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| | ==THE STRUCTURE OF THE ISOLATED, CENTRAL HAIRPIN OF THE HDV ANTIGENOMIC RIBOZYME, NMR, 10 STRUCTURES== | | ==THE STRUCTURE OF THE ISOLATED, CENTRAL HAIRPIN OF THE HDV ANTIGENOMIC RIBOZYME, NMR, 10 STRUCTURES== |
| - | <StructureSection load='1ato' size='340' side='right' caption='[[1ato]], [[NMR_Ensembles_of_Models | 10 NMR models]]' scene=''> | + | <StructureSection load='1ato' size='340' side='right'caption='[[1ato]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1ato]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Hepatitis_delta_virus Hepatitis delta virus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ATO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ATO FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1ato]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Hepatitis_delta_virus Hepatitis delta virus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ATO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ATO FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ato FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ato OCA], [http://pdbe.org/1ato PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ato RCSB], [http://www.ebi.ac.uk/pdbsum/1ato PDBsum]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ato FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ato OCA], [https://pdbe.org/1ato PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ato RCSB], [https://www.ebi.ac.uk/pdbsum/1ato PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ato ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
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| | ==See Also== | | ==See Also== |
| - | *[[Ribozyme|Ribozyme]] | + | *[[Ribozyme 3D structures|Ribozyme 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Hepatitis delta virus]] | | [[Category: Hepatitis delta virus]] |
| - | [[Category: Heus, H A]] | + | [[Category: Large Structures]] |
| - | [[Category: Hilbers, C W]] | + | [[Category: Heus HA]] |
| - | [[Category: Kolk, M H]] | + | [[Category: Hilbers CW]] |
| - | [[Category: Central loop]] | + | [[Category: Kolk MH]] |
| - | [[Category: Ribozyme]]
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| - | [[Category: Rna]]
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| - | [[Category: Rna hairpin]]
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| Structural highlights
Publication Abstract from PubMed
The structure of an RNA hairpin containing a seven-nucleotide loop that is present in the self-cleaving sequence of hepatitis delta virus antigenomic RNA was determined by high resolution NMR spectroscopy. The loop, which is composed of only one purine and six pyrimidines, has a suprisingly stable structure, mainly supported by sugar hydroxyl hydrogen bonds and base-base and base-phosphate stacking interactions. Compared with the structurally well-determined, seven-membered anticodon loop in tRNA, the sharp turn which affects the required 180 degrees change in direction of the sugar-phosphate backbone in the loop is shifted one nucleotide in the 3' direction. This change in direction can be characterized as a reversed U-turn. It is expected that the reversed U-turn may be found frequently in other molecules as well. There is evidence for a new non-Watson-Crick UC base pair formed between the first and the last residue in the loop, while most of the other bases in the loop are pointing outwards making them accessible to solvent. From chemical modification, mutational and photocrosslinking studies, a similar picture develops for the structure of the hairpin in the active ribozyme indicating that the loop structure in the isolated hairpin and in the ribozyme is very similar.
The structure of the isolated, central hairpin of the HDV antigenomic ribozyme: novel structural features and similarity of the loop in the ribozyme and free in solution.,Kolk MH, Heus HA, Hilbers CW EMBO J. 1997 Jun 16;16(12):3685-92. PMID:9218809[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Kolk MH, Heus HA, Hilbers CW. The structure of the isolated, central hairpin of the HDV antigenomic ribozyme: novel structural features and similarity of the loop in the ribozyme and free in solution. EMBO J. 1997 Jun 16;16(12):3685-92. PMID:9218809 doi:10.1093/emboj/16.12.3685
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