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1kq4

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CRYSTAL STRUCTURE OF A THY1-COMPLEMENTING PROTEIN (TM0449) FROM THERMOTOGA MARITIMA AT 2.25 A RESOLUTION

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Categories: Large Structures | Thermotoga maritima | Miller MD | Wilson IA

@@ Line 1: / Line 1: @@
-[[Image:1kq4.gif|left|200px]]
- {{Structure
+==CRYSTAL STRUCTURE OF A THY1-COMPLEMENTING PROTEIN (TM0449) FROM THERMOTOGA MARITIMA AT 2.25 A RESOLUTION==
-|PDB= 1kq4 |SIZE=350|CAPTION= <scene name='initialview01'>1kq4</scene>, resolution 2.25&Aring;
+<StructureSection load='1kq4' size='340' side='right'caption='[[1kq4]], [[Resolution|resolution]] 2.25&Aring;' scene=''>
-|SITE=
+== Structural highlights ==
-|LIGAND= <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>
+<table><tr><td colspan='2'>[[1kq4]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KQ4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1KQ4 FirstGlance]. <br>
-|ACTIVITY=
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.25&#8491;</td></tr>
-|GENE= TM0449 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2336 Thermotoga maritima])
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-|DOMAIN=<span class='plainlinks'>[http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=PRK00847 thyX]</span>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1kq4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kq4 OCA], [https://pdbe.org/1kq4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1kq4 RCSB], [https://www.ebi.ac.uk/pdbsum/1kq4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1kq4 ProSAT], [https://www.topsan.org/Proteins/JCSG/1kq4 TOPSAN]</span></td></tr>
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1kq4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kq4 OCA], [http://www.ebi.ac.uk/pdbsum/1kq4 PDBsum], [http://www.fli-leibniz.de/cgi-bin/ImgLib.pl?CODE=1kfv JenaLib], [http://www.rcsb.org/pdb/explore.do?structureId=1kq4 RCSB]</span>
+</table>
-}}
+== Function ==
+[https://www.uniprot.org/uniprot/THYX_THEMA THYX_THEMA] Catalyzes the formation of dTMP and tetrahydrofolate from dUMP and methylenetetrahydrofolate.
-'''Crystal Structure of Thy1-Complementing Protein (TM0449) from Thermotoga maritima at 2.25 A Resolution'''
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
-==Overview==
+  <jmolCheckbox>
-Structural genomics is emerging as a principal approach to define protein structure-function relationships. To apply this approach on a genomic scale, novel methods and technologies must be developed to determine large numbers of structures. We describe the design and implementation of a high-throughput structural genomics pipeline and its application to the proteome of the thermophilic bacterium Thermotoga maritima. By using this pipeline, we successfully cloned and attempted expression of 1,376 of the predicted 1,877 genes (73%) and have identified crystallization conditions for 432 proteins, comprising 23% of the T. maritima proteome. Representative structures from TM0423 glycerol dehydrogenase and TM0449 thymidylate synthase-complementing protein are presented as examples of final outputs from the pipeline.
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kq/1kq4_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
-==About this Structure==
+    <text>to colour the structure by Evolutionary Conservation</text>
-KQ4 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KQ4 OCA].
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1kq4 ConSurf].
-==Reference==
+<div style="clear:both"></div>
-Structural genomics of the Thermotoga maritima proteome implemented in a high-throughput structure determination pipeline., Lesley SA, Kuhn P, Godzik A, Deacon AM, Mathews I, Kreusch A, Spraggon G, Klock HE, McMullan D, Shin T, Vincent J, Robb A, Brinen LS, Miller MD, McPhillips TM, Miller MA, Scheibe D, Canaves JM, Guda C, Jaroszewski L, Selby TL, Elsliger MA, Wooley J, Taylor SS, Hodgson KO, Wilson IA, Schultz PG, Stevens RC, Proc Natl Acad Sci U S A. 2002 Sep 3;99(18):11664-9. Epub 2002 Aug 22. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12193646 12193646]
+__TOC__
-[[Category: Single protein]]
+</StructureSection>
+[[Category: Large Structures]]
 [[Category: Thermotoga maritima]]
-[[Category: JCSG, Joint Center for Structural Genomics.]]
+[[Category: Miller MD]]
-[[Category: Miller, M D.]]
+[[Category: Wilson IA]]
-[[Category: Wilson, I A.]]
-[[Category: flavin-adenine dinucleotide]]
-[[Category: jcsg]]
-[[Category: joint center for structural genomic]]
-[[Category: protein structure initiative]]
-[[Category: psi]]
-[[Category: structural genomic]]
-[[Category: thy1-complementing protein from thermotoga maritima]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Mar 25 23:24:28 2008''

1kq4

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CRYSTAL STRUCTURE OF A THY1-COMPLEMENTING PROTEIN (TM0449) FROM THERMOTOGA MARITIMA AT 2.25 A RESOLUTION

Structural highlights

Function

Evolutionary Conservation

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