This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


2l8a

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (06:54, 1 May 2024) (edit) (undo)
 
(3 intermediate revisions not shown.)
Line 1: Line 1:
 +
==Structure of a novel CBM3 lacking the calcium-binding site==
==Structure of a novel CBM3 lacking the calcium-binding site==
-
<StructureSection load='2l8a' size='340' side='right' caption='[[2l8a]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
+
<StructureSection load='2l8a' size='340' side='right'caption='[[2l8a]]' scene=''>
== Structural highlights ==
== Structural highlights ==
-
<table><tr><td colspan='2'>[[2l8a]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_globigii"_migula_1900 "bacillus globigii" migula 1900]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L8A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2L8A FirstGlance]. <br>
+
<table><tr><td colspan='2'>[[2l8a]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L8A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2L8A FirstGlance]. <br>
-
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3pzu|3pzu]], [[3pzv|3pzv]], [[3pzt|3pzt]]</td></tr>
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
-
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">eglS, bglC, gld, BSU18130 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 "Bacillus globigii" Migula 1900])</td></tr>
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2l8a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l8a OCA], [https://pdbe.org/2l8a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2l8a RCSB], [https://www.ebi.ac.uk/pdbsum/2l8a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2l8a ProSAT]</span></td></tr>
-
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cellulase Cellulase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4] </span></td></tr>
+
-
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2l8a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l8a OCA], [http://pdbe.org/2l8a PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2l8a RCSB], [http://www.ebi.ac.uk/pdbsum/2l8a PDBsum]</span></td></tr>
+
</table>
</table>
-
<div style="background-color:#fffaf0;">
+
== Function ==
-
== Publication Abstract from PubMed ==
+
[https://www.uniprot.org/uniprot/GUN2_BACSU GUN2_BACSU]
-
Cellulases participate in a number of biological events such as plant cell wall remodeling, nematode parasitism and microbial carbon uptake. Their ability to depolymerize crystalline cellulose is of great biotechnological interest for environmentally-compatible production of fuels from lignocellulosic biomass. However, industrial use of cellulases is somewhat limited both by their low catalytic efficiency and stability. In this work, we conducted a detailed functional and structural characterization of the thermostable cellulase 5A from Bacillus subtilis (BsCel5A), which consists of a GH5 catalytic domain fused to a family 3 carbohydrate-binding module (CBM3). NMR structural analysis revealed that the Bacillus CBM3 represents a new subfamily, which lacks the classical calcium-binding motif and variations in NMR frequencies in the presence of cellopentaose showed the importance of polar residues in the carbohydrate interaction. Together with the catalytic domain, the CBM3 forms a large planar surface for cellulose recognition, which conducts the substrate in a proper conformation to the active site and increases enzymatic efficiency. Notably, the manganese ion demonstrated to have a hyper-stabilizing effect on BsCel5A and by using deletion constructs and X-ray crystallography, we determined that this effect maps to a negatively charged motif located at the opposite face of the catalytic site.
+
-
 
+
-
Dissecting structure-function-stability relationships of a thermostable GH5-CBM3 cellulase from Bacillus subtilis 168.,Santos C, Paiva J, Sforca M, Neves J, Navarro R, Cota J, Akao P, Hoffmam ZB, Meza A, Smetana J, Nogueira M, Polikarpov I, Xavier-Neto J, Squina F, Ward RJ, Ruller R, Zeri A, Murakami MT Biochem J. 2011 Sep 1. PMID:21880019<ref>PMID:21880019</ref>
+
-
 
+
-
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+
-
</div>
+
-
<div class="pdbe-citations 2l8a" style="background-color:#fffaf0;"></div>
+
==See Also==
==See Also==
-
*[[Glucanase|Glucanase]]
+
*[[Glucanase 3D structures|Glucanase 3D structures]]
-
== References ==
+
-
<references/>
+
__TOC__
__TOC__
</StructureSection>
</StructureSection>
-
[[Category: Bacillus globigii migula 1900]]
+
[[Category: Bacillus subtilis]]
-
[[Category: Cellulase]]
+
[[Category: Large Structures]]
-
[[Category: Meza, A N]]
+
[[Category: Meza AN]]
-
[[Category: Murakami, M T]]
+
[[Category: Murakami MT]]
-
[[Category: Navarro, R Z]]
+
[[Category: Navarro RZ]]
-
[[Category: Neves, J L]]
+
[[Category: Neves JL]]
-
[[Category: Paiva, J H]]
+
[[Category: Paiva JH]]
-
[[Category: Santos, C R]]
+
[[Category: Santos CR]]
-
[[Category: Sforca, M L]]
+
[[Category: Sforca ML]]
-
[[Category: Zeri, A C]]
+
[[Category: Zeri AC]]
-
[[Category: Carbohydrate-binding module]]
+
-
[[Category: Family 3]]
+
-
[[Category: Hydrolase]]
+

Current revision

Structure of a novel CBM3 lacking the calcium-binding site

PDB ID 2l8a

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2l8a"
Personal tools