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| - | ==P-ELEMENT SOMATIC INHIBITOR PROTEIN COMPLEX WITH U1-70K PROLINE-RICH PEPTIDE== | + | |
| - | <StructureSection load='2bn5' size='340' side='right' caption='[[2bn5]], [[NMR_Ensembles_of_Models | 28 NMR models]]' scene=''> | + | ==P-Element Somatic Inhibitor Protein Complex with U1-70k proline-rich peptide== |
| | + | <StructureSection load='2bn5' size='340' side='right'caption='[[2bn5]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2bn5]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Drome Drome]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BN5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2BN5 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2bn5]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BN5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BN5 FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2bn6|2bn6]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2bn5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bn5 OCA], [http://pdbe.org/2bn5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2bn5 RCSB], [http://www.ebi.ac.uk/pdbsum/2bn5 PDBsum]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2bn5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bn5 OCA], [https://pdbe.org/2bn5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2bn5 RCSB], [https://www.ebi.ac.uk/pdbsum/2bn5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2bn5 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/RU17_DROME RU17_DROME]] Mediates the splicing of pre-mRNA by binding to the stem loop I region of U1-snRNA.<ref>PMID:15611175</ref> | + | [https://www.uniprot.org/uniprot/Q7JPS0_DROSP Q7JPS0_DROSP] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | | | |
| | ==See Also== | | ==See Also== |
| - | *[[Nucleoprotein|Nucleoprotein]] | + | *[[Nucleoprotein 3D structures|Nucleoprotein 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Drome]] | + | [[Category: Drosophila melanogaster]] |
| - | [[Category: Butler, P J.G]] | + | [[Category: Large Structures]] |
| - | [[Category: Ignjatovic, T]] | + | [[Category: Butler PJG]] |
| - | [[Category: Nagai, K]] | + | [[Category: Ignjatovic T]] |
| - | [[Category: Neuhaus, D]] | + | [[Category: Nagai K]] |
| - | [[Category: Yang, J C]] | + | [[Category: Neuhaus D]] |
| - | [[Category: Complex]]
| + | [[Category: Yang J-C]] |
| - | [[Category: Inhibitor]]
| + | |
| - | [[Category: Nuclear protein]]
| + | |
| - | [[Category: Nuclear protein-complex]]
| + | |
| - | [[Category: Proline-rich peptide]]
| + | |
| - | [[Category: Protein-protein interaction]]
| + | |
| - | [[Category: Splicing]]
| + | |
| Structural highlights
Function
Q7JPS0_DROSP
Publication Abstract from PubMed
P-element transposition in Drosophila is regulated by tissue-specific alternative splicing of the P-element transposase pre-mRNA. In somatic cells, the P-element somatic inhibitor (PSI) protein binds to exon 3 of the pre-mRNA and recruits U1 small nuclear ribonucleoprotein (snRNP) to the F1 pseudo-splice site. This abrogates binding of U1 snRNP to the genuine 5' splice site, thereby preventing excision of the third intron. Two homologous short sequences, referred to as the A and B boxes, near the C terminus of PSI bind to U1-70k protein within U1 snRNP. We have now mapped the AB box-binding site of U1-70k to a short proline-rich sequence at the C terminus. Our NMR study shows that the B box forms an anti-parallel helical hairpin in which four highly conserved aromatic residues form a cluster on one face of the first helix. This hydrophobic cluster interacts extensively with the proline-rich region of the U1-70k protein.
Structural basis of the interaction between P-element somatic inhibitor and U1-70k essential for the alternative splicing of P-element transposase.,Ignjatovic T, Yang JC, Butler J, Neuhaus D, Nagai K J Mol Biol. 2005 Aug 5;351(1):52-65. PMID:15990112[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Ignjatovic T, Yang JC, Butler J, Neuhaus D, Nagai K. Structural basis of the interaction between P-element somatic inhibitor and U1-70k essential for the alternative splicing of P-element transposase. J Mol Biol. 2005 Aug 5;351(1):52-65. PMID:15990112 doi:S0022-2836(05)00543-7
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