1k0r

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[[Image:1k0r.gif|left|200px]]
 
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{{Structure
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==Crystal Structure of Mycobacterium tuberculosis NusA==
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|PDB= 1k0r |SIZE=350|CAPTION= <scene name='initialview01'>1k0r</scene>, resolution 1.70&Aring;
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<StructureSection load='1k0r' size='340' side='right'caption='[[1k0r]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
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<table><tr><td colspan='2'>[[1k0r]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K0R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1K0R FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
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|GENE= MTCY24A1, number Z95207.1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1773 Mycobacterium tuberculosis])
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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|DOMAIN=<span class='plainlinks'>[http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=cd02134 NusA_KH], [http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=pfam08529 NusA_N], [http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=PRK12327 nusA]</span>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1k0r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1k0r OCA], [https://pdbe.org/1k0r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1k0r RCSB], [https://www.ebi.ac.uk/pdbsum/1k0r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1k0r ProSAT]</span></td></tr>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1k0r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1k0r OCA], [http://www.ebi.ac.uk/pdbsum/1k0r PDBsum], [http://www.fli-leibniz.de/cgi-bin/ImgLib.pl?CODE=1kfv JenaLib], [http://www.rcsb.org/pdb/explore.do?structureId=1k0r RCSB]</span>
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</table>
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}}
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== Function ==
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[https://www.uniprot.org/uniprot/NUSA_MYCTU NUSA_MYCTU] Participates in both transcription termination and antitermination (By similarity).[HAMAP-Rule:MF_00945]
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'''Crystal Structure of Mycobacterium tuberculosis NusA'''
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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==Overview==
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<jmolCheckbox>
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Mycobacterium tuberculosis is the cause of tuberculosis in humans, a disease that affects over a one-third of the world's population. This slow-growing pathogen has only one ribosomal RNA operon, thus making its transcriptional apparatus a fundamentally interesting target for drug discovery. NusA binds to RNA polymerase and modulates several of the ribosomal RNA transcriptional processes. Here, we report the crystal structure of NusA, and reveal that the molecule consists of four domains. They are organised as two distinct entities. The N-terminal domain (residues 1 to 99) that resembles the B chain of the Rad50cd ATP binding cassette-ATPase (ABC-ATPase) and a C-terminal module (residues 108 to 329) consisting of a ribosomal S1 protein domain followed by two K homology domains. The S1 and KH domains are tightly integrated together to form an extensive RNA-binding structure, but are flexibly tethered to the N-terminal domain. The molecule's surfaces and architecture provide insights into RNA and polymerase interactions and the mechanism of pause site discrimination. They also allow us to rationalize certain termination-defective and cold shock-sensitive mutations in the nusA gene that have been studied in Escherichia coli.
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/k0/1k0r_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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==About this Structure==
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<text>to colour the structure by Evolutionary Conservation</text>
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1K0R is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K0R OCA].
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1k0r ConSurf].
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==Reference==
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<div style="clear:both"></div>
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Crystal structure of the transcription elongation/anti-termination factor NusA from Mycobacterium tuberculosis at 1.7 A resolution., Gopal B, Haire LF, Gamblin SJ, Dodson EJ, Lane AN, Papavinasasundaram KG, Colston MJ, Dodson G, J Mol Biol. 2001 Dec 14;314(5):1087-95. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11743725 11743725]
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Mycobacterium tuberculosis]]
[[Category: Mycobacterium tuberculosis]]
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[[Category: Single protein]]
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[[Category: Colston MJ]]
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[[Category: Colston, M J.]]
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[[Category: Dodson EJ]]
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[[Category: Dodson, E J.]]
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[[Category: Dodson G]]
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[[Category: Dodson, G.]]
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[[Category: Gamblin SJ]]
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[[Category: Gamblin, S J.]]
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[[Category: Gopal B]]
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[[Category: Gopal, B.]]
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[[Category: Haire LF]]
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[[Category: Haire, L F.]]
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[[Category: Lane AN]]
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[[Category: Lane, A N.]]
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[[Category: Papavinasasundaram KG]]
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[[Category: Papavinasasundaram, K G.]]
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[[Category: TBSGC, TB Structural Genomics Consortium.]]
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[[Category: protein structure initiative]]
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[[Category: psi]]
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[[Category: s1 domain]]
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[[Category: structural genomic]]
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[[Category: tb structural genomics consortium]]
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[[Category: tbsgc]]
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[[Category: two component arrangement]]
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[[Category: two k-homology domains.]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Mar 26 05:54:40 2008''
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Current revision

Crystal Structure of Mycobacterium tuberculosis NusA

PDB ID 1k0r

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