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1s9s
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| - | [[Image:1s9s.gif|left|200px]] | ||
| - | + | ==SOLUTION STRUCTURE OF MLV PSI SITE== | |
| - | + | <StructureSection load='1s9s' size='340' side='right'caption='[[1s9s]]' scene=''> | |
| - | + | == Structural highlights == | |
| - | + | <table><tr><td colspan='2'>[[1s9s]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Moloney_murine_leukemia_virus Moloney murine leukemia virus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S9S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1S9S FirstGlance]. <br> | |
| - | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> | |
| - | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1s9s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1s9s OCA], [https://pdbe.org/1s9s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1s9s RCSB], [https://www.ebi.ac.uk/pdbsum/1s9s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1s9s ProSAT]</span></td></tr> | |
| - | + | </table> | |
| - | + | <div style="background-color:#fffaf0;"> | |
| - | + | == Publication Abstract from PubMed == | |
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The full length, positive-strand genome of the Moloney Murine Leukemia Virus contains a "core encapsidation signal" that is essential for efficient genome packaging during virus assembly. We have determined the structure of a 101-nucleotide RNA that contains this signal (called mPsi) using a novel isotope-edited NMR approach. The method is robust and should be generally applicable to larger RNAs. mPsi folds into three stem loops, two of which (SL-C and SL-D) co-stack to form an extended helix. The third stem loop (SL-B) is connected to SL-C by a flexible, four-nucleotide linker. The structure contains five mismatched base-pairs, an unusual C.CG base-triple platform, and a novel "A-minor K-turn," in which unpaired adenosine bases A340 and A341 of a GGAA bulge pack in the minor groove of a proximal stem, and a bulged distal uridine (U319) forms a hydrogen bond with the phosphodiester of A341. Phylogenetic analyses indicate that these essential structural elements are conserved among the murine C-type retroviruses. | The full length, positive-strand genome of the Moloney Murine Leukemia Virus contains a "core encapsidation signal" that is essential for efficient genome packaging during virus assembly. We have determined the structure of a 101-nucleotide RNA that contains this signal (called mPsi) using a novel isotope-edited NMR approach. The method is robust and should be generally applicable to larger RNAs. mPsi folds into three stem loops, two of which (SL-C and SL-D) co-stack to form an extended helix. The third stem loop (SL-B) is connected to SL-C by a flexible, four-nucleotide linker. The structure contains five mismatched base-pairs, an unusual C.CG base-triple platform, and a novel "A-minor K-turn," in which unpaired adenosine bases A340 and A341 of a GGAA bulge pack in the minor groove of a proximal stem, and a bulged distal uridine (U319) forms a hydrogen bond with the phosphodiester of A341. Phylogenetic analyses indicate that these essential structural elements are conserved among the murine C-type retroviruses. | ||
| - | + | NMR structure of the 101-nucleotide core encapsidation signal of the Moloney murine leukemia virus.,D'Souza V, Dey A, Habib D, Summers MF J Mol Biol. 2004 Mar 19;337(2):427-42. PMID:15003457<ref>PMID:15003457</ref> | |
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| - | NMR structure of the 101-nucleotide core encapsidation signal of the Moloney murine leukemia virus., D'Souza V, Dey A, Habib D, Summers MF | + | |
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| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| + | </div> | ||
| + | <div class="pdbe-citations 1s9s" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Moloney murine leukemia virus]] | ||
| + | [[Category: D'Souza V]] | ||
| + | [[Category: Dey A]] | ||
| + | [[Category: Habib D]] | ||
| + | [[Category: Summers MF]] | ||
Current revision
SOLUTION STRUCTURE OF MLV PSI SITE
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