER (PubMed:10944124, PubMed:15696174, PubMed:17322903, PubMed:20713447, PubMed:16462747). TERT is a reverse transcriptase that adds simple sequence repeats to chromosome ends by copying a template sequence within the RNA component of the enzyme (PubMed:10944124, PubMed:15696174, PubMed:17322903, PubMed:20713447, PubMed:16462747).<ref>PMID:10944124</ref> <ref>PMID:15696174</ref> <ref>PMID:16462747</ref> <ref>PMID:17322903</ref> <ref>PMID:20713447</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Telomerase, a ribonucleoprotein enzyme, adds telomeric DNA repeats to the ends of linear chromosomes. Here we report the first high-resolution structure of any portion of the telomerase reverse transcriptase, the telomerase essential N-terminal (TEN) domain from Tetrahymena thermophila. The structure, which seems to represent a novel protein fold, shows phylogenetically conserved amino acid residues in a groove on its surface. These residues are crucial for telomerase catalytic activity, and several of them are required for sequence-specific binding of a single-stranded telomeric DNA primer. The positively charged C terminus, which becomes ordered upon interaction with other macromolecules, is involved in binding RNA in a non-sequence-specific manner. The TEN domain's ability to bind both RNA and telomeric DNA, coupled with the notably strong effects on activity upon mutagenesis of single surface residues, suggest how this domain contributes to telomerase catalysis.
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Crystal structure of the essential N-terminal domain of telomerase reverse transcriptase.,Jacobs SA, Podell ER, Cech TR Nat Struct Mol Biol. 2006 Mar;13(3):218-25. Epub 2006 Feb 5. PMID:16462747<ref>PMID:16462747</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2b2a" style="background-color:#fffaf0;"></div>
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==See Also==
==See Also==
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*[[Reverse transcriptase|Reverse transcriptase]]
 
*[[Telomerase 3D structures|Telomerase 3D structures]]
*[[Telomerase 3D structures|Telomerase 3D structures]]
*[[Telomerase Reverse Transcriptase|Telomerase Reverse Transcriptase]]
*[[Telomerase Reverse Transcriptase|Telomerase Reverse Transcriptase]]
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: RNA-directed DNA polymerase]]
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[[Category: Large Structures]]
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[[Category: Tetth]]
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[[Category: Tetrahymena thermophila]]
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[[Category: Cech, T R]]
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[[Category: Cech TR]]
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[[Category: Jacobs, S A]]
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[[Category: Jacobs SA]]
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[[Category: Podell, E R]]
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[[Category: Podell ER]]
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[[Category: Reverse transcriptase]]
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[[Category: Rt]]
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[[Category: Telomerase]]
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[[Category: Tert]]
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[[Category: Transferase]]
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Current revision

Crystal Structure of the TEN domain of the Telomerase Reverse Transcriptase

PDB ID 2b2a

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