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| | ==X-ray structure of AAV2 OBD-AAVS1 complex 2:1== | | ==X-ray structure of AAV2 OBD-AAVS1 complex 2:1== |
| - | <StructureSection load='5byg' size='340' side='right' caption='[[5byg]], [[Resolution|resolution]] 2.50Å' scene=''> | + | <StructureSection load='5byg' size='340' side='right'caption='[[5byg]], [[Resolution|resolution]] 2.50Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5byg]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5BYG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5BYG FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5byg]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Adeno-associated_virus_2_Srivastava/1982 Adeno-associated virus 2 Srivastava/1982] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5BYG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5BYG FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5byg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5byg OCA], [http://pdbe.org/5byg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5byg RCSB], [http://www.ebi.ac.uk/pdbsum/5byg PDBsum]</span></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5byg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5byg OCA], [https://pdbe.org/5byg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5byg RCSB], [https://www.ebi.ac.uk/pdbsum/5byg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5byg ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/REP78_AAV2S REP78_AAV2S]] Plays an essential role in the initiation of viral DNA synthesis. Binds specifically to an inverted terminal repeat element (ITR) on the 3' and 5' ends of the viral DNA, where it cleaves a site specifically to generate a priming site for initiation of the synthesis of a complementary strand. Plays also a role as transcriptional regulator, DNA helicase and as key factors in site-specific integration of the viral genome. Regulates host PKA activity by interacting with host PRKX as a mechanism of interfering with helper virus propagation and promoting its own replication. Inhibits the host cell cycle G1/S, S and G2/M transitions. These arrests may provide essential cellular factors for viral DNA replication.<ref>PMID:10944118</ref> | + | [https://www.uniprot.org/uniprot/REP78_AAV2S REP78_AAV2S] Plays an essential role in the initiation of viral DNA synthesis. Binds specifically to an inverted terminal repeat element (ITR) on the 3' and 5' ends of the viral DNA, where it cleaves a site specifically to generate a priming site for initiation of the synthesis of a complementary strand. Plays also a role as transcriptional regulator, DNA helicase and as key factors in site-specific integration of the viral genome. Regulates host PKA activity by interacting with host PRKX as a mechanism of interfering with helper virus propagation and promoting its own replication. Inhibits the host cell cycle G1/S, S and G2/M transitions. These arrests may provide essential cellular factors for viral DNA replication.<ref>PMID:10944118</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Escalante, C R]] | + | [[Category: Adeno-associated virus 2 Srivastava/1982]] |
| - | [[Category: Musayev, F N]] | + | [[Category: Homo sapiens]] |
| - | [[Category: Dna binding protein-dna complex]] | + | [[Category: Large Structures]] |
| - | [[Category: Protein-dna complex]] | + | [[Category: Escalante CR]] |
| | + | [[Category: Musayev FN]] |
| Structural highlights
Function
REP78_AAV2S Plays an essential role in the initiation of viral DNA synthesis. Binds specifically to an inverted terminal repeat element (ITR) on the 3' and 5' ends of the viral DNA, where it cleaves a site specifically to generate a priming site for initiation of the synthesis of a complementary strand. Plays also a role as transcriptional regulator, DNA helicase and as key factors in site-specific integration of the viral genome. Regulates host PKA activity by interacting with host PRKX as a mechanism of interfering with helper virus propagation and promoting its own replication. Inhibits the host cell cycle G1/S, S and G2/M transitions. These arrests may provide essential cellular factors for viral DNA replication.[1]
Publication Abstract from PubMed
Adeno-Associated virus (AAV) is the only eukaryotic virus with the property of establishing latency by integrating site-specifically into the human genome. The integration site known as AAVS1 is located in chromosome 19 and contains multiple GCTC repeats that are recognized by the AAV non-structural Rep proteins. These proteins are multifunctional, with an N-terminal origin-binding domain (OBD) and a helicase domain joined together by a short linker. As a first step to understand the process of site-specific integration, we set up to characterize the recognition and assembly of Rep68 onto the AAVS1 site. We first determined the X-ray structure of AAV-2 Rep68 OBD in complex with the AAVS1 DNA site. Specificity is achieved through the interaction of a glycine-rich loop that binds the major groove and an alpha-helix that interacts with a downstream minor groove on the same face of the DNA. Although the structure shows a complex with three OBD molecules bound to the AAVS1 site, we show using analytical centrifugation and electron microscopy that the full length Rep68 forms a heptameric complex. Moreover, we determine that a minimum of two direct repeats is required to form a stable complex and melt DNA. Finally, we show that although the individual domains bind DNA poorly, complex assembly requires oligomerization and cooperative between its OBD, helicase and the linker domains.
Structural Insights into the Assembly of the Adeno-Associated Virus Type 2 Rep68 Protein on the Integration site AAVS1.,Musayev FN, Zarate-Perez F, Bishop C, Burgner JW 2nd, Escalante CR J Biol Chem. 2015 Sep 14. pii: jbc.M115.669960. PMID:26370092[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Saudan P, Vlach J, Beard P. Inhibition of S-phase progression by adeno-associated virus Rep78 protein is mediated by hypophosphorylated pRb. EMBO J. 2000 Aug 15;19(16):4351-61. PMID:10944118 doi:http://dx.doi.org/10.1093/emboj/19.16.4351
- ↑ Musayev FN, Zarate-Perez F, Bishop C, Burgner JW 2nd, Escalante CR. Structural Insights into the Assembly of the Adeno-Associated Virus Type 2 Rep68 Protein on the Integration site AAVS1. J Biol Chem. 2015 Sep 14. pii: jbc.M115.669960. PMID:26370092 doi:http://dx.doi.org/10.1074/jbc.M115.669960
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