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| | ==THE NMR SOLUTION STRUCTURE OF THE PHEROMONE ER-2 FROM THE CILIATED PROTOZOAN EUPLOTES RAIKOVI== | | ==THE NMR SOLUTION STRUCTURE OF THE PHEROMONE ER-2 FROM THE CILIATED PROTOZOAN EUPLOTES RAIKOVI== |
| - | <StructureSection load='1erd' size='340' side='right' caption='[[1erd]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='1erd' size='340' side='right'caption='[[1erd]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1erd]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Eupra Eupra]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ERD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ERD FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1erd]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Euplotes_raikovi Euplotes raikovi]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ERD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ERD FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1erd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1erd OCA], [http://pdbe.org/1erd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1erd RCSB], [http://www.ebi.ac.uk/pdbsum/1erd PDBsum]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 20 models</td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1erd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1erd OCA], [https://pdbe.org/1erd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1erd RCSB], [https://www.ebi.ac.uk/pdbsum/1erd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1erd ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/MER2_EUPRA MER2_EUPRA]] Mating ciliate pheromones (or gamones) are diffusible extracellular communication signals that distinguish different intraspecific classes of cells commonly referred to as 'mating types'. They prepare the latter for conjugation by changing their cell surface properties. | + | [https://www.uniprot.org/uniprot/MER2_EUPRA MER2_EUPRA] Mating ciliate pheromones (or gamones) are diffusible extracellular communication signals that distinguish different intraspecific classes of cells commonly referred to as 'mating types'. They prepare the latter for conjugation by changing their cell surface properties. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Eupra]] | + | [[Category: Euplotes raikovi]] |
| - | [[Category: Bradshaw, R A]] | + | [[Category: Large Structures]] |
| - | [[Category: Luginbuhl, P]] | + | [[Category: Bradshaw RA]] |
| - | [[Category: Luporini, P]] | + | [[Category: Luginbuhl P]] |
| - | [[Category: Ortenzi, C]] | + | [[Category: Luporini P]] |
| - | [[Category: Ottiger, M]] | + | [[Category: Ortenzi C]] |
| - | [[Category: Szyperski, T]] | + | [[Category: Ottiger M]] |
| - | [[Category: Wuthrich, K]] | + | [[Category: Szyperski T]] |
| - | [[Category: Pheromone]]
| + | [[Category: Wuthrich K]] |
| Structural highlights
Function
MER2_EUPRA Mating ciliate pheromones (or gamones) are diffusible extracellular communication signals that distinguish different intraspecific classes of cells commonly referred to as 'mating types'. They prepare the latter for conjugation by changing their cell surface properties.
Publication Abstract from PubMed
The NMR structure of the pheromone Er-2 from the ciliated protozoan Euplotes raikovi has been determined in aqueous solution. The structure of this 40-residue protein was calculated with the distance geometry program DIANA from 621 distance constraints and 89 dihedral angle constraints; the program OPAL was employed for the energy minimization. For a group of 20 conformers used to characterize the solution structure, the average pairwise RMS deviation from the mean structure calculated for the backbone heavy atoms N, C alpha, and C' of residues 3-37 was 0.31 A. The molecular architecture is dominated by an up-down-up bundle of 3 short helices of residues 5-11, 14-20, and 23-33, which is similar to the structures of the homologous pheromones Er-1 and Er-10. Novel structural features include a well-defined N-cap on the first helix, a 1-residue deletion in the second helix resulting in the formation of a 3(10)-helix rather than an alpha-helix as found in Er-1 and Er-10, and the simultaneous presence of 2 different conformations for the C-terminal tetrapeptide segment, i.e., a major conformation with the Leu 39-Pro 40 peptide bond in the trans form and a minor conformation with this peptide bond in the cis form.
The NMR solution structure of the pheromone Er-2 from the ciliated protozoan Euplotes raikovi.,Ottiger M, Szyperski T, Luginbuhl P, Ortenzi C, Luporini P, Bradshaw RA, Wuthrich K Protein Sci. 1994 Sep;3(9):1515-26. PMID:7833811[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Ottiger M, Szyperski T, Luginbuhl P, Ortenzi C, Luporini P, Bradshaw RA, Wuthrich K. The NMR solution structure of the pheromone Er-2 from the ciliated protozoan Euplotes raikovi. Protein Sci. 1994 Sep;3(9):1515-26. PMID:7833811
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