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| | ==NMR STRUCTURE OF INTRAMOLECULAR DIMER ANTIFREEZE PROTEIN RD3, 40 SA STRUCTURES== | | ==NMR STRUCTURE OF INTRAMOLECULAR DIMER ANTIFREEZE PROTEIN RD3, 40 SA STRUCTURES== |
| - | <StructureSection load='1c89' size='340' side='right' caption='[[1c89]], [[NMR_Ensembles_of_Models | 40 NMR models]]' scene=''> | + | <StructureSection load='1c89' size='340' side='right'caption='[[1c89]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1c89]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Antarctic_eel_pout Antarctic eel pout]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1C89 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1C89 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1c89]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pachycara_brachycephalum Pachycara brachycephalum]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1C89 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1C89 FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1c8a|1c8a]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1c89 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1c89 OCA], [http://pdbe.org/1c89 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1c89 RCSB], [http://www.ebi.ac.uk/pdbsum/1c89 PDBsum]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1c89 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1c89 OCA], [https://pdbe.org/1c89 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1c89 RCSB], [https://www.ebi.ac.uk/pdbsum/1c89 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1c89 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/ANP3_RHIDE ANP3_RHIDE]] Contributes to protect fish blood from freezing at subzero sea water temperatures. Lowers the blood freezing point. Binds to nascent ice crystals and prevents further growth (By similarity). | + | [https://www.uniprot.org/uniprot/ANP3_LYCDA ANP3_LYCDA] Contributes to protect fish blood from freezing at subzero sea water temperatures. Lowers the blood freezing point. Binds to nascent ice crystals and prevents further growth (By similarity). |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | ==See Also== | | ==See Also== |
| - | *[[Antifreeze protein|Antifreeze protein]] | + | *[[Antifreeze protein 3D structures|Antifreeze protein 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Antarctic eel pout]] | + | [[Category: Large Structures]] |
| - | [[Category: Miura, K]] | + | [[Category: Pachycara brachycephalum]] |
| - | [[Category: Tsuda, S]] | + | [[Category: Miura K]] |
| - | [[Category: Antifreeze]] | + | [[Category: Tsuda S]] |
| - | [[Category: Antifreeze protein]]
| + | |
| - | [[Category: Ice binding protein]]
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| - | [[Category: Thermal hysteresis protein]]
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| Structural highlights
Function
ANP3_LYCDA Contributes to protect fish blood from freezing at subzero sea water temperatures. Lowers the blood freezing point. Binds to nascent ice crystals and prevents further growth (By similarity).
Publication Abstract from PubMed
The structure of a new antifreeze protein (AFP) variant, RD3, from antarctic eel pout (Rhigophila dearborni) with enhanced activity has been determined for the first time by nuclear magnetic resonance spectroscopy. RD3 comprises a unique translational topology of two homologous type III AFP globular domains, each containing one flat, ice binding plane. The ice binding plane of the N domain is located approximately 3.5 A "behind" that of the C domain. The two ice binding planes are located laterally with an angle of 32 +/- 12 degrees between the planes. These results suggest that the C domain plane of RD3 binds first to the ice [1010] prism plane in the <0001> direction, which induces successive ice binding of the N domain in the <0101> direction. This manner of ice binding caused by the unique structural topology of RD3 is thought to be crucial for the significant enhancement of antifreeze activity, especially at low AFP concentrations.
NMR analysis of type III antifreeze protein intramolecular dimer. Structural basis for enhanced activity.,Miura K, Ohgiya S, Hoshino T, Nemoto N, Suetake T, Miura A, Spyracopoulos L, Kondo H, Tsuda S J Biol Chem. 2001 Jan 12;276(2):1304-10. PMID:11010977[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Miura K, Ohgiya S, Hoshino T, Nemoto N, Suetake T, Miura A, Spyracopoulos L, Kondo H, Tsuda S. NMR analysis of type III antifreeze protein intramolecular dimer. Structural basis for enhanced activity. J Biol Chem. 2001 Jan 12;276(2):1304-10. PMID:11010977 doi:10.1074/jbc.M007902200
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