1ri5

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[[Image:1ri5.gif|left|200px]]
 
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{{Structure
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==Structure and mechanism of mRNA cap (guanine N-7) methyltransferase==
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|PDB= 1ri5 |SIZE=350|CAPTION= <scene name='initialview01'>1ri5</scene>, resolution 2.10&Aring;
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<StructureSection load='1ri5' size='340' side='right'caption='[[1ri5]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND=
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<table><tr><td colspan='2'>[[1ri5]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Encephalitozoon_cuniculi Encephalitozoon cuniculi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RI5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RI5 FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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|GENE= ECU10_0380 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6035 Encephalitozoon cuniculi])
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ri5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ri5 OCA], [https://pdbe.org/1ri5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ri5 RCSB], [https://www.ebi.ac.uk/pdbsum/1ri5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ri5 ProSAT], [https://www.topsan.org/Proteins/NYSGXRC/1ri5 TOPSAN]</span></td></tr>
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|DOMAIN=<span class='plainlinks'>[http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=pfam03291 Pox_MCEL]</span>
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ri5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ri5 OCA], [http://www.ebi.ac.uk/pdbsum/1ri5 PDBsum], [http://www.fli-leibniz.de/cgi-bin/ImgLib.pl?CODE=1kfv JenaLib], [http://www.rcsb.org/pdb/explore.do?structureId=1ri5 RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/MCES_ENCCU MCES_ENCCU] mRNA-capping methyltransferase that methylates the N7 position of the added guanosine to the 5'-cap structure of mRNAs. Binds RNA containing 5'-terminal GpppC.<ref>PMID:15760890</ref>
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== Evolutionary Conservation ==
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'''Structure and mechanism of mRNA cap (guanine N-7) methyltransferase'''
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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==Overview==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ri/1ri5_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ri5 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
A suite of crystal structures is reported for a cellular mRNA cap (guanine-N7) methyltransferase in complex with AdoMet, AdoHcy, and the cap guanylate. Superposition of ligand complexes suggests an in-line mechanism of methyl transfer, albeit without direct contacts between the enzyme and either the N7 atom of guanine (the attacking nucleophile), the methyl carbon of AdoMet, or the sulfur of AdoMet/AdoHcy (the leaving group). The structures indicate that catalysis of cap N7 methylation is accomplished by optimizing proximity and orientation of the substrates, assisted by a favorable electrostatic environment. The enzyme-ligand structures, together with new mutational data, fully account for the biochemical specificity of the cap guanine-N7 methylation reaction, an essential and defining step of eukaryotic mRNA synthesis.
A suite of crystal structures is reported for a cellular mRNA cap (guanine-N7) methyltransferase in complex with AdoMet, AdoHcy, and the cap guanylate. Superposition of ligand complexes suggests an in-line mechanism of methyl transfer, albeit without direct contacts between the enzyme and either the N7 atom of guanine (the attacking nucleophile), the methyl carbon of AdoMet, or the sulfur of AdoMet/AdoHcy (the leaving group). The structures indicate that catalysis of cap N7 methylation is accomplished by optimizing proximity and orientation of the substrates, assisted by a favorable electrostatic environment. The enzyme-ligand structures, together with new mutational data, fully account for the biochemical specificity of the cap guanine-N7 methylation reaction, an essential and defining step of eukaryotic mRNA synthesis.
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==About this Structure==
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Structure and mechanism of mRNA cap (guanine-N7) methyltransferase.,Fabrega C, Hausmann S, Shen V, Shuman S, Lima CD Mol Cell. 2004 Jan 16;13(1):77-89. PMID:14731396<ref>PMID:14731396</ref>
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1RI5 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Encephalitozoon_cuniculi Encephalitozoon cuniculi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RI5 OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Structure and mechanism of mRNA cap (guanine-N7) methyltransferase., Fabrega C, Hausmann S, Shen V, Shuman S, Lima CD, Mol Cell. 2004 Jan 16;13(1):77-89. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14731396 14731396]
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</div>
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<div class="pdbe-citations 1ri5" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Encephalitozoon cuniculi]]
[[Category: Encephalitozoon cuniculi]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Burley, S K.]]
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[[Category: Burley SK]]
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[[Category: Fabrega, C.]]
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[[Category: Fabrega C]]
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[[Category: Hausmann, S.]]
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[[Category: Hausmann S]]
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[[Category: Lima, C D.]]
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[[Category: Lima CD]]
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[[Category: NYSGXRC, New York Structural GenomiX Research Consortium.]]
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[[Category: Shen V]]
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[[Category: Shen, V.]]
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[[Category: Shuman S]]
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[[Category: Shuman, S.]]
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[[Category: cap]]
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[[Category: m7g]]
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[[Category: messenger rna cap]]
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[[Category: methyltransferase]]
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[[Category: new york structural genomix research consortium]]
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[[Category: nysgxrc]]
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[[Category: protein structure initiative]]
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[[Category: psi]]
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[[Category: rna]]
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[[Category: structural genomic]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Mar 26 06:02:55 2008''
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Current revision

Structure and mechanism of mRNA cap (guanine N-7) methyltransferase

PDB ID 1ri5

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