|
|
(4 intermediate revisions not shown.) |
Line 1: |
Line 1: |
| + | |
| ==SOLUTION STRUCTURE OF CONOTOXIN Y-PIIIE FROM CONUS PURPURASCENS, NMR, 14 STRUCTURES== | | ==SOLUTION STRUCTURE OF CONOTOXIN Y-PIIIE FROM CONUS PURPURASCENS, NMR, 14 STRUCTURES== |
- | <StructureSection load='1as5' size='340' side='right' caption='[[1as5]], [[NMR_Ensembles_of_Models | 14 NMR models]]' scene=''> | + | <StructureSection load='1as5' size='340' side='right'caption='[[1as5]]' scene=''> |
| == Structural highlights == | | == Structural highlights == |
- | <table><tr><td colspan='2'>[[1as5]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Conpu Conpu]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AS5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1AS5 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1as5]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Conus_purpurascens Conus purpurascens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AS5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AS5 FirstGlance]. <br> |
- | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=HYP:4-HYDROXYPROLINE'>HYP</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
- | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1as5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1as5 OCA], [http://pdbe.org/1as5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1as5 RCSB], [http://www.ebi.ac.uk/pdbsum/1as5 PDBsum]</span></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HYP:4-HYDROXYPROLINE'>HYP</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1as5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1as5 OCA], [https://pdbe.org/1as5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1as5 RCSB], [https://www.ebi.ac.uk/pdbsum/1as5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1as5 ProSAT]</span></td></tr> |
| </table> | | </table> |
| == Function == | | == Function == |
- | [[http://www.uniprot.org/uniprot/CM3E_CONPU CM3E_CONPU]] Psi-conotoxins act on postsynaptic membranes, and act as non-competitive antagonist of nicotinic acetylcholine receptors (nAChR). Is more toxic than Psi-conotoxin PIIIF. Has paralytic activity when injected intraperitoneally into goldfish. | + | [https://www.uniprot.org/uniprot/CM3E_CONPU CM3E_CONPU] Psi-conotoxins act on postsynaptic membranes, and act as non-competitive antagonist of nicotinic acetylcholine receptors (nAChR). Is more toxic than Psi-conotoxin PIIIF. Has paralytic activity when injected intraperitoneally into goldfish. |
| <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
Line 21: |
Line 23: |
| __TOC__ | | __TOC__ |
| </StructureSection> | | </StructureSection> |
- | [[Category: Conpu]] | + | [[Category: Conus purpurascens]] |
- | [[Category: Foster, M P]] | + | [[Category: Large Structures]] |
- | [[Category: Ireland, C M]] | + | [[Category: Foster MP]] |
- | [[Category: Mitchell, S S]] | + | [[Category: Ireland CM]] |
- | [[Category: Olivera, B M]] | + | [[Category: Mitchell SS]] |
- | [[Category: Shon, K]] | + | [[Category: Olivera BM]] |
- | [[Category: Acetylcholine]] | + | [[Category: Shon K]] |
- | [[Category: Conotoxin]]
| + | |
- | [[Category: Neurotoxin]]
| + | |
| Structural highlights
Function
CM3E_CONPU Psi-conotoxins act on postsynaptic membranes, and act as non-competitive antagonist of nicotinic acetylcholine receptors (nAChR). Is more toxic than Psi-conotoxin PIIIF. Has paralytic activity when injected intraperitoneally into goldfish.
Publication Abstract from PubMed
The three-dimensional structure of conotoxin psi-PIIIE, a 24-amino acid peptide from Conus purpurascens, has been solved using two-dimensional (2D) 1H NMR spectroscopy. Conotoxin psi-PIIIE contains the same disulfide bonding pattern as the mu-conotoxins, which target skeletal muscle sodium channels, but has been shown to antagonize the acetylcholine gated cation channel through a noncompetitive mechanism. Structural information was obtained by the analysis of a series of 2D NOESY spectra as well as measurement of coupling constants from 1D 1H and PE-COSY NMR experiments. Molecular modeling calculations included the use of the distance geometry (DG) algorithm, simulated annealing techniques, and the restrained molecular dynamics method. The resulting structures are considerably similar to the previously published structures for the mu-conotoxins GIIIA and GIIIB, despite the lack of sequence conservation between conotoxin psi-PIIIE and the mu-conotoxins. The structure consists of a series of tight turns, each turn occurring in the position analogous to those of turns described in mu-GIIIA and mu-GIIIB. This suggests the disulfide bonding pattern is able to largely direct the structure of the peptides, creating a stable structural motif which allows extensive sequence substitution of non-cystine residues.
Three-dimensional solution structure of conotoxin psi-PIIIE, an acetylcholine gated ion channel antagonist.,Mitchell SS, Shon KJ, Foster MP, Davis DR, Olivera BM, Ireland CM Biochemistry. 1998 Feb 3;37(5):1215-20. PMID:9477946[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Mitchell SS, Shon KJ, Foster MP, Davis DR, Olivera BM, Ireland CM. Three-dimensional solution structure of conotoxin psi-PIIIE, an acetylcholine gated ion channel antagonist. Biochemistry. 1998 Feb 3;37(5):1215-20. PMID:9477946 doi:http://dx.doi.org/10.1021/bi972186t
|