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2mjf

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==Solution structure of the complex between the yeast Rsa1 and Hit1 proteins==
==Solution structure of the complex between the yeast Rsa1 and Hit1 proteins==
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<StructureSection load='2mjf' size='340' side='right' caption='[[2mjf]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
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<StructureSection load='2mjf' size='340' side='right'caption='[[2mjf]]' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[2mjf]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MJF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2MJF FirstGlance]. <br>
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<table><tr><td colspan='2'>[[2mjf]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MJF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MJF FirstGlance]. <br>
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</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2mjf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mjf OCA], [http://pdbe.org/2mjf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2mjf RCSB], [http://www.ebi.ac.uk/pdbsum/2mjf PDBsum]</span></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2mjf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mjf OCA], [https://pdbe.org/2mjf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2mjf RCSB], [https://www.ebi.ac.uk/pdbsum/2mjf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2mjf ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/RSA1_YEAST RSA1_YEAST]] Involved in a late nucleoplasmic step of 60S ribosomal subunit assembly.
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[https://www.uniprot.org/uniprot/RSA1_YEAST RSA1_YEAST] Involved in a late nucleoplasmic step of 60S ribosomal subunit assembly.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Biogenesis of eukaryotic box C/D small nucleolar ribonucleoprotein particles (C/D snoRNPs) involves conserved trans-acting factors, which are proposed to facilitate the assembly of the core proteins Snu13p/15.5K, Nop58p/NOP58, Nop56p/NOP56 and Nop1p/Fibrillarin on box C/D small nucleolar RNAs (C/D snoRNAs). In yeast, protein Rsa1 acts as a platform, interacting with both the RNA-binding core protein Snu13 and protein Pih1 of the Hsp82-R2TP chaperone complex. In this work, a proteomic approach coupled with functional and structural studies identifies protein Hit1 as a novel Rsa1p-interacting partner involved in C/D snoRNP assembly. Hit1p contributes to in vivo C/D snoRNA stability and pre-RNA maturation kinetics. It associates with U3 snoRNA precursors and influences its 3'-end processing. Remarkably, Hit1p is required to maintain steady-state levels of Rsa1p. This stabilizing activity is likely to be general across eukaryotic species, as the human protein ZNHIT3(TRIP3) showing sequence homology with Hit1p regulates the abundance of NUFIP1, the Rsa1p functional homolog. The nuclear magnetic resonance solution structure of the Rsa1p317-352-Hit1p70-164 complex reveals a novel mode of protein-protein association explaining the strong stability of the Rsa1p-Hit1p complex. Our biochemical data show that C/D snoRNAs and the core protein Nop58 can interact with the purified Snu13p-Rsa1p-Hit1p heterotrimer.
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Protein Hit1, a novel box C/D snoRNP assembly factor, controls cellular concentration of the scaffolding protein Rsa1 by direct interaction.,Rothe B, Saliou JM, Quinternet M, Back R, Tiotiu D, Jacquemin C, Loegler C, Schlotter F, Pena V, Eckert K, Morera S, Van Dorsselaer A, Branlant C, Massenet S, Sanglier-Cianferani S, Manival X, Charpentier B Nucleic Acids Res. 2014 Aug 28. pii: gku612. PMID:25170085<ref>PMID:25170085</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2mjf" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Back, R]]
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[[Category: Large Structures]]
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[[Category: Jacquemin, C]]
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[[Category: Saccharomyces cerevisiae S288C]]
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[[Category: Manival, X]]
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[[Category: Back R]]
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[[Category: Quinternet, M]]
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[[Category: Jacquemin C]]
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[[Category: Roth, B]]
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[[Category: Manival X]]
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[[Category: Protein binding]]
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[[Category: Quinternet M]]
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[[Category: Roth B]]

Current revision

Solution structure of the complex between the yeast Rsa1 and Hit1 proteins

PDB ID 2mjf

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