1ae1

<StructureSection load='1ae1' size='340' side='right' caption='[[1ae1]], [[Resolution|resolution]] 2.40&Aring;' scene=''>

<table><tr><td colspan='2'>[[1ae1]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Common_thornapple Common thornapple]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AE1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1AE1 FirstGlance]. <br>

</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>

<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Tropinone_reductase_II Tropinone reductase II], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.236 1.1.1.236] </span></td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ae1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ae1 OCA], [http://pdbe.org/1ae1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ae1 RCSB], [http://www.ebi.ac.uk/pdbsum/1ae1 PDBsum]</span></td></tr>

[[http://www.uniprot.org/uniprot/TRN1_DATST TRN1_DATST]] Catalyzes the stereospecific reduction of tropinone to tropine.

<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ae/1ae1_consurf.spt"</scriptWhenChecked>

</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].

<div style="background-color:#fffaf0;">

== Publication Abstract from PubMed ==

A pair of tropinone reductases (TRs) share 64% of the same amino acid residues and belong to the short-chain dehydrogenase/reductase family. In the synthesis of tropane alkaloids in several medicinal plants, the TRs reduce a carbonyl group of an alkaloid intermediate, tropinone, to hydroxy groups with different diastereomeric configurations. To clarify the structural basis for their different reaction stereospecificities, we determined the crystal structures of the two enzymes at 2.4- and 2.3-A resolutions. The overall folding of the two enzymes was almost identical. The conservation was not confined within the core domains that are conserved within the protein family but extended outside the core domain where each family member has its characteristic structure. The binding sites for the cofactor and the positions of the active site residues were well conserved between the two TRs. The substrate binding site was composed mostly of hydrophobic amino acids in both TRs, but the presence of different charged residues conferred different electrostatic environments on the two enzymes. A modeling study indicated that these charged residues play a major role in controlling the binding orientation of tropinone within the substrate binding site, thereby determining the stereospecificity of the reaction product. The results obtained herein raise the possibility that in certain cases different stereospecificities can be acquired in enzymes by changing a few amino acid residues within substrate binding sites.

Crystal structures of two tropinone reductases: different reaction stereospecificities in the same protein fold.,Nakajima K, Yamashita A, Akama H, Nakatsu T, Kato H, Hashimoto T, Oda J, Yamada Y Proc Natl Acad Sci U S A. 1998 Apr 28;95(9):4876-81. PMID:9560196<ref>PMID:9560196</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>

</div>

<div class="pdbe-citations 1ae1" style="background-color:#fffaf0;"></div>

==See Also==

*[[InhA|InhA]]

== References ==

<references/>

[[Category: Common thornapple]]

[[Category: Tropinone reductase II]]

[[Category: Akama, H]]

[[Category: Hashimoto, T]]

[[Category: Kato, H]]

[[Category: Nakajima, K]]

[[Category: Nakatsu, T]]

[[Category: Oda, J]]

[[Category: Yamada, Y]]

[[Category: Yamashita, A]]

[[Category: Tropane alkaloid biosynthesis]]