1rtt

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[[Image:1rtt.gif|left|200px]]
 
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{{Structure
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==Crystal structure determination of a putative NADH-dependent reductase using sulfur anomalous signal==
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|PDB= 1rtt |SIZE=350|CAPTION= <scene name='initialview01'>1rtt</scene>, resolution 1.28&Aring;
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<StructureSection load='1rtt' size='340' side='right'caption='[[1rtt]], [[Resolution|resolution]] 1.28&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
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<table><tr><td colspan='2'>[[1rtt]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RTT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RTT FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.28&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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|DOMAIN=<span class='plainlinks'>[http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=COG0431 COG0431]</span>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1rtt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rtt OCA], [https://pdbe.org/1rtt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1rtt RCSB], [https://www.ebi.ac.uk/pdbsum/1rtt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1rtt ProSAT], [https://www.topsan.org/Proteins/NYSGXRC/1rtt TOPSAN]</span></td></tr>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1rtt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rtt OCA], [http://www.ebi.ac.uk/pdbsum/1rtt PDBsum], [http://www.fli-leibniz.de/cgi-bin/ImgLib.pl?CODE=1kfv JenaLib], [http://www.rcsb.org/pdb/explore.do?structureId=1rtt RCSB]</span>
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</table>
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}}
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== Function ==
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[https://www.uniprot.org/uniprot/FMNRE_PSEAE FMNRE_PSEAE] Has NAD(P)H-dependent FMN reductase activity.<ref>PMID:16552139</ref>
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'''Crystal structure determination of a putative NADH-dependent reductase using sulfur anomalous signal'''
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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==Overview==
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<jmolCheckbox>
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The availability of high-intensity synchrotron facilities, technological advances in data-collection techniques and improved data-reduction and crystallographic software have ushered in a new era in high-throughput macromolecular crystallography. Here, the de novo automated crystal structure determination at 1.28 A resolution of an NAD(P)H-dependent FMN reductase flavoprotein from Pseudomonas aeruginosa PA01-derived protein Q9I4D4 using the anomalous signal from an unusually small number of S atoms is reported. Although this protein lacks the flavodoxin key fingerprint motif [(T/S)XTGXT], it has been confirmed to bind flavin mononucleotide and the binding site was identified via X-ray crystallography. This protein contains a novel flavin mononucleotide-binding site GSLRSGSYN, which has not been previously reported. Detailed statistics pertaining to sulfur phasing and other factors contributing to structure determination are discussed. Structural comparisons of the apoenzyme and the protein complexed with flavin mononucleotide show conformational changes on cofactor binding. NADPH-dependent activity has been confirmed with biochemical assays.
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/rt/1rtt_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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==About this Structure==
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<text>to colour the structure by Evolutionary Conservation</text>
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1RTT is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_pao1 Pseudomonas aeruginosa pao1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RTT OCA].
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1rtt ConSurf].
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==Reference==
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<div style="clear:both"></div>
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Structure determination of an FMN reductase from Pseudomonas aeruginosa PA01 using sulfur anomalous signal., Agarwal R, Bonanno JB, Burley SK, Swaminathan S, Acta Crystallogr D Biol Crystallogr. 2006 Apr;62(Pt 4):383-91. Epub 2006, Mar 18. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16552139 16552139]
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== References ==
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[[Category: Pseudomonas aeruginosa pao1]]
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<references/>
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[[Category: Single protein]]
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__TOC__
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[[Category: Agarwal, R.]]
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</StructureSection>
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[[Category: Burley, S K.]]
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[[Category: Large Structures]]
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[[Category: NYSGXRC, New York Structural GenomiX Research Consortium.]]
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[[Category: Pseudomonas aeruginosa PAO1]]
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[[Category: Swaminathan, S.]]
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[[Category: Agarwal R]]
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[[Category: new york structural genomix research consortium]]
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[[Category: Burley SK]]
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[[Category: nysgxrc]]
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[[Category: Swaminathan S]]
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[[Category: protein structure initiative]]
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[[Category: psi]]
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[[Category: putative reductase]]
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[[Category: sad with sulfur]]
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[[Category: structural genomic]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Mar 26 06:03:22 2008''
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Current revision

Crystal structure determination of a putative NADH-dependent reductase using sulfur anomalous signal

PDB ID 1rtt

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