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4o0q

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==Apo structure of a methyltransferase component involved in O-demethylation==
==Apo structure of a methyltransferase component involved in O-demethylation==
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<StructureSection load='4o0q' size='340' side='right' caption='[[4o0q]], [[Resolution|resolution]] 1.92&Aring;' scene=''>
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<StructureSection load='4o0q' size='340' side='right'caption='[[4o0q]], [[Resolution|resolution]] 1.92&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4o0q]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4O0Q OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4O0Q FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4o0q]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Desulfitobacterium_hafniense_DCB-2 Desulfitobacterium hafniense DCB-2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4O0Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4O0Q FirstGlance]. <br>
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</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4o1f|4o1f]], [[4o1e|4o1e]]</td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.92&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4o0q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4o0q OCA], [http://pdbe.org/4o0q PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4o0q RCSB], [http://www.ebi.ac.uk/pdbsum/4o0q PDBsum]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4o0q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4o0q OCA], [https://pdbe.org/4o0q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4o0q RCSB], [https://www.ebi.ac.uk/pdbsum/4o0q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4o0q ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/B8FW00_DESHD B8FW00_DESHD]
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O-Demethylation by acetogenic or organohalide-respiring bacteria leads to the formation of methyltetrahydrofolate from aromatic methyl ethers. O-Demethylases, which are cobalamin-dependent, three-component enzyme systems, catalyse methyl-group transfers from aromatic methyl ethers to tetrahydrofolate via methylcobalamin intermediates. In this study, crystal structures of the tetrahydrofolate-binding methyltransferase module from a Desulfitobacterium hafniense DCB-2 O-demethylase were determined both in complex with tetrahydrofolate and the product methyltetrahydrofolate. While these structures are similar to previously determined methyltransferase structures, the position of key active-site residues is subtly altered. A strictly conserved Asn is displaced to establish a putative proton-transfer network between the substrate N5 and solvent. It is proposed that this supports the efficient catalysis of methyltetrahydrofolate formation, which is necessary for efficient O-demethylation.
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Structures of the methyltransferase component of Desulfitobacterium hafniense DCB-2 O-demethylase shed light on methyltetrahydrofolate formation.,Sjuts H, Dunstan MS, Fisher K, Leys D Acta Crystallogr D Biol Crystallogr. 2015 Sep 1;71(Pt 9):1900-8. doi:, 10.1107/S1399004715013061. Epub 2015 Aug 25. PMID:26327380<ref>PMID:26327380</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 4o0q" style="background-color:#fffaf0;"></div>
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==See Also==
==See Also==
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*[[Dihydropteroate synthase|Dihydropteroate synthase]]
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*[[Dihydropteroate synthase 3D structures|Dihydropteroate synthase 3D structures]]
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Dunstan, M S]]
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[[Category: Desulfitobacterium hafniense DCB-2]]
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[[Category: Fisher, K]]
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[[Category: Large Structures]]
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[[Category: Leys, D]]
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[[Category: Dunstan MS]]
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[[Category: Sjuts, H]]
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[[Category: Fisher K]]
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[[Category: Methyltransferase]]
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[[Category: Leys D]]
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[[Category: Thf/mthf]]
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[[Category: Sjuts H]]
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[[Category: Tim barrel]]
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[[Category: Transferase]]
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Apo structure of a methyltransferase component involved in O-demethylation

PDB ID 4o0q

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