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| - | [[Image:2hek.gif|left|200px]] | |
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| - | {{Structure
| + | ==Crystal structure of O67745, a hypothetical protein from Aquifex aeolicus at 2.0 A resolution.== |
| - | |PDB= 2hek |SIZE=350|CAPTION= <scene name='initialview01'>2hek</scene>, resolution 1.997Å
| + | <StructureSection load='2hek' size='340' side='right'caption='[[2hek]], [[Resolution|resolution]] 2.00Å' scene=''> |
| - | |SITE= <scene name='pdbsite=AC1:Po4+Binding+Site+For+Residue+A+501'>AC1</scene>, <scene name='pdbsite=AC2:Po4+Binding+Site+For+Residue+B+502'>AC2</scene>, <scene name='pdbsite=AC3:Po4+Binding+Site+For+Residue+B+503'>AC3</scene>, <scene name='pdbsite=AC4:Po4+Binding+Site+For+Residue+B+504'>AC4</scene>, <scene name='pdbsite=AC5:Zn+Binding+Site+For+Residue+B+601'>AC5</scene>, <scene name='pdbsite=AC6:Zn+Binding+Site+For+Residue+A+602'>AC6</scene>, <scene name='pdbsite=AC7:Br+Binding+Site+For+Residue+B+603'>AC7</scene>, <scene name='pdbsite=AC8:Cl+Binding+Site+For+Residue+A+604'>AC8</scene>, <scene name='pdbsite=AC9:Cl+Binding+Site+For+Residue+B+605'>AC9</scene>, <scene name='pdbsite=BC1:Cl+Binding+Site+For+Residue+A+606'>BC1</scene>, <scene name='pdbsite=BC2:Cl+Binding+Site+For+Residue+B+607'>BC2</scene>, <scene name='pdbsite=BC3:Cl+Binding+Site+For+Residue+B+608'>BC3</scene>, <scene name='pdbsite=BC4:Gdp+Binding+Site+For+Residue+A+401'>BC4</scene>, <scene name='pdbsite=BC5:Gdp+Binding+Site+For+Residue+A+402'>BC5</scene>, <scene name='pdbsite=BC6:Gol+Binding+Site+For+Residue+A+403'>BC6</scene>, <scene name='pdbsite=BC7:Gol+Binding+Site+For+Residue+A+404'>BC7</scene>, <scene name='pdbsite=BC8:Gol+Binding+Site+For+Residue+B+405'>BC8</scene>, <scene name='pdbsite=BC9:Gol+Binding+Site+For+Residue+A+406'>BC9</scene>, <scene name='pdbsite=CC1:Gol+Binding+Site+For+Residue+A+407'>CC1</scene>, <scene name='pdbsite=CC2:Gol+Binding+Site+For+Residue+A+408'>CC2</scene>, <scene name='pdbsite=CC3:Gol+Binding+Site+For+Residue+B+409'>CC3</scene> and <scene name='pdbsite=CC4:Gol+Binding+Site+For+Residue+A+410'>CC4</scene>
| + | == Structural highlights == |
| - | |LIGAND= <scene name='pdbligand=BR:BROMIDE+ION'>BR</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GDP:GUANOSINE-5'-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
| + | <table><tr><td colspan='2'>[[2hek]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Aquifex_aeolicus Aquifex aeolicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HEK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2HEK FirstGlance]. <br> |
| - | |ACTIVITY=
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.997Å</td></tr> |
| - | |GENE=
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BR:BROMIDE+ION'>BR</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| - | |DOMAIN=<span class='plainlinks'>[http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=cd00077 HDc], [http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=COG1078 COG1078]</span>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2hek FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hek OCA], [https://pdbe.org/2hek PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2hek RCSB], [https://www.ebi.ac.uk/pdbsum/2hek PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2hek ProSAT], [https://www.topsan.org/Proteins/BSGC/2hek TOPSAN]</span></td></tr> |
| - | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2hek FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hek OCA], [http://www.ebi.ac.uk/pdbsum/2hek PDBsum], [http://www.fli-leibniz.de/cgi-bin/ImgLib.pl?CODE=1kfv JenaLib], [http://www.rcsb.org/pdb/explore.do?structureId=2hek RCSB]</span>
| + | </table> |
| - | }}
| + | == Function == |
| - | | + | [https://www.uniprot.org/uniprot/O67745_AQUAE O67745_AQUAE] |
| - | '''Crystal structure of O67745, a hypothetical protein from Aquifex aeolicus at 2.0 A resolution.'''
| + | == Evolutionary Conservation == |
| - | | + | [[Image:Consurf_key_small.gif|200px|right]] |
| - | | + | Check<jmol> |
| - | ==Overview== | + | <jmolCheckbox> |
| - | Using single-wavelength anomalous dispersion data obtained from a gold-derivatized crystal, the X-ray crystal structure of the protein 067745_AQUAE from the prokaryotic organism Aquifex aeolicus has been determined to a resolution of 2.0 A. Amino-acid residues 1-371 of the 44 kDa protein were identified by Pfam as an HD domain and a member of the metal-dependent phosphohydrolase superfamily (accession No. PF01966). Although three families from this large and diverse group of enzymatic proteins are represented in the PDB, the structure of 067745_AQUAE reveals a unique fold that is unlike the others and that is likely to represent a new subfamily, further organizing the families and characterizing the proteins. Data are presented that provide the first insights into the structural organization of the proteins within this clan and a distal alternative GDP-binding domain outside the metal-binding active site is proposed.
| + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/he/2hek_consurf.spt"</scriptWhenChecked> |
| - | | + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> |
| - | ==About this Structure== | + | <text>to colour the structure by Evolutionary Conservation</text> |
| - | 2HEK is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Aquifex_aeolicus Aquifex aeolicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HEK OCA].
| + | </jmolCheckbox> |
| - | | + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2hek ConSurf]. |
| - | ==Reference== | + | <div style="clear:both"></div> |
| - | Structure of O67745_AQUAE, a hypothetical protein from Aquifex aeolicus., Oganesyan V, Adams PD, Jancarik J, Kim R, Kim SH, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 May 1;63(Pt, 5):369-74. Epub 2007 Apr 28. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17565173 17565173]
| + | __TOC__ |
| | + | </StructureSection> |
| | [[Category: Aquifex aeolicus]] | | [[Category: Aquifex aeolicus]] |
| - | [[Category: Single protein]] | + | [[Category: Large Structures]] |
| - | [[Category: Adams, P D.]] | + | [[Category: Adams PD]] |
| - | [[Category: BSGC, Berkeley Structural Genomics Center.]]
| + | [[Category: Jancarik J]] |
| - | [[Category: Jancarik, J.]] | + | [[Category: Kim R]] |
| - | [[Category: Kim, R.]] | + | [[Category: Kim SH]] |
| - | [[Category: Kim, S H.]] | + | [[Category: Oganesyan V]] |
| - | [[Category: Oganesyan, V.]] | + | |
| - | [[Category: berkeley structural genomics center]]
| + | |
| - | [[Category: bsgc]]
| + | |
| - | [[Category: predominantly alpha helical protein with gdp binding site and active site being far from each other]]
| + | |
| - | [[Category: protein structure initiative]]
| + | |
| - | [[Category: psi]]
| + | |
| - | [[Category: structural genomic]]
| + | |
| - | [[Category: unknown function]]
| + | |
| - | | + | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Mar 26 06:28:42 2008''
| + | |
