1k3e

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (07:44, 7 February 2024) (edit) (undo)
 
(4 intermediate revisions not shown.)
Line 1: Line 1:
 +
==Type III secretion chaperone CesT==
==Type III secretion chaperone CesT==
-
<StructureSection load='1k3e' size='340' side='right' caption='[[1k3e]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
+
<StructureSection load='1k3e' size='340' side='right'caption='[[1k3e]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
-
<table><tr><td colspan='2'>[[1k3e]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K3E OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1K3E FirstGlance]. <br>
+
<table><tr><td colspan='2'>[[1k3e]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_O157:H7 Escherichia coli O157:H7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K3E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1K3E FirstGlance]. <br>
-
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1k3e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1k3e OCA], [http://pdbe.org/1k3e PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1k3e RCSB], [http://www.ebi.ac.uk/pdbsum/1k3e PDBsum]</span></td></tr>
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1k3e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1k3e OCA], [https://pdbe.org/1k3e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1k3e RCSB], [https://www.ebi.ac.uk/pdbsum/1k3e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1k3e ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
-
[[http://www.uniprot.org/uniprot/CEST_ECOLX CEST_ECOLX]] Chaperone for the type III secretion of Tir. Probably stabilizes the protein, prevents inappropriate protein-protein interactions and aids in secretion (By similarity).
+
[https://www.uniprot.org/uniprot/CEST_ECO57 CEST_ECO57] Chaperone for the type III secretion of Tir. Probably stabilizes the protein, prevents inappropriate protein-proteininteractions and aids in secretion (By similarity).
-
<div style="background-color:#fffaf0;">
+
-
== Publication Abstract from PubMed ==
+
-
Several Gram-negative bacterial pathogens have evolved a type III secretion system to deliver virulence effector proteins directly into eukaryotic cells, a process essential for disease. This specialized secretion process requires customized chaperones specific for particular effector proteins. The crystal structures of the enterohemorrhagic Escherichia coli O157:H7 Tir-specific chaperone CesT and the Salmonella enterica SigD-specific chaperone SigE reveal a common overall fold and formation of homodimers. Site-directed mutagenesis suggests that variable, delocalized hydrophobic surfaces observed on the chaperone homodimers are responsible for specific binding to a particular effector protein. Isothermal titration calorimetry studies of Tir-CesT and enzymatic activity profiles of SigD-SigE indicate that the effector proteins are not globally unfolded in the presence of their cognate chaperones.
+
-
 
+
-
Structural and biochemical characterization of the type III secretion chaperones CesT and SigE.,Luo Y, Bertero MG, Frey EA, Pfuetzner RA, Wenk MR, Creagh L, Marcus SL, Lim D, Sicheri F, Kay C, Haynes C, Finlay BB, Strynadka NC Nat Struct Biol. 2001 Dec;8(12):1031-6. PMID:11685226<ref>PMID:11685226</ref>
+
-
 
+
-
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+
-
</div>
+
-
<div class="pdbe-citations 1k3e" style="background-color:#fffaf0;"></div>
+
-
== References ==
+
-
<references/>
+
__TOC__
__TOC__
</StructureSection>
</StructureSection>
-
[[Category: Bacillus coli migula 1895]]
+
[[Category: Escherichia coli O157:H7]]
-
[[Category: Bertero, M]]
+
[[Category: Large Structures]]
-
[[Category: Creagh, L]]
+
[[Category: Bertero M]]
-
[[Category: Finlay, B B]]
+
[[Category: Creagh L]]
-
[[Category: Frey, E A]]
+
[[Category: Finlay BB]]
-
[[Category: Lim, D]]
+
[[Category: Frey EA]]
-
[[Category: Luo, Y]]
+
[[Category: Lim D]]
-
[[Category: Marcus, S L]]
+
[[Category: Luo Y]]
-
[[Category: Pfuetzner, R A]]
+
[[Category: Marcus SL]]
-
[[Category: Strynadka, N C.J]]
+
[[Category: Pfuetzner RA]]
-
[[Category: Wenk, M R]]
+
[[Category: Strynadka NCJ]]
-
[[Category: Chaperone]]
+
[[Category: Wenk MR]]
-
[[Category: Intimin receptor]]
+
-
[[Category: Secretion]]
+
-
[[Category: Type iii]]
+

Current revision

Type III secretion chaperone CesT

PDB ID 1k3e

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1k3e"
Personal tools