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| | ==NMR Structure of PcFK1== | | ==NMR Structure of PcFK1== |
| - | <StructureSection load='1x5v' size='340' side='right' caption='[[1x5v]], [[NMR_Ensembles_of_Models | 25 NMR models]]' scene=''> | + | <StructureSection load='1x5v' size='340' side='right'caption='[[1x5v]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1x5v]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Psaca Psaca]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1X5V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1X5V FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1x5v]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Psalmopoeus_cambridgei Psalmopoeus cambridgei]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1X5V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1X5V FirstGlance]. <br> |
| - | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=HSL:HOMOSERINE+LACTONE'>HSL</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1x5v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1x5v OCA], [http://pdbe.org/1x5v PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1x5v RCSB], [http://www.ebi.ac.uk/pdbsum/1x5v PDBsum]</span></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HSL:HOMOSERINE+LACTONE'>HSL</scene></td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1x5v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1x5v OCA], [https://pdbe.org/1x5v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1x5v RCSB], [https://www.ebi.ac.uk/pdbsum/1x5v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1x5v ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/TXFK1_PSACA TXFK1_PSACA]] Possess strong antiplasmodial activity against the intra-erythrocyte stage of P.falciparum in vitro. IC(50) for inhibiting P.falciparum growth is 1.59 uM. Interacts with infected and healthy erythrocytes. Does not lyse erythrocytes, is not cytotoxic to nucleated mammalian cells, and does not inhibit neuromuscular function. Has neither antibacterial nor antifungal activity.<ref>PMID:15304333</ref> | + | [https://www.uniprot.org/uniprot/NTA_PSACA NTA_PSACA] Possesses strong antiplasmodial activity against the intra-erythrocyte stage of P.falciparum in vitro. IC(50) for inhibiting P.falciparum growth is 1.59 uM. Interacts with infected and healthy erythrocytes. Does not lyse erythrocytes, is not cytotoxic to nucleated mammalian cells, and does not inhibit neuromuscular function. Has neither antibacterial nor antifungal activity.<ref>PMID:15304333</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Psaca]] | + | [[Category: Large Structures]] |
| - | [[Category: Camadro, J M]] | + | [[Category: Psalmopoeus cambridgei]] |
| - | [[Category: Chagot, B]] | + | [[Category: Camadro JM]] |
| - | [[Category: Choi, S J]] | + | [[Category: Chagot B]] |
| - | [[Category: Darbon, H]] | + | [[Category: Choi SJ]] |
| - | [[Category: Guette, C]] | + | [[Category: Darbon H]] |
| - | [[Category: Pimentel, C]] | + | [[Category: Guette C]] |
| - | [[Category: Inhibitory cystine knot]]
| + | [[Category: Pimentel C]] |
| - | [[Category: Toxin]]
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| Structural highlights
Function
NTA_PSACA Possesses strong antiplasmodial activity against the intra-erythrocyte stage of P.falciparum in vitro. IC(50) for inhibiting P.falciparum growth is 1.59 uM. Interacts with infected and healthy erythrocytes. Does not lyse erythrocytes, is not cytotoxic to nucleated mammalian cells, and does not inhibit neuromuscular function. Has neither antibacterial nor antifungal activity.[1]
Publication Abstract from PubMed
Psalmopeotoxin I (PcFK1) is a 33-amino-acid residue peptide isolated from the venom of the tarantula Psalmopoeus cambridgei. It has been recently shown to possess strong antiplasmodial activity against the intra-erythrocyte stage of Plasmodium falciparum in vitro. Although the molecular target for PcFK1 is not yet determined, this peptide does not lyse erythrocytes, is not cytotoxic to nucleated mammalian cells, and does not inhibit neuromuscular function. We investigated the structural properties of PcFK1 to help understand the unique mechanism of action of this peptide and to enhance its utility as a lead compound for rational development of new antimalarial drugs. In this paper, we have determined the three-dimensional solution structure by (1)H two-dimensional NMR means of recombinant PcFK1, which is shown to belong to the ICK structural superfamily with structural determinants common to several neurotoxins acting as ion channels effectors.
Solution structure of PcFK1, a spider peptide active against Plasmodium falciparum.,Pimentel C, Choi SJ, Chagot B, Guette C, Camadro JM, Darbon H Protein Sci. 2006 Mar;15(3):628-34. Epub 2006 Feb 1. PMID:16452619[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Choi SJ, Parent R, Guillaume C, Deregnaucourt C, Delarbre C, Ojcius DM, Montagne JJ, Celerier ML, Phelipot A, Amiche M, Molgo J, Camadro JM, Guette C. Isolation and characterization of Psalmopeotoxin I and II: two novel antimalarial peptides from the venom of the tarantula Psalmopoeus cambridgei. FEBS Lett. 2004 Aug 13;572(1-3):109-17. PMID:15304333 doi:http://dx.doi.org/10.1016/j.febslet.2004.07.019
- ↑ Pimentel C, Choi SJ, Chagot B, Guette C, Camadro JM, Darbon H. Solution structure of PcFK1, a spider peptide active against Plasmodium falciparum. Protein Sci. 2006 Mar;15(3):628-34. Epub 2006 Feb 1. PMID:16452619 doi:ps.051860606
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